[1]曹小燕,杨海涛.槐花多糖提取工艺优化及自由基清除活性研究[J].北方园艺,2016,40(13):141-146.[doi:10.11937/bfyy.201613037]
　CAO Xiaoyan,YANG Haitao.Study on Enzymatic-ultrasonic Assisted Extraction of Polysaccharide From Sophora japonica Linn. and Its Free Radical Scavenging Activity[J].Northern Horticulture,2016,40(13):141-146.[doi:10.11937/bfyy.201613037]
点击复制

槐花多糖提取工艺优化及自由基清除活性研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第40卷 期数: 2016年13 页码: 141-146 栏目: 出版日期: 2016-07-15
Title:
Study on Enzymatic-ultrasonic Assisted Extraction of Polysaccharide From Sophora japonica Linn. and Its Free Radical Scavenging Activity
作者:
曹小燕; 杨海涛
（陕西理工学院 化学与环境科学学院，陕西 汉中 723001）
Author(s):
CAO Xiaoyan; YANG Haitao
(School of Chemical and Environmental Science,Shaanxi University of Technology,Hanzhong,Shaanxi 723001)
关键词:
槐花多糖; 正交实验; 纤维素酶; 柠檬酸盐酸性缓冲溶液
Keywords:
Sophora japonica Linn. polysaccharide; orthogonal experiment; cellulase; citrate buffer solution
DOI:
10.11937/bfyy.201613037
文献标志码:
A
摘要:
以秦巴山区野生槐花为试材，以pH 6.0柠檬酸盐酸性缓冲溶液作为浸提溶剂，采用单因素试验结合正交实验方法研究了纤维素酶-超声辅助提取槐花多糖的工艺条件，并考察其对羟基自由基、超氧阴离子自由基和ABTS自由基正离子的清除活性及还原能力。结果表明：槐花多糖的最佳提取工艺为提取温度60 ℃、提取时间20 min、料液比1∶25 kg?L-1、纤维素酶10 mg?g-1，该条件下槐花多糖的提取率为17.1%，槐花多糖对ABTS自由基正离子、羟基自由基和超氧阴离子自由基的清除IC50值为0.047、0.75、0.87 g?L-1，优于同浓度条件下天然抗氧化剂维生素C。
Abstract:
Taking Sophora japonica Linn. from Qinba area as raw material,citrate buffer solution at pH 6.0 as the extraction solvent,based on single factor experiments and orthogonal test,the extraction and scavenging free radicals activities of polysaccharides from Sophora japonica Linn. by cellulase-ultrasonic were studied.The results showed that the optimum extraction technology of Sophora japonica Linn. polysaccharides were as follows,extraction temperature was 60 ℃,ultrasonic extraction time of 20 minutes,solid-liquid ratio of 1∶25 kg?L-1,cellulase dosage was 10 mg?g-1,under this condition,the extraction yield of polysaccharide was 17.1%.Scavenging free radicals activities results showed that the IC50 of ABTS+,?OH,O?[TX-*4]2 were 0.047 g?L-1,0.75 g?L-1 and 0.87 g?L-1 respectively,which was superior to the conventional antioxidants of ascorbic acid.The study proved that polysaccharides from Sophora japonica Linn. had good reducing capacity and scavenging radical activity. Keywords:Sophora japonica Linn. polysaccharide；orthogonal experiment；cellulase；citrate buffer solution

参考文献/References:

 

[1]贾佼佼,苗明三.槐花的化学、药理及临床应用[J].中医学报,2013,29(5):716-718.

[2]王秀丽,李桂风.槐花的营养与药用价值[J].东方食疗与保健,2014,10(5):4-5.

[3]马利华,贺菊萍,秦卫东,等.槐花提取物抗氧化性能研究[J].食品科学,2007,28(9):75-77.

[4]胡喜兰,姜琴,尹福军,等.正交实验优选槐花多糖的最佳提取工艺及抑菌活性研究[J].食品科技,2012,37(4):164-167.

[5]李桂华,李丽萍,崔建军.原子吸收法测定槐花和香椿芽中微量元素含量[J].仪器仪表与分析监测,2005(4):37-38.

[6]宋永芳,罗嘉梁,解荷锋.刺槐花的活血成分研究[J].林产化学与工业,1992(4):320-326.

[7]赵国华,陈宗道,李志孝,等.活性多糖的研究进展[J].食品与发酵工业,2001,27(7):45-48.

[8]李环宇,王敏,李五霞,等.超声波辅助酸性缓冲液浸提木枣多糖的工艺优化[J].食品与机械,2015,31(3):179-184.

[9]王丽华,段玉峰,马艳丽.槐花多糖的提取工艺及抗氧化活性研究[J].西北农林科技大学学报(自然科学版),2012,36(8):213-217.

[10]王红庆,赵丽平,朱晓明.槐花多糖提取工艺的研究[J].湖北农业科学,2011,50(13):2733-2735.

[11]RE R,PELLEGRINI N,PROTEGGENTE A,et al.Antioxidant activity applying an improved ABTS radical cation decolorization assay[J].Free Radical Biology and Medicine,1999,26(9/10):1231-1237.

[12]SMIRONFF N,CUMBES Q J.Hyroxyl radical scavenging activity of compatiblesolutes[J].Phytochemistry,2011,28(21):1057-1060.

[13]BUETLER T M,KRAUSKOPF A,RUEGG U T.Role of superoxide as a signaling molecule[J].Physiology,2004,19(3):120-123.

[14]OYAIZU M.Studies on products of browning reaction-antioxidative activities of products of browning reaction prepared from glucosamine[J].Japanese Journal of Nutrition,1986,40(44):307-315.

[15]徐建国,田呈瑞,胡青平,等.响应面法优化槐花水溶性多糖的超声波辅助提取工艺[J].食品科学,2011,32(4):112-116.

[16]王晓林,钟方丽,薛健飞,等.酶法提取刺玫果总黄酮工艺研究[J].北方园艺,2015(4):136-139.

相似文献/References:

[1]周金梅,赵文若,建德锋,等.不同品种和栽植密度及整枝方式对黄秋葵产量的影响[J].北方园艺,2013,37(04):34.
[2]刘晓莉,田海娟,钟容,等.盐沉淀法提取紫苏活性蛋白的工艺研究[J].北方园艺,2013,37(04):147.
[3]史艳财,邹 蓉,韦记青,等.黄花倒水莲总黄酮提取工艺研究[J].北方园艺,2014,38(12):134.
　SHI Yan-cai,ZOU Rong,WEI Ji-qing,et al. Study on Extraction Condition of Flavonoid in Polygala fallax[J].Northern Horticulture,2014,38(13):134.
[4]叶 林,李春江,张光弟,等.不同育苗穴数、基质配比和施肥量对温室辣椒幼苗生长及其秧苗质量的影响[J].北方园艺,2014,38(13):50.
　YE Lin,LI Chun-jiang,ZHANG Guang-di,et al.Effects of Different Seedling Number of Holes，Substrates and Fertilization on the Growth and Quality of Greenhouse Pepper Seedlings[J].Northern Horticulture,2014,38(13):50.
[5]张 颖.青蒿素的提取工艺优化研究[J].北方园艺,2014,38(11):122.
　ZHANG Ying.Study on Optimization of the Extraction of Artemisinin[J].Northern Horticulture,2014,38(13):122.
[6]杨申明,王振吉,沈继森.彝药猪鬃草中总黄酮提取工艺研究[J].北方园艺,2014,38(11):125.
　YANG Shen-ming,WANG Zhen-ji,SHEN Ji-sen.Study of the Extraction Technology of Total Flavonoids in Adiantum capillus-veneris[J].Northern Horticulture,2014,38(13):125.
[7]王 嵩,马 杰,赵 维,等.黔西北景天三七高产栽培优化研究[J].北方园艺,2014,38(11):147.
　WANG Song,MA Jie,ZHAO Wei,et al.Research on Optimazation Growing Cutivation of Sedum aizoon L. in Northwest Guizhou[J].Northern Horticulture,2014,38(13):147.
[8]王亚红,王亚丽,祝 波.瓦松总黄酮的索氏提取工艺研究[J].北方园艺,2014,38(10):130.
　WANG Ya-hong,WANG Ya-li,ZHU Bo.Study on Soxhlet Extraction Technology of Total Flavonoids From Orostachys fimbriatus[J].Northern Horticulture,2014,38(13):130.
[9]杨艳俊,王一洋.微波辅助法提取景天三七中齐墩果酸的工艺研究[J].北方园艺,2014,38(10):152.
　YANG Yan-jun,WANG Yi-yang.Study on Extraction Technology of Oleanolic Acid From Sedum aizoon L.[J].Northern Horticulture,2014,38(13):152.
[10]姜 喜,陈加利,肖 巍,等.扁桃茎段增殖培养基的筛选[J].北方园艺,2013,37(22):111.
　JIANG Xi,CHEN Jia-li,XIAO Wei,et al.Screening of the Multiplying Medium of Almond Stem[J].Northern Horticulture,2013,37(13):111.

备注/Memo

第一作者简介：曹小燕（1982-），女，河南开封人，博士，讲师，现主要从事有机化学等研究工作。E-mail：ruoshuizhihan@163.com.

更新日期/Last Update: 2016-08-11