WANG Xue,QU Baihong,LU Yanxin,et al.Molecular Cloning and Expression of PyANS in ‘Pingguoli’ During the Pigmentation[J].Northern Horticulture,2016,40(05):108-112.[doi:10.11937/bfyy.201605030]
苹果梨果皮花色素苷合成相关基因PyANS的克隆与表达分析
- Title:
- Molecular Cloning and Expression of PyANS in ‘Pingguoli’ During the Pigmentation
- 关键词:
- 苹果梨; 花色素苷合成相关基因; 基因克隆; 序列分析; 表达分式
- Keywords:
- ‘Pingguoli’; PyANS; gene cloning; sequence analysis; expression patterns
- 文献标志码:
- A
- 摘要:
- 以延边地区苹果梨为试材，采用同源克隆及实时荧光定量PCR的试验方法，研究PyANS基因在苹果梨解袋前后果实着色过程中的表达特性及与花青苷积累的关系。结果表明：PyANS的全长cDNA为1 075 bp，其开放阅读框（ORF）编码358个氨基酸，分子量约为40 kDa，理论等电点pI为5.38。同源性分析表明与同属的砂梨相似性高达99%，实时荧光定量PCR分析表明PyANS基因受到光诱导表达量迅速上升，去袋1 d后表达量迅速增加，第10天时达到最大值，随后迅速下降，最终稳定在同一表达水平，与果实着色和花色苷含量测定的结果相对应，表明PyANS基因对果实花色素苷积累有一定的促进作用。
- Abstract:
- With the ‘Pingguoli’ debagged and not sack as test material,the full-length cDNA sequences of PyANS was cloned by homologous cloning technology and real-time fluorescent quantitative PCR technique,then to analyze the relationship between the expression and the accumulation of anthocyanin during the pigmentation of ‘Pingguoli’.Sequence analysis showed that the cDNA of PyANS was 1 075 bp in length,encoded 358 amio acids with a calculated molecular weight(MV) of 40 kDa and isoelectric point(pI) of 5.38.Homology analysis revealed that the similarity between these cDNA fragments and the genes of other rosaceous plants was up to 99%.Real-time fluorescent quantitative PCR indicated the expression levels of PyANS was markedly enhanced in the sunlit side,increased rapidly on the first day after bagging,then reached a maximum value at 10th day and started to decline quickly,however,expressed stably in the same level finally.The color code and anthocyanins content produced results essentially in agreement with these figures.In addition that the PyANS had a certain role in promoting the accumulation of fruit anthocyanin.
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备注/Memo
第一作者简介：王雪(1990-)，女，硕士研究生，研究方向为果树生理与分子研究。E-mail:18643165303@163.com.责任作者：曲柏宏（1963-），男，博士，教授，博士生导师，现主要从事园艺植物栽培与生理等研究工作。E-mail:bhqu211@ybu.edu.cn.基金项目：国家自然科学基金资助项目（31160389）。