[1]赵桐,周亦,唐云,等.白木香气生根细胞程序性死亡与2-(2-苯乙基)色酮关系[J].北方园艺,2017,41(20):157-162.[doi:10.11937/bfyy.20170169]
　ZHAO Tong,ZHOU Yi,TANG Yun,et al.Relationship Between PCD and Chromones Derivatives in Aerial Roots of Aquilaria sinensis[J].Northern Horticulture,2017,41(20):157-162.[doi:10.11937/bfyy.20170169]
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白木香气生根细胞程序性死亡与2-(2-苯乙基)色酮关系

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年20 页码: 157-162 栏目: 出版日期: 2017-10-30
Title:
Relationship Between PCD and Chromones Derivatives in Aerial Roots of Aquilaria sinensis
作者:
赵桐; 周亦; 唐云; 马新业; 詹若挺; 陈蔚文
广州中医药大学 中药资源科学与工程研究中心，岭南中药资源教育部重点实验室，国家中成药工程技术研究中心南药研发实验室，广东 广州 510006
Author(s):
ZHAO Tong; ZHOU Yi; TANG Yun; MA Xinye; ZHAN Ruoting; CHEN Weiwen
Research Center of Chinese Herbal Resource Science and Engineering,Guangzhou University of Traditional Chinese Medicine/Key Laboratory of Chinese Medicinal Resource from Lingnan/Ministry of EducationJoint Laboratory of National Engineering Research Center for the Pharmaceutics of Traditional Chinese Medicines,Guangzhou,Guangdong 510006
关键词:
白木香; 气生根; 细胞程序性死亡; 2-(2-苯乙基）色酮
Keywords:
Aquilaria sinensis; aerial roots; programmed cell death (PCD); 2-(2-phenylethyl) chromone
DOI:
10.11937/bfyy.20170169
文献标志码:
A
摘要:
以白木香气生根为试材，采用50 μmol?L-1茉莉酸甲酯(MeJA)分别处理0(CK)、3、5、7 d后，通过显微观察、TUNEL检测、DNA降解和GC/MS检测方法，探讨MeJA对程序性细胞死亡(PCD）和沉香色酮产生之间的关系。结果表明：MeJA处理5、7 d后检测到PCD的发生，同时通过GC/MS检测到2-(2-苯乙基）色酮的产生且相对含量逐渐增加。推测白木香气生根在MeJA处理后，PCD的发生可能与2-(2-苯乙基）色酮的产生存在正相关性，为进一步研究沉香色酮的形成机制提供参考依据。
Abstract:
Aerial roots of Aquilaria sinensis were used as experimental material，the effects of MeJA on programmed cell death (PCD) and the production of aloes ketones were studied by 50 μmol?L-1 methyl jasmonate (MeJA) treatment for 0，3，5，7 days respectively，and the materials were observed and detected by microscopic observation，TUNEL detection，DNA degradation and GC/MS method respectively.The results showed that the occurrence of PCD was detected in the experimental material that had been processed by MeJA for 5 days and 7 days，at the same time，the production of 2-(2-phenylethyl)chromone was detected by GC/MS method and the relative content was increased gradually.This study speculated that the occurrence of PCD might be positively correlated with the production of 2-(2-phenylethyl)chromone after MeJA treatment，which could provide reference for the further study of the formation mechanism of the agarwood chromone.

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备注/Memo

第一作者简介：赵桐(1989-），男，硕士，现主要从事中药资源利用与开发等研究工作。E-mail：13535458015@163.com.

责任作者：马新业(1976-），男，博士，副研究员，硕士生导师，现主要从事中药资源学等研究工作。E-mail：usermxy@163.com.
基金项目：国家自然科学基金资助项目(81102764）；广东省大学学科和专业建设专项基金资助项目(2013CXZDA011）；广东省普通高等教育创新团队—传统中药资源创新研究资助项目(2016KYTD02）。
收稿日期：2017-04-05

更新日期/Last Update: 2017-11-02