[1]刘主,蔡爱群,刘静华,等.基于ITS区序列的疑似野生“南华”草菇菌株的分子鉴定[J].北方园艺,2016,40(01):126-130.[doi:10.11937/bfyy.201601033]
　LIU Zhu,CAI Aiqun,LIU Jinghua,et al.Molecular Identification of Suspected Wild ‘Nanhua’ Volvariella volvacea Strain Based on Internal Transcribed Spacer Sequence[J].Northern Horticulture,2016,40(01):126-130.[doi:10.11937/bfyy.201601033]
点击复制

基于ITS区序列的疑似野生“南华”草菇菌株的分子鉴定

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第40卷 期数: 2016年01 页码: 126-130 栏目: 出版日期: 2016-01-15
Title:
Molecular Identification of Suspected Wild ‘Nanhua’ Volvariella volvacea Strain Based on Internal Transcribed Spacer Sequence
作者:
刘主1; 蔡爱群1; 刘静华1; 何智丹2; 孟晓东1
（1.韶关学院 英东生命科学学院，广东 韶关 512005；华南农业大学 生命科学学院，广东 广州 510640）
Author(s):
LIU Zhu1; CAI Aiqun1; LIU Jinghua1; HE Zhidan2; MENG Xiaodong1
(1.Yindong College of Life Sciences，Shaoguan University，Shaoguan，Guangdong 512005；2.College of Life Sciences，South China Agricultural University，Guangzhou，Guangdong 510640)
关键词:
草菇; 野生菌株; ITS; BLAST; 分子鉴定
Keywords:
Volvariella volvacea; wild strain; ITS sequence; BLAST; molecular identification
DOI:
10.11937/bfyy.201601033
文献标志码:
A
摘要:
以在广东省韶关市“南华寺”周边山岭采得的1株疑似野生“南华”草菇菌株VNH为试材，采用ITS序列克隆和分析，并结合传统形态学研究对其进行分子鉴定。结果表明：菌株VNH为野生“南华”草菇；为进一步研究该野生菌株的生物学特性及驯化栽培研究提供了重要依据；同时，对丰富草菇菌种资源，促进韶关文化和旅游发展具有积极意义。
Abstract:
A suspected wild ‘Nanhua’ Volvariella volvacea was picked beside the mountains next to Nanhua temple in Shaoguan city of Guangdong Province.The pure culture was obtained by tissue isolation and was named strain VNH.The strain was identified by molecular identification with ITS sequence cloning and analyzing and combine with the traditional morphological.The results showed that,VHN strain was wild ‘Nanhua’ V.volvacea.It provided an important basis on the studies of biological characteristics and domestication cultivation for the wild strain.Meanwhile，it had a positive significance to enrich V.volvacea species resources and to promote the development of Shaoguan culture and tourism.

参考文献/References:

 

[1]张树庭,游中骥.草菇及其栽培[M].香港:香港艺美图书公司出版,1978.

[2]龚明,鲍大鹏,汪虹,等.草菇的系统分类地位及分化时间[J].食用菌学报，2013,20(2)：8-11.

[3]韩继刚.草菇生产全书[M].北京:中国农业出版社,2005.

[4]WANG J，GUO L Q，ZHANG K,et al.Highly efficient Agrobacterium-mediated transformation of Volvariella volvacea[J].Bioresource Technology，2008，99：8524-8527.

[5]AHLAWAT〖KG-*3〗 O P，GUPTA P,KAMAL S,et al.Variability in intra-specific and monosporous isolates of Volvariella volvacea based on enzyme activity,ITS and RAPD[J].Indian Journal of Microbiology，2010，50(2)：192-204.

[6]侯军,林晓民,江芸,等.基于ITS序列分析对疑似白羊肚菌株的分子鉴定[J].食品科学，2009，30(5)：141-144.

[7]高磊,徐彪,何刚,等.基于ITS区序列的疑似野生双孢菇菌株的分子鉴定[J].江苏农业科学,2012，40(4)：31-33.

[8]RENSKE L，PAULA L，THOM W K，et al.Molecular identification of ectomycorrhizal mycelium in soil horizons [J].Applied and Environmental Microbiology，2003，69(1)：327-333.

[9]WANG P，LIU Y，YIN Y G，et al.Diversity of microorganisms isolated from the soil sample surround Chroogomphus rutilus in the Beijing region [J].International Journal of Biological Sciences，2011，7(2)：209-220.

相似文献/References:

[1]侯祥保,魏 峰,孔繁成.草菇-双孢菇周年轮作高效栽培技术[J].北方园艺,2014,38(10):143.
　HOU Xiang-bao,WEI Feng,KONG Fan-cheng.Highly Effective Cultivation Technology of Agaricus Bisporus and Straw Mushroom by Annual Rotation[J].Northern Horticulture,2014,38(01):143.
[2]金 卫 根.草菇菌丝体液体培养条件的优化研究[J].北方园艺,2011,35(07):0.[doi:10.11937/bfyy.201107055]
　JIN Wei-gen.Optimization of Straw Mushroom Mycelium Culture Conditions[J].Northern Horticulture,2011,35(01):0.[doi:10.11937/bfyy.201107055]
[3]李 贺 年,王 桂 英,柴 建 明,等.草 菇 高 产 栽 培 技 术[J].北方园艺,2009,33(06):0.[doi:10.11937/bfyy.200906087]
　LI He - nian,W ANG Gui- ying,CHAI Jian - ming,et al.Probe on High Production Cultivation T echnique of Volvariella voluacea[J].Northern Horticulture,2009,33(01):0.[doi:10.11937/bfyy.200906087]
[4]温 海 祥,肖 洪 东,黄 剑 波,等.草菇种内原生质体融合育种研究[J].北方园艺,2000,24(03):0.[doi:10.11937/bfyy.200003037]
　[J].Northern Horticulture,2000,24(01):0.[doi:10.11937/bfyy.200003037]
[5]陆珠,李玉,姚允武.赞比亚引种草菇的发酵料栽培及其效益分析[J].北方园艺,2017,41(06):148.[doi:10.11937/bfyy.201706035]
　LU Zhu,LI Yu,YAO Yunwu.Fermentation Materials Cultivation and Benefit Analysis of Introducing Volvariella volvacea in Zambia[J].Northern Horticulture,2017,41(01):148.[doi:10.11937/bfyy.201706035]
[6]孟 丽,张兰增,周和云,等.数字基因表达谱解析草菇子实体的生长发育[J].北方园艺,2018,42(08):1.[doi:10.11937/bfyy.20173248]
　MENG Li,ZHANG Lanzeng,ZHOU Heyun,et al.Digital Gene Expression Profiling Reveals Volvariella volvacea Fruiting Body Development[J].Northern Horticulture,2018,42(01):1.[doi:10.11937/bfyy.20173248]
[7]王闯,曹娜,刁宁宁,等.日光温室番茄-草菇-小西瓜一年三茬高效栽培模式[J].北方园艺,2019,43(05):200.[doi:10.11937/bfyy.20181641]
　[J].Northern Horticulture,2019,43(01):200.[doi:10.11937/bfyy.20181641]
[8]谷凤举,赖亮民,杜敏如,等.航天诱变处理对草菇生物学特性的影响[J].北方园艺,2022,(17):112.[doi:10.11937/bfyy.20220543]
　GU Fengju,LAI Liangmin,DU Minru,et al.Effects of Space Mutagenesis on Biological Characteristics of Volvariella volvacea[J].Northern Horticulture,2022,(01):112.[doi:10.11937/bfyy.20220543]
[9]查磊,仝宗军,李正鹏,等.隧道发酵技术在草菇工厂化[J].北方园艺,2022,(22):116.[doi:10.11937/bfyy.20220609]
　ZHA Lei,TONG Zongjun,LI Zhengpeng,et al.Application of Tunnel Fermentation Technology in the Preparation of Volvariella volvacea Industrialized Cultivation Materials[J].Northern Horticulture,2022,(01):116.[doi:10.11937/bfyy.20220609]
[10]于海洋,盛春鸽,史磊,等.五个野生黑木耳菌株的驯化栽培及评价研究[J].北方园艺,2024,(14):123.[doi:10.11937/bfyy.20234352]
　YU Haiyang,SHENG Chunge,SHI Lei,et al.Domestication Cultivation and Evaluation of Five Wild Auricularia heimuer Strains[J].Northern Horticulture,2024,(01):123.[doi:10.11937/bfyy.20234352]

备注/Memo

第一作者简介：刘主（1977-），男，湖南宁远人，博士，副教授，现主要从事食用菌和分子生物学的教学与科研等工作。E-mail：liuzhu77@126.com.基金项目：韶关市科技计划资助项目（2013CX/K）；韶关学院大学生创新创业训练计划资助项目（Sycxcy2014-028）。

更新日期/Last Update: 2016-03-02