[1]付金娥,黎颍菁,韦树根,等.叶片气孔保卫细胞周长用于鉴定黄花蒿倍性的研究[J].北方园艺,2017,41(06):156-161.[doi:10.11937/bfyy.201706037]
　FU Jine,LI Yingjing,WEI Shugen,et al.Leaf Stomatal Guard Cell Perimeter Used for Identification of Ploidy of Artemisia annua L.[J].Northern Horticulture,2017,41(06):156-161.[doi:10.11937/bfyy.201706037]
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叶片气孔保卫细胞周长用于鉴定黄花蒿倍性的研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年06 页码: 156-161 栏目: 出版日期: 2017-03-30
Title:
Leaf Stomatal Guard Cell Perimeter Used for Identification of  Ploidy of Artemisia annua L.
作者:
付金娥1; 黎颍菁2; 韦树根1; 潘丽梅1; 胡凤云1; 童婷婷1
（1.中国医学科学院 药用植物研究所广西分所，广西药用资源保护与遗传改良重点实验室，广西 南宁 530000； 2.广西绿色食品办公室，广西 南宁 530000）
Author(s):
FU Jin′e1; LI Yingjing2; WEI Shugen1; PAN Limei1; HU Fengyun1; TONG Tingting1
(1.Guangxi Branch of Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences/Guangxi Medicinal Resource Protection and Genetic Improvement Key Laboratory,Nanning,Guangxi 530000；2.Guangxi Green Food Office,Nanning,Guangxi 530000)
关键词:
黄花蒿; 气孔保卫细胞; 整体透明技术; 倍性鉴定
Keywords:
Artemisia annua L.; stomata guard cell; integral transparent technique; ploidy identification
DOI:
10.11937/bfyy.201706037
文献标志码:
A
摘要:
以二倍体、四倍体及经秋水仙素处理的黄花蒿植株为试材，采用整体透明技术制备黄花蒿叶片样品，对不同倍性植物的叶片气孔保卫细胞长轴长、短轴长及其周长进行考察，探讨气孔保卫细胞大小与植株倍性的相关性。结果表明：以10%氢氧化钾溶液为透明剂，透化时间为1.5 h的透化效果最好；二倍体与四倍体植物的气孔保卫细胞长轴长、短轴长及其周长差异显著，其周长范围分别为57.19～70.33 μm和83.36～91.99 μm；利用二倍体与四倍体的周长分布在不同区间范围，可以对秋水仙素处理的黄花蒿植株进行快速的初步倍性鉴定。
Abstract:
The diploid, tetraploid and colchicine treated Artemisia annua L. plants were used as the experimental materials. The integral transparent technique was applied to prepare the sample of the leave of Artemisia annua L. The long axis and short axis of stomatal guard cells of different ploidy plants were studied as well as the relationship between stomatal guard cell size and plant ploidy. The results showed that the optimal permeability was obtained by using 10% potassium hydroxide solution as the transparent agent and with the permeabilization time of 1.5 hours. There were significant difference between the length of long axis, short axis length and perimeter of stomatal guard cells of diploid and tetraploid plants, which perimeter range from 57.19-70.33 μm and 83.36-91.99 μm. The rapid preliminary ploidy identification of colchicine treated Artemisia annua L. plants was studies via the perimeter distribution in different interval range.

参考文献/References:

 

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相似文献/References:

[1]陈建中,葛水莲,焦云红,等.黄花蒿提取物在黄瓜保鲜中的应用研究[J].北方园艺,2013,37(01):169.
　CHEN Jian-zhong,GE Shui-lian,JIAO Yun-hong,et al.Applicaion of Extracts from Artemisia annua on Cucumber Reservation[J].Northern Horticulture,2013,37(06):169.

备注/Memo

第一作者简介：付金娥（1981-），女，安徽亳州人，硕士，副研究员，现主要从事中药材良种繁育及标准化等研究工作。E-mail：duanwei3014@163.com.责任作者：韦树根（1980-），男，广西灵山人，博士研究生，副研究员，现主要从事中药材良种繁育等研究工作。E-mail：weishugen2@163.com.基金项目：国家自然科学基金资助项目（81560623）；广西自然科学基金资助项目（2013GXNSFBA019180;2013GXNSFAA019221）。

更新日期/Last Update: 2017-04-05