LU Yanxin,WANG Xue,QU Baihong.Clone and Sequence Analysis of PyMYB114 Gene cDNA in Pyrus bretschneideri Rehd. ‘Pingguoli’[J].Northern Horticulture,2016,40(06):89-91.[doi:10.11937/bfyy.201606022]
“苹果梨”PyMYB114基因cDNA全长的克隆与序列分析
- Title:
- Clone and Sequence Analysis of PyMYB114 Gene cDNA in Pyrus bretschneideri Rehd. ‘Pingguoli’
- 文献标志码:
- A
- 摘要:
- 以延边“苹果梨”果实为试材,采用同源克隆的试验方法,研究了果实着色过程的转录基因PyMYB114,并对其进行生物信息学分析。结果表明:PyMYB114的cDNA全长为744 bp,其包含1个完整的编码201〖KG-*4〗个氨基酸的ORF,其编码蛋白的分子量约为23.48 kDa,理论等电点(pI)为9.57。同源性分析表明,PyMYB114〖KG-*4〗基因与白梨的MYB114基因核酸序列的相似性高达99%。系统进化树表明,PyMYB114编码的氨基酸和白梨的MYB114编码的氨基酸在进化上亲缘关系最近。
- Abstract:
- Taking the Pyrus bretschneideri Rehd. ‘Pingguoli’ in Yanbian as test material,transcription gene PyMYB114 during the pigmentation of ‘Pingguoli’ were researched by using homologous cloning and bioinformatics technology.The results showed that the cDNA of PyMYB114 was 744 bp in length,encoded 201 amino acids with a calculated molecular weight(MV) of 23.48 kDa and isoelectric point(pI) of 9.57.Homology analysis revealed that the similarity between these cDNA fragments and the genes of other rosaceous plants was up to 99%.Phylogenetic tree indicated that amino acids encoded by PyMYB114 and Pyrus bretschneideri were the most closed on the evolution.
参考文献/References:
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备注/Memo
第一作者简介:鹿艳新(1988-),女,硕士研究生,研究方向为果树生理学。E-mail:395297341@qq.com.责任作者:曲柏宏(1963-),男,博士,教授,博士生导师,现主要从事园艺植物栽培与生理等研究工作。E-mail:bhqu211@ybu.edu.cn. 基金项目:国家自然科学基金资助项目(31160389)。