ZHANG Qianqian,YU Xiao,HE Xueqin.Influence of Hydrogen Peroxide Traps on Antioxidant System in Different Organs of Dianthus chinensis L.Under H2O2 Treatment[J].Northern Horticulture,2016,40(22):82-86.[doi:10.11937/bfyy.201622021]
过氧化氢清除剂对H2O2处理下中国石竹幼苗不同器官抗氧化系统的影响
- Title:
- Influence of Hydrogen Peroxide Traps on Antioxidant System in Different Organs of Dianthus chinensis L.Under H2O2 Treatment
- Keywords:
- Dianthus chinensis L.; hydrogen peroxide; dimethylthiourea; diphenyleneiodonium chloride; scavenger enzymes
- 文献标志码:
- A
- 摘要:
- 以中国石竹为试材，采用25 mmol?L-1 二甲基硫脲 (dimethylthiourea，DMTU) 和3 mmol?L-1 二苯基氯化碘盐 (diphenyleneiodonium chloride，DPI)对其幼苗预处理2 h后再置于5 mmol?L-1 H2O2中处理6 h，测定DMTU和DPI预处理对H2O2处理下中国石竹幼苗不同器官的H2O2和O?[TX-*4]2含量以及相关清除酶活性的方法，探究减少H2O2含量对氧化胁迫下石竹幼苗生理活性的影响，以期为石竹抗逆栽培提供理论依据。结果表明：25 mmol?L-1 DMTU和3 mmol?L-1 DPI预处理降低了正常条件下叶片和根的H2O2和O?[TX-*4]2含量，且在根中达显著差异水平（P<0.5)；5 mmol?L-1的外源H2O2处理显著增加了叶中H2O2含量和根中O?[TX-*4]2含量（P<0.5)，25 mmol?L-1 DMTU和3 mmol?L-1 DPI预处理则降低了5 mmol?L-1 H2O2处理下叶片和根部的H2O2和O?[TX-*4]2含量；5 mmol?L-1的外源H2O2处理显著增加了叶片SOD活性和根部SOD、APX活性（P<0.5)，25 mmol?L-1 DMTU和3 mmol?L-1 DPI预处理降低了5 mmol?L-1 H2O2处理下石竹叶中SOD、APX活性，且25 mmol?L-1 DMTU预处理显著降低了根部的APX活性(P<0.5)，表明降低H2O2和O?[TX-*4]2含量可降低相应清除酶的活性。因此，外施DMTU和DPI能不同程度缓解H2O2处理导致的内源H2O2和O?[TX-*4]2含量的积累，且25 mmol?L-1 DMTU清除H2O2的效果比3 mmol?L-1 DPI好。
- Abstract:
- Taking Dianthus chinensis L.as material，with 25 mmol?L-1 DMTU and 3 mmol?L-1 DPI for 2 hours were pretreated and 5 mmol?L-1 H2O2 for 6 hours were treated，then the contents of H2O2 and O?[TX-*4]2and related enzyme activity were measured，the influence of reducing H2O2 content on physiological activity under oxidative stress in Dianthus chinensis L.seedlings were studied，in order to provide theoretical basis for cultivation of Dianthus chinensis L.under adversity.The results showed that the contents of H2O2 and O?[TX-*4]2 in the leaves and roots were reduced by pretreatment of 25 mmol?L-1 DMTU and 3 mmol?L-1 DPI under normal conditions and levels of reduce were significant in the roots (P<0.5)；treatment with 5 mmol?L-1 H2O2 led to the significant increase of the content of H2O2 in leaves and the content of O?[TX-*4]2in roots (P<0.5)，but pretreatment with 25 mmol?L-1 DMTU and 3 mmol?L-1 DPI declined both in the two organs；the enzyme activities of SOD in leaves and SOD,APX in roots increased significantly under 5 mmol?L-1 H2O2 treatment (P<0.5)，but the enzyme activities of SOD and APX in leaves were declined by pretreatments of 25 mmol?L-1 DMTU and 3 mmol?L-1 DPI and the enzyme activity of APX in roots was significantly declined by pretreatments of 25 mmol?L-1 DMTU (P<0.5)，it suggested that the contents of H2O2 and O?[TX-*4]2 had positive relations with the activities of their scavenger enzymes.Therefore，DMTU and DPI could alleviate the accumulation of H2O2 and O?[TX-*4]2content induced by H2O2 treatment with different levels，and the former had the better effect of alleviation.
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备注/Memo
第一作者简介：张倩倩（1992-)，女，硕士研究生，研究方向为园艺植物逆境生理。E-mail：280393636@qq.com.责任作者：贺学勤（1970-)，女，博士，副教授，现主要从事园艺植物逆境生理等研究工作。E-mail：xueqinhe@imau.edu.cn.基金项目：国家自然科学基金资助项目（31260486)；中国农业大学-内蒙古农业大学合作基金资助项目（ZN201102)。