[1]罗清,於艳萍,卢业飞,等.基于SSR标记的杜鹃品种亲缘关系分析[J].北方园艺,2017,41(19):111-117.[doi:10.11937/bfyy.20170663]
　LUO Qing,YU Yanping,LU Yefei,et al.Analysis of Genetic Relationship of Wild Rhododendron Species Based on SSR Marker[J].Northern Horticulture,2017,41(19):111-117.[doi:10.11937/bfyy.20170663]
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基于SSR标记的杜鹃品种亲缘关系分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年19 页码: 111-117 栏目: 出版日期: 2017-10-15
Title:
Analysis of Genetic Relationship of Wild Rhododendron Species Based on SSR Marker
作者:
罗清; 於艳萍; 卢业飞; 梁春
广西壮族自治区亚热带作物研究所，广西 南宁 530001
Author(s):
LUO Qing; YU Yanping; LU Yefei; LIANG Chun
Guangxi Subtropical Crops Research Institute，Nanning，Guangxi 530001
关键词:
杜鹃; 亲缘关系分析; SSR
Keywords:
Rhododendron; genetic relationship analysis; SSR
DOI:
10.11937/bfyy.20170663
文献标志码:
A
摘要:
杜鹃野生种花色多样、种类丰富，在杜鹃杂交育种中具有良好的应用前景，有必要对杜鹃种质资源的亲缘关系进行研究。现以19份野生杜鹃品种为试材，采用SSR分子标记技术，研究了19份野生杜鹃品种间的亲缘关系，以期为合理挖掘和利用杜鹃种质资源提供理论基础，为杂交育种亲本选择提供科学依据。结果表明：10对SSR引物共扩增出138条谱带，其中多态性谱带134条，多态性比率为97.06%。遗传相似系数(GS)在0.502 7~0.918 0，平均值为0.678 9，表明19份杜鹃品种的亲缘关系较远、具有较广泛的遗传基础。当相似系数为0.70时，19份参试材料划分为五大类群，与经典分类学一致。
Abstract:
Wild Rhododendron species possess rich varieties and colors，and have a good application prospect in Rhododendron hybridization.Therefore，it is necessary to study the genetic relationship of Rhododendron germplasm.The genetic relationships among the 19 wild Rhododendron varieties were analyzed using SSR，in order to provide a theoretical basis for rational excavation and utilization of Rhododendron germplasm resources，and to provide a scientific basis for parental selection of hybrid breeding.The results showed that，totally 138 bands were amplified in the PCR reactions，including 134 polymorphic bands with the polymorphic rate of 97.06%.Among the analysis，the genetic similarity coefficients ranged from 0.502 7 to 0.918 0 with average of 0.678 9，showing that the 19 Rhododendron species have far genetic relationship，indicating abundance genetic diversity among these species.With the similarity coefficients of 0.70，19 Rhododendron species could be divided into five groups，which were consistent with classical taxonomy.

参考文献/References:

[1]周兰英,王永清,张丽,等.46种杜鹃花属植物表型性状的数量分类[J].林业科学,2009,45(8):67-75.

[2]耿玉英.中国杜鹃花解读[M].北京:中国林业出版社,2008:1-10.
[3]MOORE S S,SARGEANT L L,KING T J,et al.The conservation of dinucleotide microsatellites among mammalian genomes allows the use of heterologous PCR primer pairs in closely related species[J].Genomics,1991,10(3):654-660.
[4]贾会霞,姬慧娟,胡建军,等.杨树新品种的SSR指纹图谱构建和倍性检测[J].林业科学,2015,51(2):70-79.
[5]吴敏,杜红岩,乌云塔娜,等.杜仲基因组微卫星特征及SSR标记开发[J].林业科学研究,2015,28(3):387-393.
[6]黄海燕,杜红岩,乌云塔娜,等.基于杜仲转录组序列的SSR分子标记的开发[J].林业科学,2013,49(5):176-181.
[7]KSLIA R,RAI M,KALIA S,et al.Microsatellite markers:an overview of the recent progress in plants[J].Euphytica,2011,177(3):309-334.
[8]MRAZEK J,GUO X,SHAH A.Simple sequence repeats in prokaryotic genomes[J].PNAS,2007,104(20):8472-8477.
[9]CHOUDHARY S,THAKUR S,SAINI R Q,et al.Development and characterization of genomic microsatellite markers in Rhododendron arboreum[J].Conservation Genetics Resources,2014,6:937-940.
[10]张振,张含国,莫迟,等.红松转录组SSR分析及EST-SSR标记开发[J].林业科学,2015,51(8):114-120.
[11]程小毛,黄晓霞.SSR标记开发及其在植物中的应用[J].中国农学通报,2011,27(5):304-307.
[12]蔡长福,刘改秀,成仿云,等.牡丹遗传作图最适F1分离群体的选择[J].北京林业大学学报,2015,37(3):139-147.
[13]黄永艺,唐敏,张志荣,等.云南莲瓣兰主栽品种SSR指纹图谱的构建和遗传差异分析[J].热带亚热带植物学报,2015,23(3):236-244.
[14]任羽,王呈丹,徐世松,等.石斛兰亲缘关系的SSR分析[J].热带作物学报,2013,34(7):1252-1256.
[15]ALLEN G，FLORES V M，KRASYNANSKI S，et al.A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide[J].Nature Protocols,2006,1(5):2320-2325.
[16]DENDAUW J.Development of sequenced tagged microsatellite site (STMS) markers in azalea[J].Acta Horticulturae,2001,546:193-197.
[17]XUE Q W,YUAN H,CHUN L L,et al.Cross-amplification and characterization of microsatellite loci for the genus rhododendron[J].Hort Science,2010,45(9):1394-1397.
[18]IQBAL M J,PADEN D W，RAYBUM A L,et al.Assessment of genetic relationships among Rhododendron species,varieties and hybrids by RAPD analysis[J].Scientia Horticulturae,1995,63(3-4):215-223.
[19]郑宇,何天友,陈凌艳,等.比利时杜鹃栽培品种(系)的ISSR分析[J].福建农林大学学报(自然科学版),2011,40(3):271-275.
[20]李红英,李康琴,胥猛,等.北美鹅掌楸EST-SSR跨属间通用性[J].东北林业大学学报,2011,39(2):28-30.
[21]DENDAUW J,de R J,de L M,et al.Identification of 33 Chinese Rhododendron species using matk sequences and AFLP data[J].Acta Horticulturae,2002,572:169-177.
[22]赵凯,王德元,张文娟,等.都支杜鹃遗传多样性的ISSR分析[J].植物分类与资源学报,2013,35(5):578-584.
[23]周泓.杜鹃花品种资源多样性研究及品种分类体系构建[D].杭州:浙江大学,2012.
[24]刘旭颖,沈向群,张艳红,等.9种耐寒杜鹃花亲缘关系ISSR分析[J].西北农业学报,2010,19(7):89-92.

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备注/Memo

第一作者简介：罗清（1986-），女，硕士，助理研究员，研究方向为植物种质资源收集及育种与栽培。E-mail：lqlq522@163.com.

收稿日期：2017-04-06

更新日期/Last Update: 2017-10-12