XIAO Long,CONG Peihua,ZHANG Caixia,et al.Protein Extraction of Apple Fruit Skin and Two-dimensional Electrophoresis Optimization[J].Northern Horticulture,2016,40(15):130-134.[doi:10.11937/bfyy.2016150032]
苹果果实表皮总蛋白提取及双向电泳方法的优化
- Title:
- Protein Extraction of Apple Fruit Skin and Two-dimensional Electrophoresis Optimization
- Keywords:
- apple; fruit skin; proteomics; two-dimensional electrophoresis (2-DE)
- 文献标志码:
- A
- 摘要:
- 以苹果果实表皮为试材，采用3种方法提取果实表皮总蛋白，并对双向电泳（2-DE）操作体系进行优化。结果表明：改良酚提法适于苹果果实表皮总蛋白的提取，优化后的2-DE分离体系为预制IPG胶条18 cm pH 4~7，上样量600 μg，改进后的等电聚焦程序，浓度16%的分离胶，适于苹果果实表皮总蛋白的2-DE分离。
- Abstract:
- Taking apple fruit skin as test material，and the suitable extraction of apple fruit skin protein was selected，and the two-dimensional electrophoresis (2-DE) operating steps of the protein sample were improved.The results showed that the modified phenol extraction was suited for the extraction of apple fruit skin protein，and the improved 2-DE operating system (IPG strip 18 cm pH 4-7，600 μg sample loading)，the improved program of isoelectric focusing，16% SDS-PAGE gel was suited for the separation of the protein.
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备注/Memo
第一作者简介：肖龙（1984-），男，博士研究生，研究方向为果树遗传育种。E-mail:1984xl@163.com.责任作者：丛佩华（1963-），男，博士，研究员，现主要从事果树遗传育种等研究工作。E-mail：congph@163.com.基金项目：农业部现代农业产业技术体系建设专项资助项目(CARS-28)；国家科技支撑计划资助项目(2013BAD02B01)。