LIU Ping,LYU Baohe,DING Yifeng,et al.Effects of 1-hexyl-3-methylimidazolium Bromide Ionic Liquid on Seed Germination and Seedling Growth of Cucumber[J].Northern Horticulture,2016,40(08):1-5.[doi:10.11937/bfyy.201608001]
离子液体1-己基-3-甲基咪唑溴化盐对
- Title:
- Effects of 1-hexyl-3-methylimidazolium Bromide Ionic Liquid on Seed Germination and Seedling Growth of Cucumber
- 文献标志码:
- A
- 摘要:
- 以黄瓜“绿优四号”品种为试材，研究了不同浓度（0、5、10、15、20、25、30 mg/L）的离子液体1-己基-3-甲基咪唑溴化盐（［C6mim］Br）对其种子萌发、幼苗形态和叶片生理生化的影响。结果表明：不同浓度的离子液体［C6mim］Br均不同程度地降低了黄瓜种子的发芽率和发芽势，减小了幼苗的根长和茎长，使幼苗叶片中脯氨酸（Pro）和叶绿素(Chl)含量下降，并且上述抑制效应具有典型的剂量依赖性特点；随着离子液体［C6mim］Br浓度的升高，黄瓜幼苗叶片中的超氧化物歧化酶（SOD）和过氧化物酶（POD）活力均先升后降，而超氧阴离子自由基（O?[TX-*4]2）产生速率和丙二醛（MDA）含量则与保护酶活力呈相呼应的先降后升变化；低浓度的离子液体［C6mim］Br可启动保护酶系统的应激保护反应，在一定程度上缓解离子液体［C6mim］Br的毒害作用，但是其调节能力有限，当离子液体浓度>15 mg/L时，随着其浓度的升高，保护酶的活力下降加剧。
- Abstract:
- With cucumber ‘Lyuyou Fourth’ as test material，effect of different concentration of ionic liquids（0，5，10，15，20，25，30 mg/L）1-hexyl-3-methyl imidazole bromide salts([C6mim]Br)influence on seed germination，seedling morphology，physiological and biochemical characteristics of leaf were studied.The results showed that ionic liquid [C6mim]Br reduced the cucumber seed germination rate and germination potential in different levels,decreased the seedling root and stem length，reduced proline (Pro) and chlorophyll content of seedling leaf，and the above inhibitory effect should have the characteristics of a typical dose dependent.With the ionic liquid [C6mim]Br concentration increased，superoxide dismutase (SOD) and peroxidase (POD) vitality in cucumber seedling leaves all increased at first then decreased.Echoing each other at a distance，superoxide anion radicals (O?[TX-*4]2) production rate and malondialdehyde (MDA) content decreased at first and then rose.Low concentration of [C6mim]Br could start stress protective reaction of protective enzyme system，alleviate the ionic liquid [C6mim]Br’s toxic effects in a certain degree，but its ability to regulate was limited，when the concentration of ionic liquids>15 mg/L of ionic liquids，with the increase of ionic liquid concentration，protective enzyme vigor declined intensively.
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备注/Memo
第一作者简介：刘萍（1958-），女，河南潢川人，本科，教授，现主要从事植物生理学等教学及科研工作。E-mail：Liuping5812@sina.com.基金项目：河南省重点科技攻关计划资助项目（152102110081，122102310356）。