YANG Yan,JIANG Lijuan,TANG Yuxi,et al.Organogenesis and Regeneration of Symplocos paniculata From Immature Zygotic Embryos in vitro[J].Northern Horticulture,2016,40(05):119-123.[doi:10.11937/bfyy.201605032]
Organogenesis and Regeneration of Symplocos paniculata From Immature Zygotic Embryos in vitro
- Title:
- Organogenesis and Regeneration of Symplocos paniculata From Immature Zygotic Embryos in vitro
- 文献标志码:
- A
- 摘要:
- Taking immature zygotic embryos of Symplocos paniculata as materials，using modified MS medium,the organogenesis and regeneration were researched.The results showed that the mMS medium could induce callus greatly，the highest induction rate 92.5% was obtained on the medium with 0.2 mg/L 6-BA+0.1 mg/L NAA.72.4% of calluses initiated green bud-like structures when they were cultured on mMS plus 0.25 mg/L 6-BA+0.15 mg/L NAA.The calluses with green bud-like structures were subcultured onto mMS medium without plant growth regulators for 15 days，76.6% of them developed into shoots.Shoots elongated to 1.5 cm long on 1/2 mMS medium plus 1.5% sucrose 20 days after culture.
- Abstract:
- Taking immature zygotic embryos of Symplocos paniculata as materials，using modified MS medium,the organogenesis and regeneration were researched.The results showed that the mMS medium could induce callus greatly，the highest induction rate 92.5% was obtained on the medium with 0.2 mg/L 6-BA+0.1 mg/L NAA.72.4% of calluses initiated green bud-like structures when they were cultured on mMS plus 0.25 mg/L 6-BA+0.15 mg/L NAA.The calluses with green bud-like structures were subcultured onto mMS medium without plant growth regulators for 15 days，76.6% of them developed into shoots.Shoots elongated to 1.5 cm long on 1/2 mMS medium plus 1.5% sucrose 20 days after culture.
参考文献/References:
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备注/Memo
一作者简介：杨艳（1980-），女，博士，助理研究员，研究方向为林木选育与栽培。E-mail：yangyanzupei@126.com.责任作者：蒋丽娟（1965-），女，博士，教授，现主要从事能源植物培育与利用等研究工作。E-mail：liljiang1965@sina.com.基金项目：长沙市科技计划资助项目（kl307012-21）。