LI Zhiliang,ZHENG Zhuang,YANG Guliang,et al.Cloning and Analysis of α-momorcharin Gene Promoter From Momordica chanrantia L.[J].Northern Horticulture,2016,40(18):99-102.[doi:10.11937/bfyy.201618024]
苦瓜a-苦瓜素基因启动子的克隆和序列分析
- Title:
- Cloning and Analysis of α-momorcharin Gene Promoter From Momordica chanrantia L.
- Keywords:
- Momordica charantia L.; α-momorcharin; chromosome walking; promoter
- 文献标志码:
- A
- 摘要:
- 以苦瓜嫩叶为试材，采用染色体步移法，克隆了α-苦瓜素基因启动子序列，并对其进行序列分析。结果表明：该启动子长12 kb，含有2个TATA box，2个CAAT box，以及参与茉莉酸甲酯代谢、防御和压力反应、热胁迫反应、光反应调节、昼夜节律调节等的顺式作用元件。
- Abstract:
- Taking tender leaf of M.charantia as material,a total of 12 kb alpha-momorcharin gene promoter was cloned by chromosome walking.The results showed that two TATA boxes，two CAAT boxes，and elements involving in jasmine acid methyl ester metabolism，defense，stress response，heat stress response，light response regulator，circadian rhythm regulation，had been identified.
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备注/Memo
第一作者简介：李志良(1983-)，男，本科，助教，现主要从事植物分子生物学等研究工作。E-mail:lizhiliang04@163.com.责任作者：王书珍(1984-)，女，博士，讲师，现主要从事植物遗传学等研究工作。E-mail：wangshuzhen710@whu.edu.cn.基金项目：黄冈师范学院科学研究资助项目（2014022203）；黄冈师范学院发展规划处资助项目(xfg2015C22)；大别山特色资源开发湖北省协同创新中心团队资助项目（2015TD07）。