WANG Fei,FENG Lijuan,YANG Xuemei,et al.Advances in the Characteristics，Causes，Mechanisms and Controls of Vitrified Fruit Trees in vitro in China[J].Northern Horticulture,2016,40(23):195-199.[doi:10.11937/bfyy.201623045]
我国果树玻璃化试管苗特征、成因、机理及控制研究进展
- Title:
- Advances in the Characteristics，Causes，Mechanisms and Controls of Vitrified Fruit Trees in vitro in China
- Keywords:
- fruit trees; tube seedlings; vitrification; mechanisms; controls
- 文献标志码:
- A
- 摘要:
- 该研究统计了我国已报道的出现玻璃化试管苗的果树种类多达50种，并基于近年果树工作者的相关研究，总结并系统论述了玻璃化试管苗的生理生化特性、形态解剖学特征、DNA的MSAP分析、成因、发生机理及控制措施，对系统研究果树试管苗玻璃化现象、增加转基因植株的遗传稳定性、保存和恢复珍贵材料无性系、提高次生代谢物产量和消除毒性及工厂化育苗都具有十分重要的意义。
- Abstract:
- In this study，statistic suggested that vitrification phenomenon existed in more than 50 fruit tree species reported in China.Based on previous research about fruit trees，the study introduced physiobiochemical characteristics，morphological and anatomical characteristics，MSAP analysis of DNA，causes，mechanisms and controls of vitrification.It had significance to the research of vitrified fruit trees in vitro，improvement of the genetic stability of transgenic plants，restoration of precious material clones and industrialized breeding.Besides，it was also important to eliminate toxicity and increase output of the secondary metabolites．
参考文献/References:
[1]PHILLIPS D J,MATHEWS G J.Growth and development of carnation shoot tips in vitro[J].Bot Gaz,1964,125:7-12． [2]HACKET W P,ANDERSON J M.Asepic multiplication and maintain of differential carnation shoot tissue derived from shoot apices[J].Proc Amer Soc Hort Sci,1967，90:365-369． [3]DEBERGH P,HARBAOUI Y,LEMEUR R.Mass propagation of globe artichoke (Cynara scolymus):Evaluation of different hypothesis to overcome vitrification with special reference to water potential[J].Physiol Plant,1981,53:181-187． [4]陶铭.组织培养中畸形胚状体及超度含水态苗的研究[J].西北植物学报,2001,21(5)：1048-1058． [5]邱运亮,段鹏慧,赵华.植物组织快繁技术[M].北京：北京化学工业出版社，2010:58． [6]赵春梅.植物组织培养方法生产药用次生代谢产物研究进展[J].现代生物医学进展,2008(8)：790-795． [7]张真.葡萄细胞培养及其次生代谢产物白藜芦醇的代谢调控[D].兰州:甘肃农业大学，2008． [8]丰锋.试管苗玻璃化现象的研究进展[J].北方园艺，2003(3):71-73． [9]常有宏,张玉娇,李晓刚,等.‘黄冠’梨正常试管苗与玻璃化苗生理生化及超微结构的比较研究[J].园艺学报，2011，38(2)：225-232． [10]周书利,汤浩茹,代利娟,等.不同因素对草莓试管苗玻璃化的影响[J].北方园艺,2013(13):131-134． [11]葛红娟,梁美霞,戴洪义.玻璃化与正常苹果试管苗的叶片和茎的显微结构比较[J].植物生理学报,2010,46(3):223-227． [12]吕敏,夏秀英,徐品三,等.蓝莓玻璃化试管苗的显微结构及生理生化特性变化[J].植物生理学报,2014,50(4):453-460． [13]王宏伟.豆梨组织培养过程中玻璃化形成机理及其恢复技术研究[D].南京:南京农业大学,2011． [14]葛红娟.苹果玻璃化试管苗的特征特性、形成原因及DNA的MSAP分析[D].青岛:青岛农业大学,2011． [15]田田,刘鹏飞,乔光,等.玛瑙红樱桃离体快繁及组培苗的变异检测[J].西南大学学报(自然科学版),2015,37(10):30-37． [16]陈兵先,黄宝灵,吕成群,等.植物组织培养试管苗玻璃化现象研究进展[J].林业工程学报,2011,25(1):1-5． [17]李云,田砚亭,罗晓芳.珠美海棠试管苗玻璃化发生机理的初步研究[J].北京林业大学学报,1996,19(1):52-57． [18]余彭娜,周启贵,龙云,等.降低西瓜试管苗玻璃化因素的优化[J].西南大学学报(自然科学版),2009,31(10):35-38． [19]李佳莹,俞明亮,马瑞娟,等.桃组织培养中影响玻璃化产生的因素分析[J].江苏农业科学,2009(5):47-49． [20]朱新霞,孙黎,陶春萍.甜瓜离体再生继代培养中玻璃化现象的研究[J].西北植物学报，2006,26(7):1468-1472． [21]李好先,曹尚银,薛辉,等.‘绿波’核桃叶片愈伤组织的诱导研究[J].分子植物育种，2015,13(5):1060-1066． [22]王德芬,张梅,李鼎立,等.秋子梨叶片高效再生体系的构建[J].北方园艺,2016(4):97-101． [23]张春芬,潘天任,邓舒,等.草莓茎尖快速繁殖体系的研究[J].山西农业科学,2016,44(3):291-293,299． [24]孙清荣,孙洪雁,张力思,等.酸枣叶片不定梢形成及玻璃化的影响因素[J].西北植物学报,2010,30(5):1039-1044． [25]LEES R P,EVANS E H,NICHOLAS J R.Effects of the light environment on photosynthesis and growth in vitro[M].Dordrecht (The Netherlands):Kluwer Academic Publishers,1994:31-46． [26]李竹梅,王果萍.西瓜试管玻璃苗的成因及防止[J].山西农业科学,2008,32(3):43-46． [27]郑小华,廖明安,李明章,等.不同培养条件对软枣猕猴桃试管苗玻璃化的影响[J].资源开发与市场,2008,24(5):394-396． [28]林小苹,赖钟雄,黄浅.光质对植物离体培养的影响[J].亚热带农业研究,2008,4(1):73-80． [29]王菲,苑兆和,招雪晴,等.‘三白’石榴再生体系的构建[J].中国农学通报,2013,29(19):125-133． [30]周书利,汤浩茹,陈清,等.不同因素对草莓玻璃化试管苗恢复的影响[J].生物技术通报,2013(9):89-93． [31]刘霞,孙冲.木本植物试管苗玻璃化成因与控制研究进展[J].西南林业大学学报,2013，33(5):93-97． [32]丰锋,陈厝边.草莓离体培养中玻璃化的发生及克服措施研究[J].西南大学学报(自然科学版),2007,29(4):78-82． [33]郭玉朋.植物光呼吸途径研究进展[J].草业学报,2014,23(4):322-329． [34]LU G Y,WU X M,CHEN B Y.Detection of DNA methylation changes during seed germination in rapeseed (Brassiea napus)[J].Chinese Sci Bull,2006,51(2):182-190． [35]杨金兰,柳李旺,龚义勤,等.镉胁迫下萝卜基因组DNA甲基化敏感扩增多态性分析[J].植物生理与分子生物学学报,2007,33(3):219-226． [36]ZILBEMAN D,GEHRING M,TRAN R K.Geneme-wide analysis of Arabidopsis thaliana DNA methylation uneoyers a interdependence between methylation and transcription[J].Nat Genet,2007,39(1):61-69. [37]毕云,苏艳,张艺萍,等.蓝莓组织培养过程中玻璃化现象的防止技术研究[J].西南农业学报,2014,27(6):2539-2542． [38]曹善东.组培条件对草莓脱毒试管苗玻璃化影响的研究[J].山东农业大学学报(自然科学版),2006,37(2)：172-174． [39]任东植,李峰,曲运琴,等.影响枣组培苗玻璃化的几个因素及其防治[J].植物生理学报,2000,36(1):21-23． [40]石晓云.防止小果型西瓜组培苗玻璃化的研究[J].北方园艺，2011(9)：152-154． [41]齐红岩,贾卓男,陈岩.网纹甜瓜试管苗玻璃化成因研究[J].河南农业科学,2009(3):86-92． [42]孙阳,魏海蓉,程淑云,等.蓝莓组培苗玻璃化及恢复的研究[J].山东农业科学,2008(3):61-63． [43]林贵美,李小泉,邹瑜,等.番木瓜玻璃化组培苗逆向生长培养[J].中国南方果树,2009,38(2):23-24． [44]马永利.毛桃玻璃化试管苗恢复及叶柄再生研究[D].武汉:华中农业大学,2015． [45]段艳欣.苹果M7砧木组织培养与抗性筛选[J].江苏农业科学,2014,42(10):52-54．
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备注/Memo
第一作者简介：王菲（1983-)，女，硕士，助理研究员，现主要从事果树栽培生理与生物技术等研究工作。E-mail:yuanyi-fei@163.com．责任作者：尹燕雷（1976-)，男，硕士，副研究员，现主要从事果树与园林植物栽培与育种等研究工作。E-mail:yylei66@sina.com．基金项目：山东省自然科学基金资助项目（ZR2015YL056，ZR2014YL022)；山东省星火科技示范资助项目（2015XH002)；山东省果树研究所所长科研基金资助项目（2016KY04)。