LYU Miaomiao,NA Ying,SUN Mudi,et al.Natural Incidence Investigation and RT-PCR Detection of Wine Grapes Virus Disease in Helan Mountain East Region of Ningxia[J].Northern Horticulture,2017,41(13):55-62.[doi:10.11937/bfyy.20164951]
宁夏贺兰山东麓酿酒葡萄病毒病田间自然发病率调查及检测
- Title:
- Natural Incidence Investigation and RT-PCR Detection of Wine Grapes Virus Disease in Helan Mountain East Region of Ningxia
- 关键词:
- 宁夏贺兰山东麓; 酿酒葡萄病毒病; 田间自然发病率; 葡萄卷叶伴随病毒（GLRaVs）; RT-PCR检测
- Keywords:
- Helan Mountain East Region of Ningxia; wine grape virus diseases; natural incidence investigation in the field; grapevine leafroll-associated virus(GLRaVs); RT-PCR detection
- 文献标志码:
- A
- 摘要:
- 对宁夏贺兰山东麓8个酿酒葡萄种植地区8个葡萄品种的病毒病田间自然发病情况进行调查，采用RT-PCR方法，检测了40份样品中GLRaV-1~5这5种葡萄卷叶伴随病毒的感染率。结果表明：宁夏贺兰山东麓酿酒葡萄主要病毒病有葡萄卷叶病、扇叶病和栓皮病，其中葡萄卷叶病尤为严重；不同酿酒葡萄种植区和品种间葡萄卷叶病的感染存在着差异，老葡萄种植区中品种间葡萄卷叶病发病率明显高于新葡萄种植区的品种；“蛇龙珠”和“黑比诺”是感染葡萄卷叶病毒的主要品种，发病率高达85.1%和52.7%；利用5种葡萄卷叶伴随病毒引物进行RT-PCR检测，检出3种病毒类型，且GLRaV-3的检出率最高，为87.5%。
- Abstract:
- Eight wine grape planting areas in Helan Mountain East Region of Ningxia for grape virus diseases natural infection situation were investigated,by adopting RT-PCR method to test 40 samples of five kinds of grape leaf roll virus infection from GLRaV-1 to 5.The results showed that the major wine grape virus diseases in Helan Mountain East Region of Ningxia include grapevine leaf roll disease,grapevine fan leaf disease and grapevine corky bark,meanwhile grapevine leaf roll disease was particularly serious.There was difference between different wine grape planting areas as well as wine grape varieties of grape leaf roll disease infection.And the varieties of leaf roll disease incidence in the old grape wine region were obviously higher than the new wine region;‘Cabernet Gernischt’ and ‘Pinot Gris’ were the main grape varieties with grape leaf roll-associated virus with incidence rate as high as 85.1% and 52.7%.By RT-PCR detection for five kinds of grape leaf roll-associated virus primers,three types of viruses were diagnosed with GLRaV-3 acting out the highest detection rate at 87.5%.
参考文献/References:
[1]顾沛雯,洪波,马永明,等.宁夏玉泉营地区葡萄卷叶病田间自然发病调查及检测[J].宁夏农学院学报,2001,3(22):21-23.
[2]徐美隆,陈春伶,谢军,等.宁夏贺兰山东麓地区葡萄卷叶病发病与危害调查[J].北方园艺,2015(22):118-121.
[3]沙月霞,王国珍,樊仲庆.贺兰山东麓酿酒葡萄病毒病田间发生状况调查[J].中国果树,2010(4):60-63.
[4]范旭东,董雅凤,张尊平,等.葡萄病毒分子检测技术研究进展[J].园艺学报,2014,41(5):1009-1019.
[5]沙月霞,王国珍,樊仲庆.贺兰山东麓主栽葡萄品种病毒病鉴定及健康状况评价[J].西北农业学报,2010,19(12):188-193.
[6]王建辉,刘建军,陈克玲,等.葡萄卷叶伴随3型病毒和葡萄A病毒的多重检测及其系统进化分析[J].园艺学报,2011,38(12):2401-2410.
[7]裴光前,董雅凤,张尊平,等.我国葡萄主栽区卷叶病相关病毒种类的检测分析[J].果树学报,2011,28(3):463-468.
[8]任芳,董雅凤,张尊平,等.葡萄病毒研究最新进展[J].园艺学报,2014,41(9):1777-1792.
[9]张丽,石磊,甘晓燕,等.葡萄脱毒培养与病毒检测技术研究进展[J].北方园艺,2012(4):174-177.
[10]徐章逸,王国平,刘亚萍,等.葡萄卷叶伴随病毒-3外壳蛋白的原核表达及其特异性抗血清的制备[J].植物病理学报,2010,40(2):129-134.
[11]顾沛雯,张军翔.葡萄卷叶病的研究进展[J].宁夏农学院学报,2003,1(24):73-75.
[12]裴光前,董雅凤,张尊平,等.4种葡萄卷叶伴随病毒多重RT-PCR检测[J].植物病理学报,2010,40(1):21-26.
[13]何应琴,陈文龙,周常勇,等.果树病毒病传毒媒介及防控技术研究进展[J].天津农业科学,2012,18(6):95-99.
[14]TSAI C W,ROWHANI A,GOLINO D A,et al.Mealybug transmission of grapevine leafroll viruses：An analysis of virus-vector specificity[J].Phytopathology,2010,100(8):830-834.
[15]任芳,董雅凤,张尊平,等.葡萄病毒研究最新进展[J].园艺学报,2014,41(9):1777-1792.相似文献/References:
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备注/Memo
第一作者简介：吕苗苗（1991-），女，硕士研究生，研究方向为生物防治与菌物资源利用。E-mail：miaomlv@163.com.责任作者：顾沛雯（1969-），女，博士，教授，现主要从事植物病理学和生物防治及微生物资源利用的教学和科研等工作。E-mail：gupeiwen2013@126.com.基金项目：宁夏教育厅资助项目（NGY14011）；宁夏“十三五”科技重大专项资助项目（NX2016）。