FANG Yuan,ZHANG Yahong,WANG Lihui,et al.Effect of Low Temperature Stress on Physiological and Biochemical Characteristics of Three Varieties of Cucumber Seedlings[J].Northern Horticulture,2016,40(17):30-36.[doi:10.11937/bfyy.201617007]
低温胁迫对三个黄瓜品种幼苗生理生化特性的影响
- Title:
- Effect of Low Temperature Stress on Physiological and Biochemical Characteristics of Three Varieties of Cucumber Seedlings
- Keywords:
- cucumber; variety; hypothermia; physiological index; effect
- 文献标志码:
- A
- 摘要:
- 针对宁夏地区日光温室冬春茬蔬菜栽培中经常出现低温导致产量品质下降问题，以温室常用黄瓜品种“德尔99”“德尔99”嫁接苗、“德尔100”幼苗为试材，根据宁夏冬季温室温度变化，设定处理温度为23 ℃/6 ℃（昼温/夜温）和23 ℃/17 ℃恢复（同时为对照）进行智能人工气候箱模拟，低温胁迫分别持续2、4、6、8、10 d后恢复2、4、6 d，测定不同低温和恢复期黄瓜幼苗中丙二醛含量、相对电导率、脯氨酸含量和超氧化物歧化酶（SOD）活性，旨在探究黄瓜幼苗在低温条件下的适应性和耐受期，为筛选适宜当地日光温室冬春季种植的黄瓜品种提供依据。结果表明：3个〖JP4〗黄瓜品种的幼苗在低温胁迫下均出现不同程度的伤害，“德尔99”和“德尔100”黄瓜幼苗能耐受6 ℃低温胁迫2～4 d后恢复状态，“德尔99”嫁接苗低温胁迫8～10 d后恢复正常生长；采用隶属函数综合评价3个黄瓜品种幼苗抗寒性强弱为“德尔99”嫁接苗＞“德尔100”＞“德尔99”。
- Abstract:
- To discuss the problem of decline in vegetable yield and quality caused by the low temperature in greenhouse cultivation in Ningxia.With common cucumber ‘Del 99’‘Del 99’ graft seedlings，and ‘Del 100’ seedlings as materials.According to Ningxia winter greenhouse temperature，processing temperature set as 23 ℃/6 ℃ (day temperature/night temperature) and 23 ℃/17 ℃ recovery (simultaneously control) for intelligent artificial climate chamber simulation.Low temperature stress were sustained for 2，4，6，8，10 days then recovered for 2，4，6 days.The measurement contained the MDA content，relative conductivity，proline content and superoxide dismutase (SOD) activity in different temperatures and convalescent cucumber seedlings.The results showed that three different species of cucumber showed various damage under low temperature stress，‘Del 99’ and ‘Del 100’ cucumber seedlings could tolerate 6 ℃ of low temperature stress for 2-4 days then restored the state，‘Del 99’ graft seedlings could tolerate low temperature stress for 8-10 days to restore normal growth；use membership function comprehensive evaluation to evaluate seedling cold resistance in 3 species of cucumber seedlings：grafted ‘Del 99’>‘Del 100’>‘Del 99’.It aimed to explore the adaptability and tolerance of cucumber seedlings under low temperature conditions so that it could provide the basis for screening the suitable solar greenhouse cucumber species grown in local in winter and spring.
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备注/Memo
第一作者简介：方媛（1990-），女，安徽桐城人，硕士研究生，研究方向为蔬菜学。E-mail：41359185@qq.com.责任作者：张亚红（1965-），女，宁夏平罗人，博士，教授，研究方向为设施园艺环境。E-mail：zhyhcau@sina.com.基金项目：国家科技支撑计划资助项目（2014BAD05B02）。