SUN Xiaoli,ZHANG Xinrong,TIAN Shoule,et al.Effect of Exogenous Hydrogen Sulfide on Resistance of Drought Stress of Chestnut Seedlings[J].Northern Horticulture,2017,41(15):7-12.[doi:10.11937/bfyy.20165060]
外源硫化氢处理对板栗幼苗干旱胁迫抗性的影响
- Title:
- Effect of Exogenous Hydrogen Sulfide on Resistance of Drought Stress of Chestnut Seedlings
- 关键词:
- 板栗; 硫氢化钠（NaHS）; 干旱胁迫
- Keywords:
- chesnut; NaHS; drought stress
- 文献标志码:
- A
- 摘要:
- 以“黄棚”板栗当年生幼苗为试材，通过水培的方法，研究了15% PEG胁迫下添加0.5、1.0 mmol?L-1硫氢化钠（NaHS）对板栗幼苗叶绿素含量、丙二醛(MDA)含量、脯氨酸(Pro)含量及抗氧化酶活性的影响，以研究外源硫化氢调节板栗的抗旱性机制。结果表明：干旱胁迫下，外施NaHS能显著降低板栗幼苗叶片MDA含量，提高叶绿素含量及脯氨酸含量，板栗幼苗叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性显著提高，有效地降低了干旱胁迫下细胞膜受损程度，提高了板栗对干旱逆境的适应性，其中0.5 mmol?L-1 NaHS浓度处理效果更好。
- Abstract:
- One-year-old seedling of chestnut ‘Huangpeng’was used as test material，a hydroponic experiment was carried out to study the effects of exogenous hydrogen sulfide at the concentration of 0.5 mmol?L-1 and 1.0 mmol?L-1 on the chlorophyll content under 15%PEG stress，malondialdehyde(MDA)，proline(Pro) and the activities of antioxidant enzymes of chesnut seedlings were studied，to investigate the related mechanisms of exogenous hydrogen sulfide regulation of drought stress of chesnut.The results showed that under drought stress spraying exogenous hydrogen sulfide obviously reduced the content of MDA，and significantly increased the contents of chlorophyll and proline，and the activities of SOD，POD，CAT.The previous evidence showed that exogenous hydrogen sulfide was favorable to decrease the damage degree of cell membrane under drought stress，which enhance the ability of resisting drought stress in chenut leaves.0.5 mmol?L-1 NaHS concentration was better.
参考文献/References:
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备注/Memo
第一作者简介：孙晓莉（1986-），女，硕士，助理研究员，现主要从事板栗栽培育种等研究工作。E-mail:runxiaoli@163.com.责任作者：沈广宁（1979-），男，硕士，副研究员，现主要从事板栗育种等研究工作。E-mail：gnshens@163.com.基金项目：山东省农业科学院青年科研基金资助项目（2016YQN29）；国家自然科学基金资助项目（31301733，31501742）；国家科技支撑计划课题资助项目(2013BAD14B04)。 收稿日期：2017-04-11