[1]刘玉军,刘芳,孙灿岳,等.花叶良姜组培无菌体系的建立[J].北方园艺,2017,41(05):89-92.[doi:10.11937/bfyy.201705021]
LIU Yujun,LIU Fang,SUN Canyue,et al.Tissue Culture and Rapid Propagation of Alpinia zerumbet cv.Variegata[J].Northern Horticulture,2017,41(05):89-92.[doi:10.11937/bfyy.201705021]
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LIU Yujun,LIU Fang,SUN Canyue,et al.Tissue Culture and Rapid Propagation of Alpinia zerumbet cv.Variegata[J].Northern Horticulture,2017,41(05):89-92.[doi:10.11937/bfyy.201705021]
花叶良姜组培无菌体系的建立
《北方园艺》[ISSN:1001-0009/CN:23-1247/S]
卷:
第41卷
期数:
2017年05
页码:
89-92
栏目:
出版日期:
2017-03-15
- Title:
- Tissue Culture and Rapid Propagation of Alpinia zerumbet cv.Variegata
- 文献标志码:
- A
- 摘要:
- 以花叶良姜袋苗为试材，根茎腋芽为外植体，研究外植体预处理、灭菌消毒和外植体长度等因素对其无菌体系建立的影响。结果表明：实验室水培植株3 d，根茎腋芽长3～5 cm作外植体，用1.25‰ HgCl2灭菌消毒16 min，花叶良姜外植体污染率24.2%，萌芽率72.7%，较有利于建立花叶良姜无菌体系。
- Abstract:
- The pretreatment，sterilized factors and length of the explants were studied to establish propagation system on axillary buds of rhizome of Alpinia zerumbet cv.Variegata.The results showed that the contamination rate would be 24.2% and germination rate would be 72.7% when choose the axillary buds at the length of 3-5 cm from hydroponics plants for three days，the surface disinfection suitable time lasted 16 min in 1.25‰ HgCl2，and the growth of the explants was in good condition.
参考文献/References:
[1]赵秀芳.花叶艳山姜组培快繁技术研究[J].中国农学通报,2014,20(6):34-35,38. [2]梅贝坚,艾华.花叶良姜的组织培养[J].植物生理学通讯,1990(2):44-45. [3]德庆措姆,布穷.银白杨组织培养中外植体消毒时间初探[J].辽宁林业科技,2000(4):29-37. [4]ANDERSON M.The world encyclopedia of cacti & succulents[M].Sebastian Kelly,1999. [5]邢书慧,罗健,陈泳慧,等.通气对几种水培观赏植物生长的影响[J].农业工程学报,2005(21):36-40.
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MENG Qian,KONG Xiang-sheng,ZHANG Gai-na.Effect of Different Growth Regulators on Tissue Culture and Rapid Propagation of Salvia miltiorrhiza [J].Northern Horticulture,2013,37(05):102.
备注/Memo
第一作者简介：刘玉军（1969-），男，广西全州人，本科，林业工程师，现主要从事绿化苗木及珍贵树种苗木培育等研究工作。E-mail：598623826@qq.com.
更新日期/Last Update:
2017-03-24