[1]李许真,姜永华,陈书霞.AM真菌和根结线虫互作对黄瓜幼苗生理变化的影响[J].北方园艺,2016,40(05):9-13.[doi:10.11937/bfyy.201605003]
　LI Xuzhen,JIANG Yonghua,CHEN Shuxia.Interaction Between AM Fungi and Root-knot Nematode on the Physiological Characteristics of Cucumber Seedlings[J].Northern Horticulture,2016,40(05):9-13.[doi:10.11937/bfyy.201605003]

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AM真菌和根结线虫互作对黄瓜幼苗生理变化的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第40卷期数: 2016年05 页码: 9-13 栏目: 出版日期: 2016-03-15

Title:: Interaction Between AM Fungi and Root-knot Nematode on the Physiological Characteristics of Cucumber Seedlings

作者:: 李许真; 姜永华; 陈书霞; (西北农林科技大学园艺学院,农业部西北地区园艺作物生物学与种质创制重点实验室，陕西杨凌 712100)

Author(s):: LI Xuzhen; JIANG Yonghua; CHEN Shuxia; (College of Horticulture，Northwest Agriculture and Forestry University/Key Laboratory of Horticultural Plant Germplasm Resources Utilization in Northwest China，Yangling,Shaanxi 712100)

关键词:: 黄瓜; 丛枝菌根真菌; 根结线虫; 酶活性

Keywords:: cucumber; Arbuscular mycorrhiza; Meloidogyne sp.; enzyme activity

DOI:: 10.11937/bfyy.201605003

文献标志码:: A

摘要:: 以黄瓜为试材，采用盆栽方法，设置了播种30 d后接种根结线虫(M.)、接种G.i 30 d后接种根结线虫(G.i+M.)和接种G.v 30 d后接种根结线虫（G.v+M.）3个处理，研究了2种AM真菌Glomus inartradiees(G.i)和Glomus versiforme(G.v)与根结线虫Meloidogyne sp.(M.)互作对黄瓜幼苗生理变化的影响。结果表明：黄瓜幼苗受到根结线虫侵染后，叶片的PAL、PPO活性及总酚含量分别在144、72、288 h达到最高；接种AM真菌提高了黄瓜幼苗PAL、PPO活性及多酚含量，表明接种AM真菌可提高黄瓜幼苗对根结线虫侵染的抗性。接种G.v和G.i真菌均使黄瓜幼苗MDA含量低于对照，降低了后期根结线虫侵染造成的膜脂过氧化伤害。综合以上分析可知，接种AM真菌G.v和G.i不仅激发了黄瓜幼苗较高的保护性酶活性，且激发了黄瓜幼苗的次生代谢物质总酚的积累，增加了植株对根结线虫的抗性，同时维护了细胞膜的完整性。G.v对黄瓜植株的保护作用要优于G.i。

Abstract:: Taking cucumber as material,the cucumber seedlings were inoculated with root-knot nematode (Meloidogyne sp.) after sowing 30 days，pre-inoculated with Glomus versiforme and then inoculated with Meloidogyne sp.(G.v+M.)，and pre-inoculated with Glomus inartradiees and then inoculated with Meloidogyne sp.(G.i+M.) separately，and normal cultivation as control.The effect of interaction between AM fungi and root-knot nematode on physiological changes was conducted based on the pot cultivation cucumber seedlings.The results showed that the activities of PAL，PPO and contents of polyphenol reached the highest at 144 h，72 h，288 h respectively after inoculating with root-knot nematode;the activity of PAL and PPO and contents of polyphenol increased in the treatments which inoculated with AM fungi.It showed that the resistance to root-knot nematode was enhanced because of inoculating with AM fungi.The content of MDA in the treatments inoculated with AM fungi was lower than that of control and the damage of plasmalemma peroxide caused by Meloidogyne sp. decreased.In one word，the higher PAL and PPO activities of the cucumber seedlings were elicited，and the polyphenol substances accumulated after inoculating with AM fungi,the resistance of Meloidogyne sp. was increased,cell membrane integrity was maintained.The protect effect of G.v was better than that of G.i on cucumber seedlings.

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备注/Memo

第一作者简介：李许真(1989-)，女，硕士研究生，研究方向为蔬菜生理与生物技术。E-mail：15209276286@163.com.责任作者：陈书霞(1971-)，女，博士，教授，博士生导师，研究方向为蔬菜生理与生物技术。E-mail：shuxiachen@nwsuaf.edu.cn.基金项目：2013年陕西省县域重点科技资助项目(K332021408)；杨凌示范区科技计划资助项目(2014NY-34)；陕西省农业科技计划攻关资助项目(2013K02-04)。

更新日期/Last Update: 2016-04-25