LIANG Weiling,LU Yanqi,CHEN Cuiguo,et al.Key Proliferation Technology of Prunus humilis Fine Strains Plantlets in vitro[J].Northern Horticulture,2016,40(17):103-106.[doi:10.11937/bfyy.201617024]
欧李优系试管苗增殖关键技术研究
- Title:
- Key Proliferation Technology of Prunus humilis Fine Strains Plantlets in vitro
- Keywords:
- Prunus humilis; plantlets in vitro; proliferation; key technology
- 文献标志码:
- A
- 摘要:
- 以欧李优系试管苗为试材，采用单因素试验设计，研究了不同生长调节物质组合、基本培养基类型、附加物和蔗糖浓度对试管苗生长状态和增殖效率的影响。结果表明：在MS＋6-BA 0.5 mg?L-1+KT 0.5 mg?L-1＋IBA 0.05 mg?L-1培养基上，附加水解乳蛋白（LH）300 mg?L-1、蔗糖35 g?L-1、琼脂5.8 g?L-1，为欧李优系试管苗增殖的最佳培养条件。
- Abstract:
- By single factor text，the effects of various basic culture medium，growth regulator combination，additions and sucrose concentration were studied on growth status and proliferation rate of Prunus humilis fine strains in vitro plants.The results indicated that the optimal medium of proliferation was MS＋6-BA 0.5 mg?L-1+KT 0.5 mg?L-1＋IBA 0.05 mg?L-1，additional LH 300 mg?L-1，sugar 35 g?L-1，agar 5.8 g?L-1.
参考文献/References:
[1]王有信.欧李综合开发前景与模式[J].山西果树，2010,31(1)：41-43. [2]张东为,贾天会,舒乔生.水保优良树种欧李的研究进展及今后研究方向[J].中国水土保持，2012，34（1）：45-47. [3]刘明,穆晓鹏,薛晓芳,等.欧李生物技术研究进展及其应用展望[J].落叶果树，2010,42(5):15-17. [4]庄丽娟,苏福才.欧李的组织培养与快速繁殖技术[J].内蒙古农业大学学报，2005，26(1)：16-19. [5]孙新政,申顺先,李庆伟,等.钙果4号欧李组织培养技术研究[J].果树学报,2007,24(1):80-83. [6]陈书明,姜英淑,王秋玉.欧李组培快速繁殖体系的建立[J].林业科技，2008，33(2)：59-62. [7]焦淑华,林丽华,李宝江.欧李绿枝组织培养与快速繁殖研究[J].北方园艺,2006(3):117-119. [8]刘霞,孙冲.木本植物试管苗玻璃化成因与控制研究进展[J].西南林业大学学报，2013,33(5):93-97.
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备注/Memo
第一作者简介：梁伟玲（1970-），女，河北邯郸人，实验师，现主要从事园艺植物组织培养等研究工作。E-mail:lwlsrx@163.com.基金项目：邯郸市科技局资助项目（1327101071-2）。