SU Rong,HAO Juan,QIN Lijin,et al.Allelochemicals Isolation of Acetone Extracts From Fresh Parsley Root and Allelopathic Effect on Fusarium oxysporum f.sp. cucumberinum[J].Northern Horticulture,2016,40(21):120-124.[doi:10.11937/bfyy.201621030]
西芹鲜根丙酮浸提液化感物质分离及其对黄瓜枯萎病菌的化感作用
- Title:
- Allelochemicals Isolation of Acetone Extracts From Fresh Parsley Root and Allelopathic Effect on Fusarium oxysporum f.sp. cucumberinum
- Keywords:
- fresh parsley root; acetone extracts; Fusarium oxysporum f.sp.cucumerinum; allelopathy; allelochemicals
- 文献标志码:
- A
- 摘要:
- 以西芹鲜根为试材，采用丙酮浸提法,分离了西芹鲜根丙酮浸提液中的化感物质，并研究了化感物质对黄瓜枯萎病菌的抑制作用。结果表明：第5次层析抑制效果最佳的4个流分分别是RA32462、RA33444、RA98898、RA910664，其抑制率与丙酮对照相比分别提高了33.92%、35.80%、31.11%、32.15%。第6次层析抑制效果最佳的4个流分分别是RA324625、RA334448、RA988981、RA9106643，其抑制率与丙酮对照相比分别提高了35.10%、26.48%、34.06%、28.76%。通过对第6次层析的4个最佳流分的丙酮浸提物进行GC-MS检测，共鉴定出酸类、酯类、酚类和烷烃类共4类14种具有化感抑制作用的物质。
- Abstract:
- In this experiment,acetone extracts from fresh parsley roots were examined after the 5th and 6th cycles purification in column chromatography.The various column fractions were detected with F.oxysporum f.sp.cucumberinum.In terms of allelopathic inhibition effect and identified allelochemicals the experiment screened the best fractions of the 6th chromatography using GC-MS.Four best fractions were selected by the 5th extracts.They were RA32462,RA33444,RA98898,RA910664.The allelopathy inhibition effects compared with CK increased 33.92%,35.80%,31.11% and 32.15% respectively.And four best fractions were selected by the 6th extracts,they were RA324625,RA334448,RA988981,RA9106643.The allelopathic inhibition effects compared with CK increased 35.10%,26.48%,34.06%,28.76% respectively.There were 14 allelochemicals in four kinds which included acids，esters，phenols and alkanes were identified by GC - MS detection.
参考文献/References:
[1]闫霜,吴洪生,周晓冬,等.黄瓜枯萎病生物防治研究进展[J].山东农业科学,2011(1):86-92. [2]钱程,云兴福,高晓敏,等.西芹鲜根浸提液作用后黄瓜枯萎病菌弱毒菌株的筛选[J].中国生态农业学报,2014,21(5):606-614. [3]王永,陈磊,云兴福,等.西芹根物质水浸提液二次层析物对黄瓜枯萎病菌的化感作用[J].内蒙古农业科技,2013(2):33-35. [4]吕春霞,杨文叔,慕小倩.植物化感作用及其在杂草防治中的应用[J].陕西农业科学,2002(12):18-20. [5]郝静,高晓敏,马立国,等.西芹根物质四次醇层物对黄瓜枯萎病菌的化感作用[J].内蒙古农业大学学报(自然科学版),2015,36(3):26-32. [6]高晓敏,王琚钢,李杰,等.西芹鲜根丙酮浸提物层析流分对黄瓜枯萎病菌的化感作用以及化感物质鉴定[J].中国生态农业学报,2014,22(11):1364-1371. [7]白淑兰,阎伟,胡永健.菌根研究及内蒙古大青山外生菌根资源[M].呼和浩特:内蒙古人民出版社,2011. [8]李蕾,云兴福,陈磊.西芹根物质丙酮浸提液二次层析物对黄瓜枯萎病菌的化感作用[J].内蒙古大学学报(自然科学版),2012,43(6):639-644. [9]高晓敏,王琚钢,马立国,等.尖孢镰刀菌致病机理和化感作用研究进展[J].微生物学通报,2014,41(10):2143-2148. [10]魏彦梅.西芹鲜根及根际区物浸提液对黄瓜枯萎病菌化感作用机理的研究-浸提液处理后黄瓜叶片内防御酶活性的变化[D].呼和浩特:内蒙古农业大学,2012. [11]谢星光,陈晏,卜元卿,等.酚酸类物质的化感作用研究进展[J].生态学报,2014,53(77):6417-6428. [12]YE S F,YU J Q,PENG Y H，et al.Incidence of Fusarium wilt in Cucumis sativus L.,is promoted by cinnamic acid,an autotoxin in root exudates[J].Plant and Soil,2004,263(1):143-150. [13]刘艳霞,李想,蔡刘体,等.烟草根系分泌物酚酸类物质的鉴定及其对根际微生物的影响[J].植物营养与肥料学报,2016,22(2):418-428. [14]周宝利,张健,刘娜,等.己二酸二异丁酯对辣椒种子萌发及枯萎菌的化感效应[J].生态学杂志,2009,28(11):2273-2278. [15]董晓宁,高承芳,张晓佩,等.多花黑麦草根系抑草潜力评价及其化感物质分析[J].草业学报,2013,22(4):61-68. [16]饶洪冲,段琰,朱征宇.苯酚消毒剂中邻苯基苯酚和对叔戊基苯酚的高效液相色谱测定法[J].医药病理学报,2013,8(2):46-47. [17]HEINDEL J J，POWELL C J.Phthalate ester effects on rat Sertoli cell function in vitro：Effects of phthalate side chain and age of animal[J].Toxicology and Applied Pharmacology，1992,115:116-123. [18]周宝利,韩琳,尹玉玲,等.化感物质棕榈酸对茄子根际土壤微生物组成及微生物量的影响[J].沈阳农业大学学报,2010,41(3):275-278.
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备注/Memo
第一作者简介：苏荣（1992-)，女，硕士研究生，研究方向为高寒地区蔬菜栽培与生理。E-mail:1570371884@qq.com.责任作者：云兴福（1958-)，男，回族，教授，博士生导师，现主要从事高寒地区蔬菜栽培与生理等研究工作。E-mail:yxf5807@163.com.基金项目：国家农业科技成果转化资金资助项目（2014GB2A400716）；内蒙古自治区应用技术研究与开发基金资助项目（20150136）。