[1]赵思思,刘兴菊,李雪雁,等.基于成熟期的梨品种遗传多样性SSR分析[J].北方园艺,2017,41(16):43-49.[doi:10.11937/bfyy.20170631]
　ZHAO Sisi,LIU Xingju,LI Xueyan,et al.Genetic Diversity of SSR Analysis of Pear Cultivars Based on Maturity[J].Northern Horticulture,2017,41(16):43-49.[doi:10.11937/bfyy.20170631]
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基于成熟期的梨品种遗传多样性SSR分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年16 页码: 43-49 栏目: 出版日期: 2017-08-30
Title:
Genetic Diversity of SSR Analysis of Pear Cultivars Based on Maturity
作者:
赵思思; 刘兴菊; 李雪雁; 王晓叶; 梁海永; 刘铭
河北农业大学 林学院，河北省林木种质资源与森林保护重点实验室，河北 保定 071000
Author(s):
ZHAO Sisi; LIU Xingju; LI Xueyan; WANG Xiaoye; LIANG Haiyong; LIU Ming
College of Forestry，Agricultural University of Hebei/Key Lab of Genetic Resources of Forest and Forest Protection of Hebei Province，Baoding，Hebei 071000
关键词:
梨; 简单重复序列（SSR）; 毛细管电泳; 成熟期; 遗传多样性
Keywords:
Pyrus L.; simple sequences repeat(SSR); capillary electrophoresis; maturity; genetic diversity
DOI:
10.11937/bfyy.20170631
文献标志码:
A
摘要:
以63个梨主栽品种为试材，采用SSR分子标记技术与毛细管电泳技术相结合的方法，研究了梨不同成熟期的种质资源遗传多样性，以期为梨栽培品种的品种鉴别、亲子关系鉴定、分类及系统关系研究、遗传图谱构建与标记辅助育种在分子水平上提供参考依据。结果表明：3份梨种质的观测等位基因数 (Na)平均为14.8，有效等位基因数 (Ne)平均为7.363 1，Shannon多样性指数 (I)平均为2.195，期望杂合度 (Nei)平均为0.845，说明梨总群体遗传变异偏高。依据梨系统将其分为白梨、秋子梨、日本梨、砂梨和西洋梨5个类群，其中白梨系统在Shannon多样性指数和期望杂合度上最高，分别为2.069 9和0.831 1，秋子梨系统最低，分别为0.420 8和0.303 6，白梨与砂梨类群的遗传一致度最高为0.715 2。
Abstract:
Sixty-three main varieties of Pyrus L.were used as experimental materials.The method of combination with SSR molecular marker technique and capillary electrophoresis technique was used to study the genetic diversity of Pyrus L.varieties in order to supply reference for breed identification，parent-child relationship identification，classification and systematic research，genetic map construction and marker-assisted breeding of Pyrus L.The results showed that，the average number of alleles (Na) was 7.363 1，the Shannon diversity index (I) was 2.195，the expected heterozygosity (Nei) average of 0.845，indicating that the total genetic variation of the pear population was high.According to the pear system，it was divided into five groups：white pear，autumn pear，Japanese pear，Chinese pear and European pear.The white pear system was the highest in Shannon diversity index and expected heterozygosity，which were 2.069 9 and 0.831 1,respectively.The lowest were respectively 0.420 8 and 0.303 6，white pear and sand pear group genetic consistency of the highest，was 0.715 2.

参考文献/References:

[1]蒲富慎,黄礼森,孙秉均,等.梨品种[M].北京:农业出版社,1989.

[2]林伯年,沈德绪.利用过氧化物同工酶分析梨属种质及亲缘关系[J].浙江农业大学学报,1983(9):235-242.
[3]邹乐敏,张西民,张志德,等.根据花粉形态探讨梨属植物的亲缘关系[J].园艺学报,1986(13):219-223.
[4]曲柏宏,严花淑,陈艳秋,等.同工酶分析在梨品种分类中的应用[J].延边大学农学学报,2003,25(1):86-91.
[5]杨槐俊.孢粉学在部分梨属植物分类研究中的应用[J].果树科学,1985(3):2-9.
[6]孔旭.中国果树栽培学[M].北京:农业出版社,1987:558-559.
[7]BAO L，CHEN K S，ZHANG D，et al.An assessment of genetic variability and relationships with in Asian pears based on AFLP(amplified fragment length polymorphism)markers[J].Hort Science,2008,116:374-380.[8]滕元文,鲍露,郑小艳.梨属植物的起源和系统关系[M]//赵尊练.园艺学进展(6).西安：陕西科学技术出版社,2004:23-29.
[9]BAO L,CHEN K S,ZHANG D,et al.Genetic diversity and similarity of pear(Pyrus L.) cultivars native to east Asia revealed by SSR(simple sequence repeat) markers[J].Genetic Sources and Crop Evolution,2007,54:959-971.
[10]田义柯,王彩虹,贾彦利,等.梨矮化基因pcDw的SSR标记定位[J].果树学报2008,25(3):404-407.
[11]KEVIN D A.Overview of capillary electrophoresis and capillary electrochromatography[J].J Chromatography，1999,856:443-463.
[12]孙毓庆,孙国祥,金郁.毛细管电泳指纹图谱及毛细管电泳质谱联用在中药质量控制中的作用[J].色谱,2008,26(2):160-165.
[13]高源,田路明,刘凤之,等.利用SSR荧光标记构建92个梨品种指纹图谱[J].园艺学报,2012,39(8):1437-1446.
[14]ROBINSON J P，HARRIS S A,JUNIPER B E.Taxonomy of the genus Malus Mill.(Rosaceae) with emphasis on the cultivated apple,Malus domesitca Borkh[J].Pl Syst Evol,2001,226:35-38.
[15]OHTA S，NISHITAN C，YAMAMOTO T.Chloroplast microsatellites in Prunus，Rosaceae[J].Mol Eeol Notes,2005（5）:837-840.
[16]王桂娥,晁群芳,梁建芳,等.改良CTAB法提取新疆一枝蒿干叶基因组DNA[J].中国实验方剂学杂志,2015(12):19-22.
[17]韦兴笃,胡学峰,陈容,等.10个良种梨的果实特征调查及品质评析[J].山西果树,2003(3):28.
[18]马兵钢,赵宗胜,冯建荣,等.梨属DNA提纯方法的比较研究[J].石河子大学学报(自然科学版),2000,4(4):277-281.
[19]KIMURA T,SHI Y Z,SHODA M,et al.Identifieation of Asian pear varieties by SSR analysis[J].Breeding Science,2002,52:115-121.
[20]LEE G P，LEE C H，KIM C S.Molecular markers derived from RAPD，SCAR and the conserved 18S rDNA sequences for classification and identification in P.pyrifolia and P.communis[J].Theor Appl Genet，2004，108:1487-1491.
[21]韩宏伟,杨敏生,徐兴兴,等.利用SSR标记鉴定主要梨栽培品种[J].中国农学通报,2006,22(12):383-386.
[22]程本义,施勇烽,沈伟峰,等.南方稻区国家水稻区域试验品种的微卫星标记分析[J].中国水稻科学,2007,21(1):7-12.
[23]程本义,施勇烽,沈伟峰,等.水稻品种DNA指纹检测技术体系及其应用[J].杂交水稻,2008,23(1):54-59.
[24]程本义,吴伟,夏俊辉,等.浙江省水稻品种DNA指纹数据库的初步构建及其应用[J].浙江农业学报,2009,21(6):555-560.
[25]PAN Y B，BURNER D M，LEGENDRE B L.An assessment of the phylogenetic relationship among sugarcane and related taxa based on the nucleotide sequence of 5S rRNA intergenic spacers[J].Genetica,2000,108:285-295.
[26]PAN Y B.Highly polymorphic microsatellite DNA markers for sugarcane germplasm evaluation and variety identity testing[J].Sugar Tech，2006（8）:246-256.
[27]PAN Y B,SCHEFFLER B E,RICHARD E P.High-through molecular genotyping of commercial sugarcane clone with microsatellite(SSR) markers[J].Sugar Tech，2007（9）:176-181.
[28]梁俊,PAN Y B,李杨瑞,等.利用SSR标记与毛细管电泳对甘蔗属进行的遗传分析[J].广西植物,2010,30(1):106-111.

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备注/Memo

第一作者简介：赵思思（1991-），女，河北石家庄人，硕士研究生，研究方向为林木遗传育种。E-mail：852729371@qq.com.

责任作者：梁海永（1973-），男，河北保定人，本科，副教授，现主要从事林业生物技术等研究工作。E-mail：lianghy@hebau.edu.cn.
基金项目：国家林业公益性行业资助项目（201104039）；国家林业局科技发展中心资助项目（XPC-201505）。
收稿日期：2017-04-06

更新日期/Last Update: 2017-08-29