LIAO Xiaoshan,WU Qingqing,ZHANG Zhaojun,et al.Study on 2n Pollen Induction and Its Identification of Lilium oriental[J].Northern Horticulture,2016,40(08):56-60.[doi:10.11937/bfyy.201608016]
东方百合2n花粉诱导及鉴定研究
- Title:
- Study on 2n Pollen Induction and Its Identification of Lilium oriental
- Keywords:
- Lilium oriental ‘Sorbonne’; 2n pollen; colchicine
- 文献标志码:
- A
- 摘要:
- 以东方百合“索尔邦”（Lilium oriental ‘Sorbonne’）的二倍体栽培品种为试材，采用沾有秋水仙素的棉球包裹法处理东方百合“索尔邦”花蕾,探索利用秋水仙素诱导百合2n花粉的方法和可能性，以期得到最佳诱导浓度和时间，并以同株植株叶片为对照，对2n花粉粒采用细胞流式仪鉴定。结果表明：在花粉细胞进行减数分裂中期I时，采用包裹法处理花蕾最高诱变率达68%，其最适秋水仙素浓度为0.4%，处理时间为24 h。配子形成过程中出现四分体时期发现二分体、三分体及产生胞质分离而染色体不分离异常等情况。鉴定结果证实所获得2n花粉为二倍体。表明了人工诱导2n花粉是产生百合有性多倍化的一种有效途径。
- Abstract:
- Lilium oriental ‘Sorbonne’ diploid cultivars were used as material to explored the method and possibility of stained with colchicine package method to induce 2n gamete.In order to get the best induced concentration and time,taking leaf from the same plant as control,2n pollen were authenticated by flow cytomety instrument.The results showed that the highest mutation rate of flower buds was 68%,the best concentration of colchicines was 0.4%，and the handling time was 24 hours in the meiosis stage I of the pollen mother cell through package method.The abnormal phenomena like days and triads at the tetrad stage and cytoplasm separation but the chromosome non separation，etc.were observed,which was identificated by flow cytometry.The results obtained that the 2n pollen was diploid as the monoecious plants for the control.The results implied that 2n pollen induced artificially can be a powerful method to creat the sexual polyploids of Lilium oriental.
参考文献/References:
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备注/Memo
第一作者简介：廖晓珊（1984-），女，湖南长沙人，硕士，农艺师，现主要从事百合杂交育种技术等研究工作。E-mail：48191033@qq.com. 责任作者：郑思乡（1966-），男，湖南华容人，博士，研究员，现主要从事花卉生物技术与育种等研究工作。E-mail：zhengqianlian@163.com. 基金项目：国家自然科学基金资助项目（31160176）；株洲市企业创新引导资金资助项目（株科发[2014]45号）；株洲市领军人才资助项目；贵州省科技厅资助项目（黔科合J字[2010]2090；黔科合NY字[2010]3074）。