WU Hao,GAO Yong,DU Meie,et al.Characteristics of Chlorophyll Fluorescence Parameters in Forsythia suspensa（Thunb.）Vahl Under NaCl Stress[J].Northern Horticulture,2016,40(07):55-60.[doi:10.11937/bfyy.201607016]
盐胁迫对连翘叶绿素荧光参数的影响
- Title:
- Characteristics of Chlorophyll Fluorescence Parameters in Forsythia suspensa（Thunb.）Vahl Under NaCl Stress
- 文献标志码:
- A
- 摘要:
- 以2年生连翘（Forsythia suspense(Thunb.)Vahl）扦插幼苗为试材，采用温室盆栽的方法，利用便携式脉冲调制叶绿素荧光仪测定了不同质量浓度（0.00%、0.20%、0.40%和0.60%）NaCl胁迫下连翘叶片各项叶绿素荧光诱导动力学参数变化特征。结果表明：随着盐分胁迫的加重，连翘叶片的PSII最大光化学效率（Fv/Fm）、光化学荧光淬灭系数（qP）值呈降低的趋势，且重度胁迫下连翘Fv/Fm、qP值与其它处理间差异显著（P＜0.05）。从中度盐分胁迫开始，连翘PSⅡ反应中心开放程度减小，光合特性显著降低；而连翘的Y(NPQ)随着盐胁迫的加剧随之增大，重度胁迫下达到峰值，在盐分胁迫过程中连翘叶片的表观光合电子传递速率（ETR）在轻、中度胁迫与CK间差异不显著（P＞0.05）；Fv/Fm与ETR呈极显著正相关（P＜0.01），ФPSⅡ与Y(NPQ)呈显著负相关（P＜0.05），qP与qN和ETR分别呈极显著负相关，ETR与Y(NO)呈显著负相关。
- Abstract:
- With no damage of the leaf fluorescence diagnosis technology for the research of forsythia photosystem response under salinity stress，to provide reference for urban greening and water and soil conservation of forsythia in saline area of northern China.With 2 years of raw fructus forsythiae （Forsythia suspense(Thunb.)Vahl）cuttage seedlings as experimental material，adopted the method of greenhouse potting，used portable pulse modulation chlorophyll fluorescence spectrometer to determine each chlorophyll fluorescence induction kinetics parameters variation characteristics of forsythia blade under different quality concentration of NaCl stress (0.00%，0.20%，0.40% and 0.20%).The results showed that with the increase of salt stress，the maximum PSII photochemical efficiency (Fv/Fm)，the largest photochemical fluorescence quenching coefficient (qP) value in blades of forsythia appeared the tendency of decrease，and under severe stress，the difference between forsythia Fv/Fm，qP value and other among treatments were significant (P＜0.05)，from the beginning of the moderate salinity stress，the degree of reaction center of forsythia PSⅡ opening was reduced，the photosynthetic characteristics were significantly reduced；while Y(NPQ) of forsythia increased with the salinity stress intensifying，when the severe stress up to peak value，the ETR of forsythia blades in the process of salinity stress，which had no significant difference under mild and moderate stress between the CK (P>0.05)；Fv/Fm and ETR showed very significant positive correlation (P＜ 0.01)，ФPSⅡ and Y(NPQ) appeared significant negative correlation (P＜0.05)，and qP and qN had very significant negative correlation with ETR respectively，ETR and Y(NO) had significant negative correlation.In conclusion，it considered that leaf fluorescence technology could be widely used in salt-tolerant plants screening.
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备注/Memo
第一作者简介：吴昊（1989-），男，硕士研究生，研究方向为荒漠化防治。E-mail：wh3816@163.com.责任作者：高永（1962-），男，博士，教授，博士生导师，研究方向为荒漠化防治。E-mail：13948815709@163.com.基金项目：引进国际先进林业科学技术资助项目（2015-4-22）；内蒙古自治区自然科学基金资助项目（2015MS0301）。