YIN Mingzhi,HU Yan.Cloning and Bioinformatics Analysis of DOF Protein Gene in Brassica napus L.[J].Northern Horticulture,2017,41(14):18-24.[doi:10.11937/bfyy.20170162]
甘蓝型油菜DOF蛋白基因的克隆与生物信息学分析
- Title:
- Cloning and Bioinformatics Analysis of DOF Protein Gene in Brassica napus L.
- Keywords:
- Brassica napus L.; Dof protein gene; silico cloning; bioinformatics
- 文献标志码:
- A
- 摘要:
- Dof转录因子是植物特有的一类转录因子，含有一个独特的单锌指结构域，在植物的生长发育中起重要的调控作用。以甘蓝型油菜为试材，采用电子克隆和RT-PCR技术，〖JP+2〗以期克隆一个具有Zf-Dof结构域的蛋白基因cDNA序列。结果表明：该基因含有〖JP〗1 284 bp的开放阅读框，编码427个氨基酸，蛋白分子量为46.9 kDa，等电点为8.86，为亲水性蛋白。经序列比对分析表明其与拟南芥CDF3基因有较高同源性，可能具有相似的功能。
- Abstract:
- Dof transcription factor is a kind of plant specific transcription factor，which contains an unique single zinc finger domain，and plays an important role in the growth and development of plants.In this study，a cDNA gene sequence with Zf-Dof domain was cloned by silico cloning and RT-PCR in Brassica napus.The results showed that the gene was a hydrophilic protein,containing an open reading frame of 1 284 bp，encoding a total of 427 amino acids with a molecular weight of 46.9 kDa and an isoelectric point of 8.86.Sequence alignment analysis showed that it had high homology with Arabidopsis CDF3 gene，which might have similar functions.
参考文献/References:
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备注/Memo
第一作者简介：尹明智（1983-），男，博士，副教授，现主要从事油菜遗传育种等研究工作。E-mail：277536002@qq.com.基金项目：国家自然科学基金资助项目（31501337）；贵州省科学技术厅与遵义市科学技术局及遵义师范学院联合科技基金资助项目（黔科合LH字[2016]7014号）；遵义师范学院博士基金资助项目（遵师BS[2014]25号）。