ZHANG Enliang,WANG Peng,LI Ya,et al.Development and Utility of EST-SSR Markers in Lagerstroemia indica[J].Northern Horticulture,2016,40(22):107-111.[doi:10.11937/bfyy.201622027]
紫薇EST-SSR标记的开发与利用
- Title:
- Development and Utility of EST-SSR Markers in Lagerstroemia indica
- 文献标志码:
- A
- 摘要:
- 以紫薇为试材，利用MISA软件对转录组测序获得45 308条unigenes中的SSR特征进行分析。结果表明:9 074条unigenes存在10 905个SSR位点，1 486条unigenes含有2个以上的SSR位点，550个位点是复合重复位点；从7 620个SSRs位点中设计出4 501对SSR引物；随机选择68对SSR引物进行检测，其中61对引物可以成功的扩增出清晰可用的条带，28对引物有多态性。
- Abstract:
- Lagerstroemia indica was used as test material,the EST-SSR molecular markers of Lagerstroemia indica were sought from transcriptome sequencing,45 308 unigenes were analyzed by MISA.The results showed that a total of 10 905 EST-SSRs were recognized from 9 074 unigenes,1 486 sequences contained more than one SSR and 550 SSRs presented in compound formation.In this study，a total of 4 501 primer pairs were designed from 7 620 SSRs.To validate SSR markers，68 primer pairs were randomly selected to PCR amplification and 61 SSR markers were amplified successfully.Of these validated SSR markers，28 primer pairs were detected polymorphism.
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备注/Memo
第一作者简介：张恩亮（1994-)，男，安徽六安人，硕士研究生，研究方向为园林植物。E-mail：everzel1994@163.com.责任作者：李亚（1969-)，男，安徽颍上人，博士，研究员，研究方向为园林植物。E-mail：yalicnbg@163.com.基金项目：科技部农业科技成果转化资金资助项目(2013GB2C100185)；江苏省农业科技自主创新资金资助项目(CX(14)2032)；江苏省林业三新工程资助项目(LYSX[2015]13)。