WANG Minle,LI Shoumian,LI Ming,et al.Screening of Parental Strains of Pleurotus eryngii Quel[J].Northern Horticulture,2017,41(15):145-150.[doi:10.11937/bfyy.20170013]
工厂化杏鲍菇杂交亲本筛选
- Title:
- Screening of Parental Strains of Pleurotus eryngii Quel
- Keywords:
- Pleurotus eryngii Quel; factory production; parents screening; ISSR
- 文献标志码:
- A
- 摘要:
- 以国内引进的12个杏鲍菇菌株(P1~P12)为试材,采用ISSR分子标记方法分析菌株间的遗传距离,研究了不同菌株的菌丝体阶段和子实体阶段的差异。结果表明:P4菌株产量高、生物学效率高,P9菌株生长周期短、菌柄长,适宜作为杂交亲本。
- Abstract:
- Twelve strains of Pleurotus eryngii Quel were used as test materials.These strains were numbered as P1-P12.The genetic distance between strains was studied by ISSR molecular marker method,and differences in mycelial and fruiting stages of the twelve strain were analyzed.The results showed that the yield of P4 strain was high and the biological efficiency was high;the growth period of P9 strain was short and the stipe length was long.P4 strain and P9 strain were used as hybrid parents in this experiment.
参考文献/References:
[1]郑雪平,冀宏,尹永刚,等.中国杏鲍菇工厂化生产实践及问题分析与展望[J].食用菌,2014(1):7. [2]田景花,李明,李守勉.我国杏鲍菇生产研究进展[J].北方园艺,2013(4):179-181. [3]王瑞娟,郭力刚,刘朝贵,等.工厂化栽培杏鲍菇优良菌株筛选[J].食用菌学报,2006,13(3):19-21. [4]曲绍轩,高山,黄晨阳.SRAP、ISSR和RAPD分子标记技术在银耳菌株鉴别上的应用[J].食用菌学报,2007,14(3):1-5. [5]刘宇,陈文良.杏鲍菇13号杂交菌株选育研究[J].食用菌学报,2004,11(3):61-64. [6]李月梅,采俊香,牛瑞青.不同基质配方工厂化栽培杏鲍菇研究[J].北方园艺,2012(7):177-179. [7]徐全飞,郭亮,充娜,等.杏鲍菇工厂化栽培优良菌株的筛选及亲缘关系评价[J].食用菌学报,2010,17(2):19-21. [8]董伟,陶鸿,卢伟,等.10个杏鲍菇菌株的比较研究[J].中国食用菌,2010,29(3):26-28. [9]李辉平,宋金俤,林金盛,等.主要环境因子对杏鲍菇工厂化生产影响的研究[J].江西农业学报,2011,23(11):28-30. [10]黄志龙,肖淑霞,上官舟建.杏鲍菇优良菌株筛选及配套标准栽培技术[J].食用菌,2008(2):25-27. [11]陆娜,周祖法,王伟科,等.利用ISSR分子标记鉴别杏鲍菇生产菌株的研究[J].北方园艺,2015(21):150-152.
相似文献/References:
[1]田景花,李明,李守勉,等.我国杏鲍菇生产研究进展[J].北方园艺,2013,37(04):179.
[2]黄 亮,王 玉,班立桐,等.杏鲍菇枝条栽培种的应用及效果研究[J].北方园艺,2014,38(12):122.
HUANG Liang,WANG Yu,BAN Li-tong,et al.Study on the Application of the Branch Strain for Pleurotus eryngii Cultivar[J].Northern Horticulture,2014,38(15):122.
[3]刘达玉,王慧超,郑林用,等.补肾益寿胶囊药渣栽培杏鲍菇和姬菇的研究[J].北方园艺,2014,38(13):145.
LIU Da-yu,WANG Hui-chao,ZHENG Lin-yong,et al.Cultivation of Pleurotus eryngii and Pleurotus cornucopiae Using Bushen Yishou Capsule Residues as Substrate[J].Northern Horticulture,2014,38(15):145.
[4]林群英,吴亮亮,张锋伦,等.氮源对金针菇菌糠栽培杏鲍菇的改良作用研究[J].北方园艺,2013,37(22):151.
LIN Qun-ying,WU Liang-liang,ZHANG Feng-lun,et al.Study on the Modified Effect of Nitrogen Source on Culture of Pleurotus eryngii with Mushroom Bran of Flammulina velutipes [J].Northern Horticulture,2013,37(15):151.
[5]李守勉,李 明,田景花.不同培养时间对杏鲍菇酯酶同工酶酶谱的影响[J].北方园艺,2014,38(14):98.
LI Shou-mian,LI Ming,TIAN Jing-hua.Influences of Different Culture Times on Zymogram of Esterase Isozyme of Pleurotus eryngii[J].Northern Horticulture,2014,38(15):98.
[6]郝涤非.杏鲍菇原种及栽培种培养试验[J].北方园艺,2014,38(14):149.
HAO Di-fei.Culture Experiments of Original Species and Cultivars of Pleurotus eryngii [J].Northern Horticulture,2014,38(15):149.
[7]王迎鑫,郭倩,刘朝贵,等.真姬菇工厂化生产配方筛选研究[J].北方园艺,2014,38(04):129.
WANG Ying-xin,GUO Qian,LIU Chao-gui,et al.Research on Formulation Optimization of Hypsizygus marmoreusFactory Production[J].Northern Horticulture,2014,38(15):129.
[8]李富利,李富花.玉米芯栽培杏鲍菇品种比较试验[J].北方园艺,2014,38(05):132.
LI Fu-li,LI Fu-hua.Comparison Test on Corncob Cultivation of Pleurotus eryngii[J].Northern Horticulture,2014,38(15):132.
[9]杨丽维,王玉,班立桐,等.杏鲍菇液体菌种培养基的筛选和优化[J].北方园艺,2014,38(06):150.
YANG Li-wei,WANG Yu,BAN Li-tong,et al.Study on the Screening and Optimizing of Liquid Spawn Medium of Pleurotus eryngii[J].Northern Horticulture,2014,38(15):150.
[10]闫永亮,张秀云.生姜秸秆栽培杏鲍菇培养基优化研究[J].北方园艺,2014,38(07):140.
YAN Yong-liang,ZHANG Xiu-yun.Study on Optimization of Formula for Cultivation of Pleurotus eryngii Using Ginger Straw[J].Northern Horticulture,2014,38(15):140.
备注/Memo
第一作者简介:王民乐(1990-),男,硕士研究生,研究方向为食用菌生物技术与遗传育种。E-mail:574487113@qq.com.责任作者:李守勉(1978-),女,硕士,副教授,硕士生导师,现主要从事食用菌栽培生理与遗传育种及设施蔬菜栽培生理等研究工作。E-mail:yylsm@hebau.edu.cn.基金项目:河北省科技支撑计划资助项目(15222910D);河北省现代农业产业技术体系食用菌创新团队资助项目(HBCT2013060203)。收稿日期:2017-03-02