SU Xiuhong,YIN Lei,CHEN Suiqing.Cloning and Expression of cDNA Encoding Key Enzymes(Isopentenyl Diphosphate Isomerase)in Diterpenoids Biosynthesis Pathway From Rabdosia rubescens Henrsl.Hara[J].Northern Horticulture,2016,40(12):80-85.[doi:10.11937/bfyy.201612021]
冬凌草异戊烯基焦磷酸异构酶基因（IDI）克隆与分析
- Title:
- Cloning and Expression of cDNA Encoding Key Enzymes(Isopentenyl Diphosphate Isomerase)in Diterpenoids Biosynthesis Pathway From Rabdosia rubescens Henrsl.Hara
- 关键词:
- 冬凌草; 二萜; 异戊烯基焦磷酸异构酶基因（IDI）
- Keywords:
- Rabdosia rubescens Henrsl.Hara; diterpenoid; IDI
- 文献标志码:
- A
- 摘要:
- 以冬凌草无菌苗为试验材料，在冬凌草转录组信息数据的基础之上，采用逆转录PCR技术克隆到冬凌草二萜类合成的关键酶基因：异戊烯基焦磷酸异构酶基因(isopentenyl diphosphate isomerase，IDI），并对该基因进行了相关的生物信息学分析。结果表明：IDI cDNA基因全长1 050 bp，基因开放阅读框为906 bp，编码301个氨基酸，其蛋白质序列理论分子量为27.4 kDa，等电点为5.06，是一种亲水性蛋白。采用实时荧光定量PCR法分析其组织表达模式，发现IDI在叶中表达量相对较高，在愈伤组织中表达量最低。
- Abstract:
- cDNAs encoding isopentenyl diphosphate isomerase were obtained from the leaves of aseptic seedlings of Rabdosia rubescens by reverse transcription PCR based on the results of transcriptome sequencing.The results showed that the full-length cDNA of IDI was 1 050 bp and contained an open reading frame(ORF)of 906 bp,encoding 301 amino acids.The theoretical molecular weight was 27.4 kDa and the isoelectric point was predicted as 5.06 and it was a kind of hydrophilic protein.Real-time quantitative PCR was used to detect the relative expression levels of of IDI in different tissues of Rabdosia rubescens.And the expression of IDI was relatively high in the leaves,the lowest expression in callus.
参考文献/References:
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相似文献/References:
[1]苏秀红,李庆磊,陈随清,等.冬凌草转录组SSR位点分析及多态性初步评价[J].北方园艺,2016,40(04):93.[doi:10.11937/bfyy.201604025]
SU Xiuhong,LI Qinglei,CHEN Suiqing,et al.Analysis of SSR Loci Information in Transcriptome of Rabdosia rubescens (Henmsl.) Hara and Its Polymorphism[J].Northern Horticulture,2016,40(12):93.[doi:10.11937/bfyy.201604025]
备注/Memo
第一作者简介：苏秀红（1975-），女，博士，副教授，研究方向为中药资源学。E-mail：suxiuhong80@163.com.责任作者：陈随清（1965-），男，博士，教授，现主要从事中药鉴定的教学与科研工作。E-mail：suiqingchen@163.com.基金项目：国家自然科学基金资助项目（81173486）；河南中医学院创新人才资助项目（2011XCXＲC02）；河南省青年骨干教师资助项目（2011GGJS-089）；河南省教育厅科学技术研究重点资助项目（13A360613）。