QI Yawen,WANG Rong,ZHAO Qiulin,et al.Study on Content Determination of 6-ketone Steroidal Saponins From Smilax scobinicaulis[J].Northern Horticulture,2017,41(01):107-113.[doi:10.11937/bfyy.201701024]
黑刺菝葜中6-酮型甾体皂苷含量测定方法
- Title:
- Study on Content Determination of 6-ketone Steroidal Saponins From Smilax scobinicaulis
- 关键词:
- 黑刺菝葜; 6-酮型甾体皂苷; 紫外可见分光光度法; 星点设计-响应面法
- Keywords:
- Smilax scobinicaulis C.H.Wright; 6-ketone type steroidal saponins; UV-VIS spectrophotometry; CCD-RSM
- 文献标志码:
- A
- 摘要:
- 以黑刺菝葜为试材，应用紫外可见分光光度法，研究测定其6-酮型甾体皂苷类化合物含量的方法；以LG-C为标准品，依据显色剂对LG-C反应的专属性和灵敏度，确定紫外可见分光光度法的显色体系和最大吸收波长；在单因素试验的基础上，通过星点设计-响应面法，确定最适显色反应条件。结果表明：紫外可见分光光度法的显色体系为茴香醛-硫酸法Ⅱ，最大吸收波长为429 nm；最佳显色温度82.4 ℃，反应时间24 min，显色剂用量1.2 mL。优化反应条件后，在7.51~50.90 μg范围内，LG-C与吸光度值呈良好线性关系，r =0.996 9；平均加样回收率为103.37%，RSD 2.2%。该方法灵敏度高、专属性强，重复性好，可以作为黑刺菝葜中6-酮型甾体皂苷含量的测定方法。
- Abstract:
- To develop a method for the determination the contents of 6-ketone steroidal saponins from Smilax scobinicaulis C.H.Wright，using LG-C as standard substance，different chromogenic agents，the optimal UV-VIS spectrophotometry and the optimize the chromogenic condition were studied.The results showed that the proposed method was anisaldehyde sulphuric acid Ⅱ in the circumstances，and，the maximum absorption wavelength was 429 nm.The optimal temperature was 82.4 ℃，the best reaction time was 24 min，and 1.2 mL chromogenic agent was fitted.The calibration curve of LG-C showed good linearity in the range of 7.51-50.90 μg，with r=0.996 9.The recovery was detected to be 103.37%，with RSD being 2.2%，respectively.The established colorimetric method provided sensitive，specific and accurate for determination of 6-ketone type steroidal saponins in Smilax scobinicaulis C.H.Wright.
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备注/Memo
第一作者简介：祁雅雯（1991-），女，硕士研究生，研究方向为天然产物化学及其资源利用。E-mail：yawen_qi@nwsuaf.edu.cn.责任作者：张存莉（1967-），女，博士，教授，博士生导师，现主要从事天然产物化学和植物资源利用的教学与科研工作。E-mail：cunli_zhang@nwsuaf.edu.cn.基金项目：国家863项目子课题资助项目（2013AA102902-8）。