SUN Zhaohui,MA Anfeng,CHENG Fei,et al.Rapid Detection of〖STHX〗 Crr3 Gene Against Clubroot Disease by PCR and Selection of Corresponding Germplasm in Chinese Cabbage (Brassica pekinensis L.)[J].Northern Horticulture,2016,40(22):120-123.[doi:10.11937/bfyy.201622030]
大白菜抗根肿病基因Crr3的PCR快速检测与种质资源筛选
- Title:
- Rapid Detection of〖STHX〗 Crr3 Gene Against Clubroot Disease by PCR and Selection of Corresponding Germplasm in Chinese Cabbage (Brassica pekinensis L.)
- Keywords:
- Chinese cabbage; clubroot disease; Crr3 gene; molecular markers; germplasm
- 文献标志码:
- A
- 摘要:
- 以抗根肿病大白菜自交系‘YM-7’和感病自交系“冠291”及其杂交F1、F2代和24份国内外引进的大白菜种质资源为试材，采用人工接种和分子标记方法，研究了2对与抗根肿病基因Crr3紧密连锁分子标记的稳定性及24份新材料中Crr3基因的情况。结果表明：引物OPC11-2S在‘YM-7’中扩增出1条1.3 kb片段，在“冠291”中扩增出1条1.0 kb片段，在其F1代中同时扩增出1.3 kb和1.0 kb的片段，该分子标记在F2代中的鉴定结果与人工接种鉴定结果一致，利用引物OPC11-2S能准确标记出大白菜种质资源中Crr3基因，而且能够区分出Crr3基因的纯合性和杂合性，共选出2份含有杂合Crr3基因的新种质资源。
- Abstract:
- The resistant line ‘YM-7’ and the susceptible line ‘Guan 291’，their F1，F2 generations and other 24 germplasms from foreign and domestic companies were used as test materials.Molecular marking method and artificial inoculation method were applied to select effective molecular marker to screen Crr3 gene from two published candidates.The results showed that 1.3 kb amplification fragment in ‘YM-7’,1.0 kb fragment in ‘Guan 291’ and both 1.3 kb and 1.0 kb fragments in their F1 generation were amplified with primer OPC11-2S.The results of artificial test and molecular marker screening for F2 generation of ‘YM-7×Guan 291’ were consistent.The primer OPC11-2S could not only identify the existence of Crr3 gene，but also could test the homozygosis or heterozygosis of Crr3 gene in germplasms accurately.Two germplasms with heterozygous Crr3 resistant gene were manifested.
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备注/Memo
责任作者：程斐（1969-)，男，博士，副教授，研究方向为十字花科蔬菜杂种优势利用。E-mail:chengfei246246@163.com.基金项目：山东省良种工程资助项目(鲁科字[2014]96号)；山东省现代农业产业技术体系资助项目（SDAIT-02-022-01)。