ZHAO Kai,ZHANG Zhihong,LI Jinghui,et al.Research on in vitro Micrografting of Malus hupehensis var.Pingyiensis[J].Northern Horticulture,2016,40(02):88-91.[doi:10.11937/bfyy.201602024]
“平邑甜茶”组织培养微嫁接技术研究
- Title:
- Research on in vitro Micrografting of Malus hupehensis var.Pingyiensis
- Keywords:
- micrograft; apple; Malus hupehensis var.pingyiensis; sRNA transmission
- 文献标志码:
- A
- 摘要:
- 以苹果“平邑甜茶”(Malus hupehensis var.pingyiensis)为微嫁接砧木，“寒富”(Malus domestica cv.‘Hanfu’)为接穗，研究了附加不同浓度6-BA的培养基、接穗不同生理状态、接穗具有叶片数量、不同接穗/砧木组合对嫁接成活率和嫁接口愈合的影响。结果表明：最适于微嫁接的培养基为MS+IAA 0.3 mg/L+BA 1.0 mg/L，选用继代培养40 d的接穗和砧木，并且接穗部分具有2片真叶时的嫁接成活率最高；用“平邑甜茶”作为砧木的嫁接成活率高（81.3%），嫁接口愈合最好。该研究为下一步的转基因sRNA信号在砧木与接穗间的传递运输研究奠定基础。
- Abstract:
- Taking ‘Pingyitiancha’ (Malus hupehensis var.pingyiensis) as rootstock and ‘Hanfu’(Malus domestica cv.‘Hanfu’) as scion，the effect of different concentrations of 6-BA，ages of scion，leaves number of scion，kinds of scion/rootstock on the grafting survival rate was researched.The results showed that MS+IAA 0.3 mg/L+6-BA 1.0 mg/L，rootstocks and scions were cultured for 40 days，with 2 leaves on the scion and ‘Pingyitiancha’ was used as the rootstock，the survival rate of micrografting was 81.3%.Those were the foundation of transferring sRNA from rootstocks to scions.
参考文献/References:
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备注/Memo
第一作者简介：赵恺（1981-），男，博士，讲师，研究方向为果树遗传育种。E-mail：zkpc4@163.com. 基金项目：国家自然科学基金资助项目（31171927）；松原市科技发展计划资助项目（201402）。