CHENG Lijun,LI Shihui,HU Zhifang,et al.Optimization of Cultivation Condition in Gastrodia elata Seed-germinating Strain[J].Northern Horticulture,2016,40(19):175-177.[doi:10.11937/bfyy.201619044]
一株乌天麻共生萌发菌培养条件的优化
- Title:
- Optimization of Cultivation Condition in Gastrodia elata Seed-germinating Strain
- 文献标志码:
- A
- 摘要:
- 以天麻共生萌发菌MY-001为试材，采用单因素试验和正交实验分析法，以菌丝生长速率和菌丝体生物量为主要指标，对萌发菌MY-001培养环境条件及培养基营养条件进行优化。结果表明：MY-001最适宜的环境条件为温度25 ℃，培养基质含水量200%下进行暗培养；MY-001最易利用的氮源和碳源分别是酵母膏、葡萄糖，复合维生素B也能促进菌丝生长，最佳的营养条件为酵母膏3 g?L-1、葡萄糖20 g?L-1、复合维生素B 0.005 g?L-1，通过多元回归分析建立回归方程（Y=0.362+0.084A-0.014B-0.007C），方差分析显示方程为极显著。
- Abstract:
- Gastrodia elata seed-germinating strain MY-001 was used as test material，the cultivation conditions of MY-001 was studied according to the index of mycelial growth rate and mycelial biomass by single factor experiment and orthogonal experiment.The results showed that the optimum growth environmental conditions were 25 ℃，200% water content，under dark cultivation;glucose and yeast extracts were the optional carbon source and nitrogen sources.The optimum growth nutrient conditions were determined yeast extracts 3 g?L-1，glucose 20 g?L-1，composite vitamin B 0.005 g?L-1.Through multiple regression analysis to establish regression equation (Y=0.362+0.084A-0.014B-0.007C)，variance analysis showed significant equation was very significant.
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备注/Memo
第一作者简介：程立君（1986-），男，湖南岳阳人，硕士，助教，研究方向为食用菌与中草药资源开发。E-mail:chenglijun224@163.com。