LIU Guoqin,HE Songtao,LIU Jinping,et al.Changes of Some Physiological Indexes of Antioxidation Metabolism in Floral Buds of Peach During Dormancy[J].Northern Horticulture,2017,41(05):1-5.[doi:10.11937/bfyy.201705001]
桃花芽休眠期间部分抗氧化代谢生理指标的变化
- Title:
- Changes of Some Physiological Indexes of Antioxidation Metabolism in Floral Buds of Peach During Dormancy
- Keywords:
- Prunus persica L.var.densa Makino cv.‘Zhonghuashoutao’; floral bud; dormancy; antioxidation metabolism
- 文献标志码:
- A
- 摘要:
- 以‘中华寿桃’（Prunus persica L.var.densa Makino cv.‘Zhonghuashoutao’）为试材，连续2年研究其花芽在自然休眠过程中部分生理指标的变化规律。结果表明：‘中华寿桃’花芽在2013—2015年2年度间休眠进程、可溶性蛋白质含量、总氨基酸含量、过氧化物酶（POD）活性、过氧化氢（H2O2）含量和抗坏血酸（AsA）含量不同；2013—2014年度花芽内休眠维持时间比2014—2015年度长，2013—2014年度可溶性蛋白质含量高于2014—2015年度，而总氨基酸、POD活性、H2O2含量和AsA含量都低于2014—2015年度；2年度‘中华寿桃’花芽内休眠及其解除过程中花芽可溶性蛋白质含量逐渐降低，而总氨基酸含量则逐渐升高，POD活性和H2O2含量呈相似的变化趋势；AsA含量呈现“升-降-升-降”的变化规律，但最高值和最低值出现的时间在年度间有所不同。
- Abstract:
- The changes of some physiological indexes of antioxidation metabolism were studied in floral buds of ‘Zhonghuashoutao’ peach during the dormancy for two successive years.The results showed that there were some differences in dormancy progression，soluble protein content，total amino acids content，POD activity，H2O2 content，and AsA content in floral buds.The time of endodormancy maintenance of floral buds in 2013—2014 was longer than that of floral buds in 2014—2015.The content of soluble protein gradually decreased，however，the total amino acids content gradually increased during endodormancy and dormancy release.POD activity and H2O2 content occurred on the same change.It appeared the trend of variation ‘up-down-up-down’.But there was difference in the occurring date of maximum and minimum values of AsA content between two years.
参考文献/References:
[1]SAMISH R M.Dormancy in woody plants[J].Annual Review of Plant Physiology,1954,5(1):183-204. [2]高东升.果树芽休眠[M]//李天忠,张志宏.现代果树生物学.北京:科学出版社,2008:39-53. [3]LANG G A，EARLY J D，MARTIN G C，et al.Endo-，para-，and ecodormancy：physiological terminology and classification for dormancy research[J].Hort Science,1987,22(3):371-377. [4]COOKE J E K，ERIKSSON M E，JUNTTILA O，et al.The dynamic nature of bud dormancy in trees：environmental control and 〖JP3〗molecular mechanisms[J].Plant，Cell and Environment，2012，35(10):1707-1728. [5]高东升，束怀瑞，李宪利.几种落叶果树H2O2含量变化与自然休眠关系的研究[J].园艺学报,2002,29(3):209-213. [6]秦栋.酸王自然休眠解除后生理生化变化研究[D].泰安:山东农业大学,2009. [7]邵浩,马锋旺.梨树花芽休眠解除与活性氧代谢的关系[J].植物生理与分子生物学学报,2004，30(6):660-664. [8]张昂,郑瑜琬，陈腾,等.葡萄休眠解除过程中冬芽组织活性氧与抗氧化系统的变化特征[J].西北植物学报,2012(10):2075-2081. [9]LIU G Q，LI W S，ZHENG P H，et al.Transcriptomic analysis of ‘Suli’ pear (Pyrus pyrifolia white pear group) buds during the dormancy by RNA-Seq[J].BMC Genomics，2012,13(1):700.doi:101186/1471-2164-13-700. [10]刘国琴.梨休眠分子生理机制研究[D].杭州:浙江大学,2013. [11]刘国琴，郑鹏华，滕元文.落叶果树芽休眠相关基因的研究进展[J].果树学报,2012,29(5)：911-917. [12]谭钺，王茂生，吕勐，等.不同破眠处理对油桃休眠芽H2O2含量及相关酶活性的影响[J].中国农学通报,2014(28):128-132. [13]吴步科.桃休眠过程中活性氧代谢及其信号转导作用研究[D].兰州:甘肃农业大学,2006. [14]李波，夏秀英，刘思.蓝莓花芽休眠与解除过程中生理生化变化及DNA甲基化差异分析[J].植物生理学报,2015(7):1133-1141. [15]毕磊.七月酥梨休眠期内含物变化及生理代谢研究[D].保定:河北农业大学,2009. [16]杨艳，黎云祥，胥晓.珙桐种子休眠解除和萌发过程中主要抗氧化酶活性和代谢产物含量的变化[J].植物分类与资源学报,2015,37(6):779-787. [17]田莉莉,方金豹,王力荣,等.华光油桃解除休眠过程中几项生理指标的变化[J].果树学报,2006(1):121-124.
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备注/Memo
第一作者简介：刘国琴（1975-），女，博士，副教授，现主要从事植物生理与分子生物学等研究工作。E-mail：liuguoqin75@126.com.基金项目：国家自然科学基金资助项目（31560537）；贵州省科学技术基金资助项目（黔科合J[2015]2040号）；贵州大学引进人才科研资助项目（贵大人基合字（2013）24 号）。