LU Jinghui.RAPD Analysis of the Genetic Diversity of Calendula officinalis Cultivated in Yunnan Province[J].Northern Horticulture,2016,40(05):123-127.[doi:10.11937/bfyy.201605033]
云南栽培金盏花遗传多样性RAPD分析
- Title:
- RAPD Analysis of the Genetic Diversity of Calendula officinalis Cultivated in Yunnan Province
- Keywords:
- Calendula officinalis; RAPD; genetic diversity
- 文献标志码:
- A
- 摘要:
- 用采自云南昆明、曲靖和玉溪等地的8份金盏花种质资源为试材，利用改良CTAB法提取DNA，筛选引物，优化PCR反应体系，对其进行遗传多样性RAPD (random amplified polymorphic DNA)和NTSYS2聚类分析，以建立金盏花亲缘关系分析方法，明确分布在云南省各地的金盏花种质资源之间的亲缘关系和遗传多样性。结果表明：优化的PCR反应体系能达到良好的扩增效果，30条RAPD引物在8份金盏花种质资源中共产生165个位点，其中45个位点具有遗传多态性，约占27.28%。CK与7号的相似性最小，用CK和7号作为亲本的后代会有较大的分离。试验表明金盏花种植资源遗传多样性丰富，遗传关系与其地理关系密切相关，但并非严格的限聚在一起，需结合表型更深入地分析。
- Abstract:
- Taking 8 marigold germplasm resources collected in Yunnan Province as materials，the DNA was extracted by modified CTAB method.The PCR reaction system was built and the primers were selected to establish a method for analyzing the genetic relationship by RAPD and NTSYS2.The results showed that the optimal reaction system could achieve better amplified effect.A total of 165 loci were obtained，of which 45 loci were polymorphic,accounting for about 27.28%.The similarity between CK and No.7 was minimum.The test showed that the genetic diversity of marigold germplasm resources in Yunnan were abundant in Yunnan Province,and relationship between genetic and geographical relationship were closely related with each other,but not strictly limited together,and more in-depth analysis of phenotype should be combined.
参考文献/References:
[1]金敬宏，张卫明，孙晓明，等.金盏花的栽培和经济用途[J].中国野生植物资源，2003(4)：40-41. [2]赵继荣，张肖凌，雒淑珍，等.不同授粉次数和环境条件对金盏花结实率的影响[J].广西农业科学，2010，41(3)：250-252. [3]赵继荣，雷耀湖，何庆祥，等.水氮耦合对金盏花产量和叶黄素含量变化的影响[J].南方农业学报，2011(8)：923-925. [4]刘爱荣，张远兵，方园园，等.盐胁迫对金盏菊生长、抗氧化能力和盐胁迫蛋白的影响[J].草业学报，2011(6)：52-59. [5]田海燕，王平，沈向群，等.万寿菊W205雄性不育两用系的遗传及植物学特征研究[J].北方园艺,2007(2)：105-107.〖HJ〗 [6]胡裕清，赵树进.RAPD技术及其在植物研究中的应用[J].生物技术通报,2010(5)：74-77. [7]沈一岚，续九如，李福荣，等.万寿菊RAPD分子标记优化体系的建立[J].吉林林业科技,2006(1)：10-14. [8] WILLIAMS J G K，KUBELIK A Ｒ，LIVAK K J，et al.DNA Polymerphisms amplified by arbimary primers are useful as genetic morkors[J]．Nucleic Acids Ｒesearch，1990(18)：6531-6534. [9]NEI M，LI W H.Mathematical model for studying genetical variation in terms of restriction endonucleases[J].Proceedings of the National Academy of Sciences of the USA,1979，74:5267-5273. [10]戴思兰，陈俊愉，李文彬.菊属植物RAPD反应体系的建立[J].北京林业大学学报，1996(1)：47-52. [11]徐文斌，郭巧生，王长林.药用菊花遗传多样性的RAPD分析[J].中国中药杂志，2006(1)：18-21. [12]孙维洋，李剑芳，邱进，等.单叶蔓荆种质资源遗传多态性的RAPD分析[J].中国医药导报,2011，8（33）：5-7. [13]汤志成,陈璇,张庆滢，等.野生大麻种质资源表型及其ＲAPD 遗传多样性分析[J].西部林业科学,2013,42(3):61-66. [14]胡尊红,郭鸿彦,胡学礼，等.大麻品种遗传多样性的 AFLP 分析[J].植物遗传资源学报,2012,13(4)：555-561.
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备注/Memo
作者简介：鲁京慧（1967-)，女，河北承德人，本科，实验师，现主要从事园林工程及园林史的教学与科研工作。E-mail：lsz520999@126.com.