ZHU Huanhuan,JIN Yingling,CHENG Yongan,et al.Inhibition Effect of Powdery Mildew Development by Foliar Spray Four Inducers to Summer Squash (Cucurbita pepo L.)[J].Northern Horticulture,2017,41(01):118-125.[doi:10.11937/bfyy.201701026]
叶面喷施四种诱导剂预防西葫芦白粉病效果
- Title:
- Inhibition Effect of Powdery Mildew Development by Foliar Spray Four Inducers to Summer Squash (Cucurbita pepo L.)
- Keywords:
- summer squash(Cucurbita pepo L.); powdery mildew; dipotassium phosphate; prevention efficacy
- 文献标志码:
- A
- 摘要:
- 以3个西葫芦品种和6份西葫芦种质为试材，通过叶面喷施5 g?L-1 NaHCO3、5.706 g?L-1 K2HPO4、2.62 g?L-1 K2SiO3 和RM（0.188 g?L-1核黄素+0.746 g?L-1蛋氨酸），研究了4种药剂对西葫芦白粉病的预防效果及对单株产量和叶片中可溶性糖及可溶性蛋白质含量的影响，以期探寻预防西葫芦白粉病的化学农药替代技术。结果表明:对西葫芦白粉病的预防效果表现为5.706 g?L-1 K2HPO4＞5 g?L-1 NaHCO3＞2.62 g?L-1 K2SiO3＞RM；4种诱导剂处理均能在一定程度上有效延缓西葫芦白粉病的发展进程，且有促进植株生长的作用；各处理对西葫芦单株产量的影响没有明显的规律，但产量均较对照有所提高，以5.706 g?L-1 K2HPO4和 RM处理的增产效果较好。植株诱导抗性增强与产量增加呈正相关，相关系数为0.693；各处理均提高了西葫芦植株叶片中的可溶性糖和可溶性蛋白质含量，植株诱导抗性增强与可溶性糖含量增加呈正相关，相关系数为0.754。表明西葫芦可能通过调节植株内源营养物质的种类及含量来调节自身应激反应，诱导形成对白粉病的抗病机制。
- Abstract:
- Three varieties of summer squash and six pieces of germplasm were used as test materials.The inhibition effects of four inducers(5 g?L-1 NaHCO3，5.706 g?L-1 K2HPO4，2.62 g?L-1 K2SiO3 and RM(0.188 g?L-1 riboflavin+0.746 g?L-1 methionine) on powdery mildew，yield，soluble sugar content and soluble protein content were studied to search alternative technologies of chemical pesticide to prevent squash powdery mildew.The results showed that the inhibition effect on powdery mildew of summer squash was 5.706 g?L-1 K2HPO4＞5 g?L-1 NaHCO3＞2.62 g?L-1 K2SiO3＞RM.To a certain degree,the four kinds of inducer treatments could postpone effectively powdery mildew occurrence and promote plant growth.The effect on individual plant yield of summer squash had no obvious regularity，but all were higher than control.The effect of 5.706 g?L-1 K2HPO4 and RM treatment were better.Plant added yield was positively correlated to increase resistance，the correlation coefficient was 0.693.The soluble protein content and soluble sugar content of summer squash leaf increased after treatments.Soluble sugar content increase was positively correlated to increasing resistance，the correlation coefficient was 0.754.The measures induced the summer squash own stress reaction and formation of powdery mildew resistant mechanism by adjusting the endogenous type and content of nutrients.
参考文献/References:
[1]孙茜.西葫芦南瓜疑难杂症图片对比诊断与处方[M].北京:中国农业出版社，2010:34-37. [2]咸丰，张勇,马建祥,等.陕西关中地区瓜类白粉病菌生理小种的鉴定[J].西北农林科技大学学报(自然科学版)，2010,38(10):115-120. [3]张海霞.浅谈植物诱导抗病性的研究进展[J].科技与企业，2012(17):305. [4]HOMMA Y，ARIMOTO Y，MISATO T.Effect of sodium bicarbonate on each growth stage of cucumber powdery mildew fungus (Sphaerotheca fuliginea) in its life cycle[J].Pestic Sci，1981（6）:201-209. [5]HORST R K，KAWAMOTO S O,PORTER L L.Effect of sodium bicarbonate and oils on the control of powdery mildew and black spot of roses[J].Plant Dis，1992,76：247-251. [6]ZIV O，ZITTER T A.Effect of bicarbonates and film-forming polymers on cucurbit foliar diseases[J].Plant Dis，1992，76:513-517. [7]INOUE S，MACKO V，AIST J R.Identification of the active component in the papilla-regulating extract from barley leaves[J].Physiol Mol Plant Pathol，1994,44：441-453. [8]REUVENI,AGAPOV V.Controlling powdery mildew caused by Sphaerotheca fuliginea in cucumber by foliar sprays of phosphate and potassium salts[J].Crop Prorecrron，1996,15(1)：49-53. [9]朱振家,安翠香,马育斌,等.几种化学诱导物对甜瓜白粉病抗性的诱导作用[J].甘肃农业大学学报，2007,42(5)：100-103. [10]CARVER T L W，ZEYEN R J，AHLSTRAND G G.The relation between insoluble silicon and success or failure of attempted penetration by powdery mildew (Erysiphe graminis) germlings on barley[J].Physiological Plant Pathology，1987,31:133-148. [11]MENZIES J G，EHRET D L，GLASS A D M.Effects of soluble silicon on the parasitic fitness of Sphaerotheca fuliginea on cucumber is sativus[J].Phytopathology，1991,4(24)：84-88. [12]MENZIES J G，BOWEN P，EHRET D L,et al.Foliar applications of potas-siumsilicate reduce severity of powdery mildew on cucumber，muskmelon，and zucchini squash[J].Am Soc Hort Sci，1992,117：902-905. [13]魏国强,朱祝军,钱琼秋,等.硅对黄瓜白粉病抗性的影响及其生理机制[J].植物营养与肥料学报,2004,10(2):202-205. [14]肖卫华,李里特,王慧敏.电生功能水防治黄瓜白粉病试验初报[J].植物保护，2003,29(2)：50-51. [15]韩欢欢.西葫芦种株白粉病防控及抗性诱导[D].泰安:山东农业大学,2013. [16]JIA C，DAI G H.Effect of dpinitol isolated and identified from Robinia pseudoacacia against cucumber powdery mildew[J].Scientia Horticulturae，2014,176：38-44. [17]程伯瑛,李海真,贾长才,等.西葫芦白粉病发展动态及药剂防治技术研究[J].中国蔬菜，2005(12)：35-36. [18]BRADFORD M.A rapid and sensitive method the quantitation of microgram quantities of prote in utilizing the principle of protein-dye binding[J].Analytical Biochemistry，1976,72:248-254. [19]张志良.植物生理学实验指导[M].北京：高等教育出版社,2002:127-128,159-160. [20]刘海清,马保国,孙红.施用硅肥对黄瓜抗白粉病及其产量的影响[J].河南农业学报,2005(5):65-66. [21]李洪连,王守正,王金生,等.黄瓜对炭疽病诱导抗性的初步研究[J].植物病理学报，1993,23(4)：327-332. [22]宋凤鸣.氟乐灵诱发棉苗对枯萎病的诱导抗性机制[J].植物病理学报，1993，23(2)：114-117. [23]冯杰.棉株体内几种生化物质与抗枯萎病之间的关系的初步研究[J].植物病理学报，1991,21(4):291-297. [24]杨家书.植物苯丙氨酸类代谢与小麦对白粉病抗性的关系[J].植物病理学报，1986,16(3):169-173. [25]李靖.黄瓜感染霜霉病菌叶片中一些酶活性的变化[J].植物病理学报,1991,21(4)：277-283.
相似文献/References:
[1]杨 爽,李海鹏,杨培鑑,等.不同草莓品种对白粉病的抗性调查[J].北方园艺,2014,38(10):104.
YANG Shuang,LI Hai-peng,YANG Pei-jian,et al.Survey on the Resistance to the Powdery Mildew of Different Strawberries Cultivars[J].Northern Horticulture,2014,38(01):104.
[2]潘春清,高红秀,高黎力,等.西葫芦褐腐病的发生特点及防治方法[J].北方园艺,2014,38(10):107.
PAN Chun-qing,GAO Hong-xiu,GAO Li-li,et al.Features Occurred and Control Methods of Summer Squash Brown Rot[J].Northern Horticulture,2014,38(01):107.
[3]刘凤兰,杜新民,吴忠红,等.水杨酸浸种对西葫芦幼苗生长及抗氧化酶活性的影响[J].北方园艺,2013,37(13):1.
LIU Feng-lan,DU Xin-min,WU Zhong-hong,et al.Effect of Soaking Seed with SA on Growth and the Activity of Antioxidant Enzymes of Cucurbita pepo L.Seedling[J].Northern Horticulture,2013,37(01):1.
[4]刘文君,王日升,黄凤婵,等.密本南瓜白粉病抗性遗传规律分析[J].北方园艺,2013,37(14):31.
LIU Wen-jun,WANG Ri-sheng,HUANG Feng-chan,et al.Inheritance Analysis of Powedery Mildew Resistance in Pumpkin[J].Northern Horticulture,2013,37(01):31.
[5]杜学林,任爱芝,邢光耀,等.植物油乳油对白粉病分生孢子细胞壁作用机理研究[J].北方园艺,2013,37(11):119.
DU Xue-lin,REN Ai-zhi,XING Guang-yao,et al.Study on Mechanism of Vegetable Oil EC on the Spores Cell Wall of Powdery Mildew[J].Northern Horticulture,2013,37(01):119.
[6]莱芜职业技术学院 冶金与建筑工程系,山东 莱芜 700.西葫芦银叶病对西葫芦品质的影响[J].北方园艺,2013,37(05):114.
HAO Shu-qin,SUI Jing,ZHENG Wei.Effect of Bemisia argentifolii on the Fruit Quality of Culurbita pepo L.[J].Northern Horticulture,2013,37(01):114.
[7]周梦韩,汪静,孟新刚,等.感染白粉病菌初期黄瓜叶片生理指标的变化[J].北方园艺,2013,37(03):133.
ZHOU Meng-han,WANG Jing,MENG Xin-gang,et al.Physiological Indexes Changes of Cucumber after Infected by Sphaerotheca fuliginea at the Beginning Period[J].Northern Horticulture,2013,37(01):133.
[8]纪 程,邱显钦,张 颢,等.中国古老月季资源的白粉病抗性鉴定[J].北方园艺,2013,37(09):146.
JI Cheng,QIU Xian-qin,ZHANG Hao,et al.Powdery Mildew Resistance Identification of Chinese Old Garden Roses[J].Northern Horticulture,2013,37(01):146.
[9]陈凤真.西葫芦果实颜色遗传研究[J].北方园艺,2013,37(19):19.
CHEN Feng-zhen.Study on the Heredity of Fruit Color of Summer Squash[J].Northern Horticulture,2013,37(01):19.
[10]刘子记,刘昭华,牛玉,等.不同苦瓜材料农艺性状调查及白粉病抗性分析[J].北方园艺,2013,37(19):117.
LIU Zi-ji,LIU Zhao-hua,NIU Yu,et al.Agronomic Traits Investigation and Powdery Mildew Resistance Evaluation of Bitter Gourd Germplasm[J].Northern Horticulture,2013,37(01):117.
备注/Memo
第一作者简介：朱焕焕（1990-），女，硕士研究生，研究方向为蔬菜育种与生物技术。E-mail：1154909220@qq.com.责任作者：张明科（1970-）,男，博士，副研究员，研究方向为蔬菜育种与生物技术。E-mail：zhangmk1101@nwsuaf.edu.cn.基金项目：公益性行业（农业）科研专项资助项目（201303112）；国家大宗蔬菜产业技术体系建设资助项目（CARS-25）。