ZHANG Xuehua,LI Linling,CHENG Hua,et al.Characterizing a Novel Strain of Bacillus amyloliquefaciens CMN1308 for Potential Biological Control in Pathogenic Bacterium[J].Northern Horticulture,2016,40(02):117-121.[doi:10.11937/bfyy.201602031]
解淀粉芽孢杆菌CMN1308抗真菌特性研究
- Title:
- Characterizing a Novel Strain of Bacillus amyloliquefaciens CMN1308 for Potential Biological Control in Pathogenic Bacterium
- Keywords:
- C.mollissima; biological control; antifungal spectrum; stability
- 文献标志码:
- A
- 摘要:
- 以解淀粉芽孢杆菌CMN1308为试材，采用平板对峙培养法，测定CMN1308对葡枝根霉(Rhizopus stolonifer)、腐皮镰刀菌(Fusarium solani)、黑葡萄穗霉(Stachybotrys chartarum)、栗疫菌(Cryphonectria parasitica)、可可毛色二孢菌(Lasiodiplodia theobromae)、扩展青霉(Penicillium expansum)、黑曲霉(Aspergillus niger)的抑菌效果，并进行了镜检观测及无菌滤液中抑菌成分稳定性测定，以充分研究其抑菌效果及抗真菌特性。结果表明：拮抗菌CMN1308对上述7种供试病原菌均有较强的抑制作用，表现出抑菌谱广的特点；其中对扩展青霉的抑制作用最好，抑菌圈直径可达27.1 mm；CMN1308对可可毛色二孢菌菌丝生长有较强的抑制作用，且其发酵产生的抑菌物质热稳定性较好，耐碱怕酸，对紫外线不敏感。这为更进一步探索板栗采后生防保鲜技术和微生物制剂的开发与应用提供了一定的理论与技术支持。
- Abstract:
- Taking Bacillus amyloliquefaciens CMN1308 as materials，with the method of confront culture，the antibiosis effect on Rhizopus stolonifer，Fusarium solani，Stachybotrys chartarum,Cryphonectria parasitica，Lasiodiplodia theobromae，Penicillium expansum and Aspergillus niger was studied.The results showed that CMN1308 had stronger antibiosis effect on the seven major pathogens of chestnut.Among this，CMN1308 had a best biocontrol to P.expansum,the inhibition zone diameter was 27.1 mm.The further study on the stability of antimicrobial components in CMN1308 showed，the growth of P.expansum hyphal was limited by the antimicrobial components isolated from CMN1308，which also had a good stability to hot temperature，alkali and UV，but sensitive to acid.The results suggested that antibiotic effect and inducible resistance were the major mechanism of CMN1308 on chestnut pathogenic.The finding of this study would help to optimize the practical use of CMN1308 in the biological control of chestnut or other fruit rot caused by pathogenic fungi.
参考文献/References:
[1]汪永文，张良富，王力，等.板栗贮藏保鲜问题及解决措施[J].经济林研究，2009，27(3)：108-114. [2]KEIJITSU T，ATSUSHI I，HIROMITSU N.Isolation of anteiso-C17，iso-C17，iso-C16，and iso-C15 Bacillomycin D from Bacillus amyloliquefaciens SD-32 and their antifungal activities against plant pathogens[J].Journal of Agricultural and Food Chemistry，2014，62:1469-1476. [3]CHEN D，LIU X，LI C Y，et al.Isolation of Bacillus amyloliquefaciens S20 and its application in control of eggplant bacterial wilt[J].Journal of Environmental Management，2014，137：120-127. [4]ROMY S，JOACHIM V，ANTO B,et.al.Amylocyclicin，a novel circular bacteriocin produced by Bacillus amyloliquefaciens FZB42[J].Journal of Bacteriology，2014，196(10)：1842-1852. [5]王英国，王军华，权春善，等.解淀粉芽孢杆菌抗菌活性物质的分离纯化及抑菌活性研究[J].中国生物工程杂志，2007，27(12)：41-45. [6]黎波，魏学军，李亚宁，等.解淀粉芽孢杆菌X-278抗菌蛋白初步分析[J].河南农业科学，2013，42(7)：68-71，83. [7]张艳群，来航线，韦小敏，等.两株生防芽孢细菌筛选、鉴定及拮抗研究[J].微生物学通报，2014，41(2)：281-289. [8]陈莉，米娜瓦尔，公勤，等.生防细菌A178的分子鉴定及抑菌机理的研究[J].中国农学通报，2009，25(22)：241-244. [9]杨振.枯草芽孢杆菌对油桃采后病害的生物防治及防治机理研究[D].天津：天津科技大学，2009. [10]臧威，何旭，孙剑秋，等.桦木内生真菌的分离与代谢产物的抑菌活性[J].生态环境学报，2012，21(4)：661-665. [11]郝建安，曹志辉，赵凤梅，等.解淀粉芽孢杆菌NK10.BAhjaWT 抑真菌作用的研究[J].微生物学通报，2008，35(6)：903-908. [12]布婷婷，周国英，刘君昂.油茶内生拮抗细菌 Y13 菌株 16S rDNA序列分析及抑菌机理研究[J].经济林研究，2012，30(3)：11-15. [13]张宝俊，张家榕，韩巨才，等.内生解淀粉芽孢杆菌LP-5抗菌蛋白的分离纯化及特性[J].植物保护学报，2010，37(2)：143-147. [14]洪鹏，安国栋，胡美英，等.解淀粉芽孢杆菌防治果蔬采后病害研究进展[J].中国农学通报，2013，29(12)：168-173.
相似文献/References:
[1]程 华,李琳玲,袁红慧,等.内源激素含量变化与板栗花芽分化关系研究[J].北方园艺,2013,37(22):5.
CHENG Hua,LI Lin-ling,YUAN Hong-hui,et al.Study on the Relationship of Changes of the Endogenous Hormone Content in Castanea mollissima and Bud Differentiation[J].Northern Horticulture,2013,37(02):5.
[2]李琳玲,程 华,吴聪华,等.SPE-HPLC法同时分离测定板栗花芽中四种内源激素的研究[J].北方园艺,2014,38(01):22.
LI Lin-ling,CHENG Hua,WU Cong-hua,et al.Simultaneous Determination of 4 Endogenous Hormones in Chinese Chestnut Floral Bud by Solid Phase Extraction-High Performance Liquid Chromatography[J].Northern Horticulture,2014,38(02):22.
[3]鲁海菊,王 波,潘柳君,等.深绿木霉P3.9生防菌株固体发酵条件优化筛选[J].北方园艺,2014,38(14):119.
LU Hai-ju,WANG Bo,PAN Liu-jun,et al.Screening on Conditions of Solid Fermentation for Biocontrol Strain P3.9 of Trichoderma atroviride [J].Northern Horticulture,2014,38(02):119.
[4]熊仕俊,孙成龙,施闯,等.番茄青枯病菌拮抗放线菌的筛选及鉴定[J].北方园艺,2014,38(05):114.
XIONG Shi-jun,SUN Cheng-long,SHI Chuang,et al.Screening and Identifying of Antagonistic Actinomycetes Against Ralstonia solancearum[J].Northern Horticulture,2014,38(02):114.
[5]刘国彬,兰彦平,曹均,等.板栗和锥栗远缘杂交亲和性研究[J].北方园艺,2013,37(15):1.
LIU Guo-bin,LAN Yan-ping,CAO Jun,et al.Study on the Distant Hybridization Compatibility Between Castanea mollissima and C.henryi[J].Northern Horticulture,2013,37(02):1.
[6]高 宇.茶尺蠖天敌生态学研究进展[J].北方园艺,2014,38(09):203.
GAO Yu.Research Progress on Ecology of Natural Enemies of Tea Geometrid[J].Northern Horticulture,2014,38(02):203.
[7]陈 娟,金文龙,莫亿伟.两种方法提取板栗叶片DNA的效果比较[J].北方园艺,2013,37(18):89.
CHEN Juan,JIN Wen-long,MO Yi-wei.Comparison on Genomic DNA Extracted from the Leaves of Chinese Chestnut by Two Methods[J].Northern Horticulture,2013,37(02):89.
[8]全桂静.苹果采后病害拮抗细菌的筛选与防腐效果研究[J].北方园艺,2013,37(11):130.
QUAN Gui-jing.Study on Screening of Antagonistic Bacteria for Apple Postharvest Diseases and Biocontrol Efficacy[J].Northern Horticulture,2013,37(02):130.
[9]刘国彬,曹均,周连第,等.雄性不育板栗种质杂交结实特性研究[J].北方园艺,2013,37(01):4.
LIU Guo-bin,CAO Jun,ZHOU Lian-di,et al.Study on Pollinated Fruit-setting Characteristics of Male Sterility Germplasm in Chestnut[J].Northern Horticulture,2013,37(02):4.
[10]欧善生,韦荣昌,吴庆华,等.广西凉粉草病虫害种类及防治方法研究[J].北方园艺,2013,37(01):177.
OU Shan-sheng,WEI Rong-chang,WU Qing-hua,et al.Study on Varieties of Diseases and Insect Pests and Control Measures of Mesona chinensis in Guangxi[J].Northern Horticulture,2013,37(02):177.
备注/Memo
第一作者简介：张雪花（1991-），女，湖北宜昌人，硕士研究生，研究方向为板栗采后微生物防治。E-mail：455262713@qq.com. 责任作者：程水源(1965-)，男，湖北天门人，博士，教授，博士生导师，现主要从事经济林种质资源评价与利用等研究工作。E-mail：s_y_cheng@sina.com. 基金项目：湖北省教育厅中青年基金资助项目（Q20132909）；黄冈师范学院产学研合作资助项目（2012025903）；湖北省教育厅高校产学研合作资助重点资助项目(C2010060)；湖北省自然科学基金重点资助项目(2010CBB03901)。