[1]包艳存,李书华,李保华,等.芦笋“冠军”亲本组培快繁技术研究[J].北方园艺,2016,40(07):98-103.[doi:10.11937/bfyy.201607025]
　BAO Yancun,LI Shuhua,LI Baohua,et al.Study on Tissue Culture and Rapid Propagation Technique of Parental Asparagus officinial L. of ‘Champion’[J].Northern Horticulture,2016,40(07):98-103.[doi:10.11937/bfyy.201607025]

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芦笋“冠军”亲本组培快繁技术研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第40卷期数: 2016年07 页码: 98-103 栏目: 出版日期: 2016-04-15

Title:: Study on Tissue Culture and Rapid Propagation Technique of Parental Asparagus officinial L. of ‘Champion’

作者:: 包艳存; 李书华; 李保华; 郑红霞; 牟萌; 厉广辉; （山东省潍坊市农业科学院生物所，山东潍坊 261041）

Author(s):: BAO Yancun; LI Shuhua; LI Baohua; ZHENG Hongxia; MU Meng; LI Guanghui; (Biotechnology Institute，Weifang Agricultural Science Institute,Weifang，Shandong 261041)

关键词:: 芦笋; “冠军”; 茎尖组培; 过渡移栽

Keywords:: Asparagus officinial L.; ‘Champion’; shoot tip tissue; transplant

DOI:: 10.11937/bfyy.201607025

文献标志码:: A

摘要:: 以芦笋“冠军”父、母本为试材，采用不同浓度NAA、6-BA、KT、IBA激素配比，进行启动、增殖、生根、过渡培养试验，研究了适宜其父、母本茎尖快繁的培养基。结果表明：MS+NAA 0.05 mg/L+6-BA 0.5 mg/L+KT 0.1 mg/L为母本启动培养基，MS+NAA 0.10 mg/L+6-BA 0.5 mg/L+KT 0.2 mg/L为父本启动培养基；MS+NAA 0.1 mg/L+6-BA 1.5 mg/L+KT 0.1 mg/L为母本继代增殖培养基，MS+NAA 0.2 mg/L+6-BA 1.5 mg/L+KT 0.05 mg/L为父本继代增殖培养基；1/2MS+NAA 0.05 mg/L+6-BA 1.5 mg/L+KT 1.5 mg/L+IBA 1.5 mg/L为母本生根培养基，1/2MS+NAA 0.1 mg/L+6-BA 1.5 mg/L+KT 1.0 mg/L+IBA 2.0 mg/L为父本生根培养基，父、母本在适宜的生根培养基上，均生出8条以上的第Ⅰ类型根，生根率达95%以上,并建立了适宜的过渡移栽技术规程，过渡移栽成活率达95%以上。

Abstract:: Taking parental Asparagus officinial of ‘Champion’ as materials，using different hormone combination of NAA,6-BA,KT,IBA,the start，proliferation，rooting，transition culture experiments were conducted and the suitable shoot-tip propagation medium for the parents were selected.The results showed that MS+NAA 0.05 mg/L+6-BA 0.5 mg/L+KT 0.1 mg/L as the start medium for the female parent；MS+NAA 0.10 mg/L+6-BA 0.5 mg/L+KT 0.2 mg/L as the start medium for the male parent；MS+NAA 0.1 mg/L+6-BA 1.5 mg/L+KT 0.1 mg/L as the subculture medium for the female parent；MS+NAA 0.2 mg/L+6-BA 1.5 mg/L+KT 0.05 mg/L as the subculture medium for the male parent；1/2MS+NAA 0.05 mg/L+6-BA 1.5 mg/L+KT 1.5 mg/L+IBA 1.5 mg/L as the rooting medium for the female parent；1/2 MS+NAA 0.1 mg/L+6-BA 1.5 mg/L+KT 1.0 mg/L+IBA 2.0 mg/L as the rooting medium for the male parent.In the appropriate rooting medium，the parents gave birth to eight or more roots of type I.Rooting rate and transplant survival rate were over 95%.

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备注/Memo

第一作者简介：包艳存（1979-），女，硕士，农艺师，研究方向为芦笋栽培育种。E-mail:bycnky@163.com.

更新日期/Last Update: 2016-06-15