DU Yinxiang,HU Zehua.Study on Extraction and Antibacterial Activity of Antibacterial Substance From Ficus tikoua Bur.[J].Northern Horticulture,2017,41(14):150-155.[doi:10.11937/bfyy.20164461]
地枇杷抑菌物质提取及抑菌活性研究
- Title:
- Study on Extraction and Antibacterial Activity of Antibacterial Substance From Ficus tikoua Bur.
- Keywords:
- Ficus tikoua Bur.; antibacterial substances; orthogonal test; reflux method; antibacterial activity
- 文献标志码:
- A
- 摘要:
- 以地枇杷为试材，在单因素试验基础上，采用正交实验方法优化地枇杷中抑菌物质的乙醇回流提取工艺，利用牛津杯法检测提取物对金黄色葡萄球菌、大肠埃希菌、宋内志菌、白假丝酵母菌的抑菌效果，探讨了热力、紫外线对其抑菌活性的影响。结果表明：乙醇回流法的最佳提取工艺为65%乙醇、料液比1∶10 g?mL-1，75 ℃水浴加热0.5 h。提取物对金黄色葡萄球菌抑菌效果最好，对白假丝酵母菌无抑菌作用。随着提取物浓度的增加，其抑菌作用随之增强，对热力、紫外线有一定的稳定性。
- Abstract:
- Taking Ficus tikoua Bur. as test material,based on the single factor experiment，the ethanol reflux extraction technology of antibacterial substance from Ficus tikoua Bur. was optimized by orthogonal experiment.Bacteriostatic effect of the extracts was detected by Oxford Cup method against Staphylococcus aureus，Escherichia coli，Shigella sonnei, antibacterial effect of Candida albicans,and the effect of heat and ultraviolet radiation on its antibacterial activity were also investigated.The results showed that the optimum extraction process of ethanol reflux were 65% ethanol,solid-liquid ratio 1∶10 g?mL-1,0.5 hours heating in 75 ℃ water bath.The antibacterial effect of the extracts on Staphylococcus aureus was the best,and Candida albicans had no antibacterial effect.With the increase of the concentration of the extract，its antibacterial effect was enhanced，and it had a certain stability to the heat and ultraviolet radiation.
参考文献/References:
[1]张秀实,吴征镒.中国植物志:被子植物门.双子叶植物纲.桑科[M].北京:科学出版社,1997. [2]方志先,廖朝林.湖北恩施药用植物志[M].武汉:湖北科学技术出版社,2006. [3]冯义龙,万涛,朱华明.优良的护坡植物：地瓜榕[J].南方农业,2011,5(12):10. [4]吴家荣,邱德文.中国常用中草药彩色图谱[M].贵阳:贵阳科技出版社,1993. [5]郭良君,谭兴起,郑巍,等.地瓜藤化学成分研究[J].中草药,2011,42(9):1709-1711. [6]杨世波,张润芝,江志勇,等.地板藤根的化学成分研究[J].中成药,2014,36(3):554-558. [7]徐蔚,王培,李尚真,等.地果根茎化学成分研究[J].天然产物研究与开发,2011（23）:270-272． [8]田民义,彭礼军,俸婷婷,等.地枇杷石油醚层化学成分研究[J].山地农业生物学报,2014,33(2):89-91. [9]杜银香.恩施地枇杷水提取物及其萃取成分的抑菌活性研究[J].湖北民族学院学报（医学版）,2016,33(2):37-39. [10]曾超珍,刘志祥.枸骨叶抑菌物质的提取及抑菌作用研究[J].北方园艺,2009(8):129-131. [11]周晶,冯淑华.中药提取分离新技术[M].北京:科学出版社,2010. [12]闫绍悦,林树乾,傅剑,等.五味子提取液抑菌活性研究[J].中国实验方剂学杂志,2014,20(10):142-146．
备注/Memo
第一作者简介：杜银香（1976-），女，本科，实验师，研究方向为中草药抗菌作用。E-mail:yinxiangdu@163.com.责任作者：胡泽华（1975-），男，博士，副教授，研究方向为中草药化学成分与药理。E-mail:hzh75418427@126.com.基金项目：国家自然科学基金资助项目(81560703)。