XIAO Xiou,LIN Wenqiu,LI Wei,et al.Construction and Evaluation of Yeast Two Hybrid cDNA Library for Eggplant Root Inoculation With R.solanacearum[J].Northern Horticulture,2016,40(21):102-105.[doi:10.11937/bfyy.201621026]
感染青枯病病原菌R.solanacearum的茄子酵母双杂交文库构建及评价
- Title:
- Construction and Evaluation of Yeast Two Hybrid cDNA Library for Eggplant Root Inoculation With R.solanacearum
- 关键词:
- 茄子; R.solanacearum; 酵母双杂交; cDNA文库
- Keywords:
- eggplant; R.solanacearum; yeast two hybrids; cDNA library
- 文献标志码:
- A
- 摘要:
- 以高抗青枯病的茄子高代自交系‘Q31-1’为试材，采用同源重组法构建了感染青枯病原菌R.solanacearum的茄子根部酵母双杂交cDNA文库，以期为研究茄子（Solanum melongena L.）与青枯病病原菌R.solanacearum的互作机制奠定基础。结果表明：文库库容大于1.25×107 CFU，平均插入片段大于1.5 kb，随机挑选24个单克隆进行PCR检测，阳性率为100%。表明该文库可用于进行下一步互作蛋白筛选的研究。
- Abstract:
- Taking ‘Q31-1’ eggplant as test material，the yeast two hybrid cDNA library was constructed after the eggplant inoculation with R.solanacearum,in order to research the interaction mechanism between the eggplant and R.solanacearum.The results showed that the library contained 1.25×107 CFU with 100% recombination rate based on 24 randomly selected clones.The result of PCR showed that length of insert cDNA fragments was greater than 1.5 kb.The results demonstrated that the library could be used to screen the interaction protein.
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备注/Memo
第一作者简介：肖熙鸥(1988-)，男，硕士，研究实习员，研究方向为蔬菜遗传育种及生物技术。E-mail:xiao-forlearning@163.com.基金项目：中央级公益性科研院所基本科研业务费专项资金（中国热带农业科学院南亚热带作物研究所）资助项目（1630062015005）；海南省自然科学基金资助项目（20153120）；国家自然科学基金资助项目（31501774）。