FENG Lijuan,YIN Yanlei,YANG Xuemei,et al.Cloning and Bioinformatical Analysis of DAHPS Gene in Pomegranate Peel[J].Northern Horticulture,2016,40(13):98-103.[doi:10.11937/bfyy.201613026]
石榴果皮DAHPS基因的克隆与生物信息学分析
- Title:
- Cloning and Bioinformatical Analysis of DAHPS Gene in Pomegranate Peel
- Keywords:
- pomegranate; DAHPS gene; cloning; sequence analysis; gallic acid
- 文献标志码:
- A
- 摘要:
- 以‘泰山红’石榴果实为试材，利用RT-PCR技术克隆石榴果皮DAHPS部分基因并对其进行了生物信息学分析。结果表明：获得1 373 bp的DAHPS基因cDNA片段编码393个氨基酸，其氨基酸序列与甜橙（XP_006472576.1）、白梨（XP_009372963.1）、葡萄（NP_001268096.1）、苹果（XP_008386115.1）的相似性分别为94%、93%、92%、92%；预测蛋白质理论分子量约为44 kDa，等电点为9.00，具有Cys-Ser-Ala-His保守区和典型的DAHP_synthe_2超家族保守结构域，为亲水性和跨膜蛋白，不是分泌蛋白；蛋白质二级结构主要由无规则卷曲（40.97%）、α-螺旋（38.17%）、伸展链（12.47%）和β-转角（8.40%）组成。GenBank登录号为KU198932。该研究结果为揭示石榴果实中没食子酸的代谢机理奠定了理论基础。
- Abstract:
- Taking ‘Taishanhong’ pomegranate as test material,the DAHPS gene was cloned by using RT-PCR technology and the bioinformatical characteristics were analyzed.The results showed that the cDNA was 1 373 bp,and it encoded 393 amino acids.The similarities of pomegranate DAHPS gene compared with Citrus sinensis (XP_006472576.1),Pyrus bretschneideri (XP_009372963.1),Vitis vinifera(NP_001268096.1),Malus domestica (XP_008386115.1)were 94%,93%,92% and 92%,respectively.The theoretical molecular mass of DAHPS protein was 44 kDa,and isoelectric point was 9.00.It was hydrophilic and transmembrane protein,but not secreted,which had Cys-Ser-Ala-His and typical DAHP_synthe_2 superfamily conserved domain.The secondary structure of DAHPS protein was composed by random coil(40.97%),α-helix(38.17%),extended strand(12.47%)and β-turn(8.40%).The GenBank accession number was KU198932.The result established a theoretical foundation for revealing the metabolic mechanism of gallic acid in pomegranate fruit.
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备注/Memo
第一作者简介：冯立娟（1982-），女，博士，助理研究员，现主要从事果树遗传资源评价与选育等研究工作。E-mail：fenglj1230@126.com.责任作者：尹燕雷（1976-），男，硕士，副研究员，现主要从事果树遗传资源评价与选育等研究工作。E-mail：yylei66@sina.com.基金项目：山东省自然科学基金资助项目（ZR2014YL022，ZR2015YL056）；山东省果树研究所所长基金资助项目（2013KY04）。