SHUI Xiaorong,CHEN Can,DING Yuanlin,et al.Study on Problems in the Field of Cordyceps sinensis(Berk.)Sacc Occurrence and Development[J].Northern Horticulture,2016,40(10):184-187.[doi:10.11937/bfyy.201610048]
冬虫夏草发生发育研究中存在的问题研究
- Title:
- Study on Problems in the Field of Cordyceps sinensis(Berk.)Sacc Occurrence and Development
- 文献标志码:
- A
- 摘要:
- 冬虫夏草是产自青藏高原的特色名贵药材,生长于高寒缺氧的极端环境,具有十分重要的经济价值和研究价值。长期以来,有关冬虫夏草的研究一直是学术界的热点,但其发生机理至今未明,也无法实现其人工培植,由于独特的发育特性限制了冬虫夏草产业的持续进展。现结合课题组的长期工作基础,就冬虫夏草发生发育研究中所存在的问题及相关现状进行综述,以期为这一珍贵自然资源的保护和利用提供理论基础。
- Abstract:
- Ophiocordyceps sinensis is a characteristic rare medicinal herbs from the Tibet plateau,which grows in the extreme environment of alpine hypoxia and has very important economic and research value.For a long time,study on Ophiocordyceps sinensis has been the focus of academic circles,but its developmental mechanism remained unclear and is unable to be artificially cultivated,and its related sustainable progress is limited because of the unique developmental characteristics.In this paper,based on long-term work of our team,problems existing in the study of Ophiocordyceps sinensis and present situation were reviewed,in order to provide a theoretical basis for protection and utilization of the precious natural resources.
参考文献/References:
[1]杨大荣.青藏高原冬虫夏草考察纪行[J].大自然,2008(1):36-39. [2]刁治民.青海冬虫夏草资源及生物学特性的初步研究[J].生物学杂志,1996(2):20-22. [3]刘兆红,刘玉玲.玉树州冬虫夏草资源与分布[J].草业与畜牧,2006(12):34-36. [4]刘锡进,郭英兰,俞永信,等.冬虫夏草菌无性阶段的分离和鉴定[J].真菌学报,1989,5(1):35-45. [5]CHEN Y Q,WANG N,ZHOU H,et al.Differentiation of medicinal Cordyceps species by rDNA ITS sequence analysis[J].Planta Med,2002,68(7):635-639. [6]蒲蜇龙,李增智.昆虫真菌学[M].合肥:安徽科学技术出版社,1996. [7]陆龙喜,刘淑梅,时连根.冬虫夏草研究的新进展[J].生物学通报,2002,37(6):4-6. [8]ZHANG Y J,LIU X Z,WANG M.Cloning,expression,and characterization of two novel cuticle-degrading serine proteases from the entomopathogenic fungus Cordyceps sinensis[J].Res Microbiol,2008,159(6):462-469. [9]LEI W,LI S S,ZHANG G R,et al.A real-time qPCR assay to quantify Ophiocordyceps sinensis biomass in Thitarodes larvae[J].J Microbiol,2013,51(2):229-233. [10]雷桅,张古忍,刘昕.钩蝠蛾幼虫寄生冬虫夏草菌潜伏侵染过程的研究[J].环境昆虫学报,2015,37(2):78-82. [11]LEI W,ZHANG G R,PENG Q Y,et al.Development of Ophiocordyceps sinensis through plant-mediated interkingdom host colonization[J].Int J Mol Sci,2015,16(8):17482-17493. [12]曾纬,尹定华,李泉森,等.冬虫夏草菌侵染及寄生阶段的生长发育研究[J].菌物学报,2006,25(4):646-650. [13]齐丽丽.蒲氏蝠蛾不同发育阶段被中国被毛孢寄生的动态研究[D].广州:中山大学,2010. [14]SIGEL M R,LATCH G C M,JOHNSON M C.Fungal endophytes of grasses[J].Annu Rev Phytopathol,2003,25(4):293-315. [15]CARROLL G.Fungal endophytes in stems and leaves:from latent pathogen to mutualistic symbiont[J].Ecology,1988,69(1):2-9. [16]MCLNORY J A,KOEPPER J W.Survey of indigenous bacterial endophytesfrom cotton and sweetcom[J].Plant Soil,1995,173(2):337-342. [17]文才艺,吴元华,田秀玲.植物内生菌研究进展及其存在的问题[J].生态学杂志,2004,23(2):86-91. [18]梁宇,高玉葆.内生真菌对植物生长发育及抗逆性的影响[J].植物学通报,2000,17(1):52-59. [19]张春英.植物的亲密伙伴:菌根[J].林业与生态,2012(2):38. [20]徐丽娟,刁志凯,李岩,等.菌根真菌的生理生态功能[J].应用生态学报,2012,23(1):285-292. [21]IGNOFFO C M,GARCIA C,HOSTETTER D L,et al.Laboratory studies of the entomopathogenic fungus Nomuraea rileyi:Soil-borne contamination of soybean seedings and dispersal of diseased larvae of Trichoplusia ni[J].J Invertebr Pathol,1977,29(2):147-152. [22]WAGNER B,LEWIS L.Colonization of corn,Zea mays,by the entomopathogenic fungus Beauveria bassiana[J].Appl Environ Microb,2000,66(8):3468-3473. [23]ARNOLD A E,LEWIS L C.Ecology and evolution of fungal endophytes and their roles against insects.In:VEGA F and BLACKWELL M eds.Ecological and evolutionary advances in insect-fungus associations[M].Oxford:Oxford University Press,2005. [24]QUESADA-MORAGA E,LANDA B B,MUOZ-LEDESMA J,et al.Endophytic colonisation of opium poppy,Papaver somniferum,by an entomopathogenic Beauveria bassiana strain[J].Mycopathologia,2006,161(5):323-329. [25]AKELLO J,DUBOIS T,GOLD C S,et al.Beauveria bassiana(Balsamo)vuillemin as an endophyte in tissue culture banana(Musa spp.)[J].J Invertebr Pathol,2007,96(1):34-42. [26]POSADA F,AIME M C,PETERSON S W,et al.Inoculation of coffee plants with the fungal entomopathogen Beauveria bassiana(Ascomycota:Hypocreales)[J].Mycol Res,2007,111(Pt 6):748-757. [27]LEI W,CHEN H,ZHANG G R,et al.Molecular identification and food source inference of constructive plants,native to the Ophiocordyceps sinensis Habitat[J].Afr J Biotechnol,2011,10(2):159-167. [28]雷桅.高寒生境中国被毛孢与宿主植物及寄主昆虫依存关系研究[D].广州:中山大学,2012.
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备注/Memo
第一作者简介:税晓容(1985-),女,博士,讲师,研究方向为生物化学与分子生物学。E-mail:shuixiaor@126.com. 责任作者:雷桅(1982-),男,博士,副教授,现主要从事冬虫夏草发育生物学与药理活性评价等研究工作。E-mail:leiwei2006@126.com. 基金项目:国家自然科学基金资助项目(81300035,81403044);广东省公益研究与能力建设专项资助项目(2015A020210097);广东省自然科学基金资助项目(2015A030313520)。