[1]王耘,金潇潇,赵辉,等.盐胁迫对鲜食蚕豆幼苗光合特性及抗氧化酶活性的影响[J].北方园艺,2017,41(20):25-30.[doi:10.11937/bfyy.20170164]
　WANG Yun,JIN Xiaoxiao,ZHAO Hui,et al.Effect of Salt Stress on Photosynthesis and Antioxidant Enzyme Activities of Vicia faba L.Seedling[J].Northern Horticulture,2017,41(20):25-30.[doi:10.11937/bfyy.20170164]
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盐胁迫对鲜食蚕豆幼苗光合特性及抗氧化酶活性的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年20 页码: 25-30 栏目: 出版日期: 2017-10-30
Title:
Effect of Salt Stress on Photosynthesis and Antioxidant Enzyme Activities of Vicia faba L.Seedling
作者:
王耘; 金潇潇; 赵辉; 郝振萍
金陵科技学院 园艺学院，江苏 南京 210038
Author(s):
WANG Yun; JIN Xiaoxiao; ZHAO Hui; HAO Zhenping
College of Horticulture,Jinling Institute of Technology,Nanjing,Jiangsu 210038
关键词:
盐胁迫; 鲜食蚕豆; 光合特性; 抗氧化酶
Keywords:
salt stress; Vicia faba L.; photosynthesis; antioxidant enzyme
DOI:
10.11937/bfyy.20170164
文献标志码:
A
摘要:
以鲜食蚕豆(Vicia faba L.）品种“通蚕鲜6号”为试材，采用1/2 Hoagland营养液栽培，研究不同浓度NaCl(0、50、100、150 mmol?L-1）处理对鲜食蚕豆幼苗生长、光合特性和抗氧化酶等生理代谢的影响，为盐碱地区蚕豆的种植提供参考依据。结果表明：盐处理时，蚕豆生长呈现低浓度(50 mmol?L-1）促进，中高浓度(≥100 mmol?L-1）抑制，尤其是高浓度处理组植株干质量比对照组下降了64.3%，并且在高浓度时蚕豆净光合速率、蒸腾速率、气孔导度和胞间CO2 浓度也均呈降低趋势，分别下降了19.7%、56.3%、74.0%和61.2%；但是叶绿素a、叶绿素b和叶绿素(a+b）的含量在3种盐浓度处理时均出现降低趋势；在低中浓度处理时，超氧化物歧化酶(SOD）活性升高，高浓度处理时，胁迫程度加深，酶活性大幅度下降；过氧化物酶(POD）随着盐浓度升高和时间的延长均呈现升高趋势；过氧化氢酶(CAT）在低中浓度时有上升现象，低浓度上升幅度更大，在高浓度时呈先上升后下降的趋势；3个处理组的MDA含量均随盐浓度升高呈现增长趋势。
Abstract:
Vicia faba L.was used as material,the treatment was applied via soilless substrate′s pot experiment.The effect of salt stress on growth and physiology characteristics were studied.The experiment was carried out using ‘No.6 Tongcanxian’ to explore effects druing 12 days of NaCl stress (0,50,100,150 mmol?L-1) on biomass,net photosynthetic rate (Pn),stomatal conductivity (Gs),intercellular CO2 concentration (Ci),transpiration rate (Tr),chlorophyll (Chl),the activity of antioxidase (SOD,POD,CAT) and the MDA content.The results showed that NaCl stress increased plant dry weight at low salt concentration,while at high concentration the dry weight decreased 64.3% and Pn,Tr,Gs and Ci in seedling were decreased 19.7%,56.3%,74.0% and 61.2%,respectively.Comparing with the control group,the chlorophyll (Chl a and Chl b) content and Chl(a+b) content both displayed descending.Under the lower NaCl concentration,the activity of SOD increased,on the other hand,under the high concentration the activity decreased.The activity of POD content increased with the increasing of NaCl concentration and time.The CAT activity was the highest when salt concentration was 150 mmol?L-1 on the 9th day,then decreased.With increasing stress degree the MDA content increased.Growth and index of physiology could all be regarded as indexes for evaluation of Vicia faba L.in salt adaptability.

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备注/Memo

第一作者简介：王耘(1985-），女，江苏南京人，博士，讲师，现主要从事园艺作物品质及生理等研究工作。E-mail：wangyun@jit.edu.cn.

责任作者：郝振萍(1981-），女，硕士，讲师，现主要从事植物生理等研究工作。E-mail：yyxhao@jit.edu.cn.
基金项目：江苏省高校自然科学研究资助项目(13KJD210001）；江苏省自然科学基金青年基金资助项目(BK20130093）。
收稿日期：2017-07-04

更新日期/Last Update: 2017-10-31