WANG Meng,LIANG Xue.Cloning and Bioinformatics Analysis of the GBSS Gene From Chinese Chestnut[J].Northern Horticulture,2016,40(16):99-103.[doi:10.11937/bfyy.201616026]
板栗颗粒型淀粉结合酶基因cDNA的克隆与生物信息学分析
- Title:
- Cloning and Bioinformatics Analysis of the GBSS Gene From Chinese Chestnut
- Keywords:
- Castanea mollssina; granule-bound starch synthase; clone; sequence analysis
- 文献标志码:
- A
- 摘要:
- 以板栗为试材，采用RT-PCR技术，克隆了颗粒性淀粉结合酶（GBSS）的基因，并进行了生物信息学分析。结果表明：从板栗果实中分离了GBSS基因cDNA全长，命名为Cgbss，登录GenBank号为KU162945。该基因全长2 085 bp，编码区全长为1 836 bp，编码611个氨基酸。生物信息学分析表明该基因编码的蛋白质等电点和分子量为8.36和67.580 9 kDa，含有典型的GT1家族的糖基转移酶蛋白功能域。序列分析表明，该基因与所报道的植物GBSS I的基因有85%的同源性。
- Abstract:
- Taking chestnut as test material，GBSS gene was cloned from the first strand of Castanea mollssina seed’s cDNA through RT-PCR methods，and the bioinformatics characters were analysed.The results showed that a full length cDNA of Cgbss(GenBank accession number:KU162945) was obtained.The length of Cgbss gene was 2 085 bp，which contained an open reading frame encoding 611 amino acids.The bioinformatics analysis indicated that the pI and MW of animo acids encoded by Cgbss were predicted to be 8.36 and 67.580 9 kDa，respectively，and the protein had one GT1_glycogen_synthase domain.And the fragment had 85% homology identical to the already reported sequence of GBSS I.
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备注/Memo
第一作者简介：王猛（1980-），男，山东枣庄人，博士，讲师，现主要从事经济林和植物分子生物学等研究工作。E-mail：mengwang80@sina.com.基金项目：西南林业大学林学学位授权学科经费资助项目；云南省教育厅科学研究基金资助项目(2013y125);西南林业大学科研启动基金资助项目。