SUN Jingbo,ZHANG Qiuju,ZHANG Lianxue.Effect of Rhizosphere Soil Chemical Properties on Contents of Polysaccharides in the Root of Saposhnikovia divaricata[J].Northern Horticulture,2016,40(13):162-165.[doi:10.11937/bfyy.201613042]
根际土壤化学性质对防风多糖含量的影响
- Title:
- Effect of Rhizosphere Soil Chemical Properties on Contents of Polysaccharides in the Root of Saposhnikovia divaricata
- 文献标志码:
- A
- 摘要:
- 以防风为试材,采用水提醇沉法提取防风多糖,苯酚-硫酸-紫外分光光度法测定多糖含量;根据国家标准方法测定防风根际土壤pH,速效N、P、K,全N及有机质的含量,采用SPSS 13.0软件分析不同时间、不同采集地的防风根际土壤化学性质对防风多糖含量的影响。结果表明:防风多糖含量随采集地及采集时间不同而差异显著。只有10月防风多糖含量与其根际土壤速效K含量之间呈显著非线性相关(P<0.01)。土壤pH、水解N与速效P之间,水解N、速效P与速效K之间,水解N、速效P与有机质之间呈极显著相关(P<0.01);速效K与有机质之间的相关性随时间的变化而变化,其中8月时P<0.01,10月时P<0.05;水解N、有机质与全N之间呈显著相关(P<0.05)。防风多糖含量在不同采收期受其根际土壤的影响不同,采集于10月中旬的防风多糖含量受其根际土壤速效K影响显著。土壤中各养分含量适宜且稳定时,可提高多糖产率。
- Abstract:
- Taking Saposhnikovia divaricata as test material,water extraction and alcohol precipitation method was used to extract polysaccharides in Saposhnikovia divaricata (S.divaricata) and the content of polysaccharides was determined by phenol sulfuric acid ultraviolet spectrophotometry.According to standard of China,the pH,rapidly available nitrogen(N),phosphorus(P)and kalium(K),total nitrogen and organic matter contents in rhizosphere soils of S.divaricata were measured.The effects of rhizosphere soil chemical properties on the contents of polysaccharides in S.divaricata collected from different regions in different harvest times were analyzed with SPSS 13.0 software.The results showed that the contents of polysaccharides in the roots of S.divaricata were significantly different with different collection regions and times.Only the polysaccharides contents of S.divaricata collected in October showed a significantly non-linear correlation with the rapidly available K content in the rhizosphere soils(P<0.05).The pH and hydrolysable nitrogen content with the content of available phosphorus,the hydrolysable nitrogen and available phosphorus contents with the available kalium content,and the hydrolysable nitrogen and available phosphorus contents with the organic matter content were significantly correlated(P<0.01).The correlation of available kalium with the organic matter changed with the change of the collecting time,in which the differences in August(P<0.01)and October(P<0.05)were significant.Significant correlations of the hydrolysable nitrogen and organic matter contents with the total nitrogen content were found(P<0.05).The polysaccharides contents of S.divaricata collected at different harvest times could be affected by the different rhizosphere soils,especially those collected in October were significantly influenced by the available potassium content of soils.The content optimization and stability of nutrients in the soil could improve the productivity of polysaccharides in the S.divaricata.
参考文献/References:
[1]国家药典委员会.中国药典[S].一部.北京:中国医药科技出版社,2010. [2]刘焕蓉,秦雪梅,王利平,等.玉屏风散配伍剂量变化与有效成分的相关性研究[J].中草药,2009,40(12):1922-1924. [3]李丽,桂语歌,时东方,等.防风中色原酮类化合物的抗氧化活性研究[J].时针国医国药,2010,21(9):2135-2137. [4]黎建斌,刘丽萍,丘振文.生防风挥发油抗炎止血作用的药理研究[J].新中医,2007,39(8):105-106. [5]葛卫红,郭建友,沈映君,等.荆防挥发油对炎症因子表达和调节的影响[J].中国药学杂志,2007,32(17):1777-1779. [6]张泽庆,田应娲,张静.防风多糖的抗氧化活性研究[J].中药材,2008,31(2):268-272. [7]李莉,周勇,张丽,等.防风多糖增强巨噬细胞抗肿瘤作用的实验研究[J].北京中医药大学学报,1999,22(3):38-40. [8]国家中医药管理局《中华本草》编委会.中华本草[M].上海:上海科学技术出版社,1999. [9]刘华,罗强,田嘉铭.不同产地防风多糖含量比较[J].时珍国医国药,2008,19(10):2464-2466. [10]国家林业局.中华人民共和国林业行业标准[S].北京:中国标准出版社,1999. [11]国家环境保护总局.中华人民共和国土壤环境质量标准[S].北京:中国标准出版社,1995. [12]HE L Z,YANG X E,STOFFELLA P J.Trace elements inagroecosystems and impacts on the environment[J].Journal of Trace Elements in Medicine and Biology,2005,19:125-140.
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备注/Memo
第一作者简介:孙晶波(1978-),女,博士,副教授,研究方向为药用植物药活性成分及质量评价。E-mail:sjb781219@163.com.基金项目:国家科技支撑资助项目(2011BAI03B01)。