HU Bingfen,ZHANG Baolin,CAI Guojun,et al.Genetic Diversity by SSR Markers in Seventeen Chinese and Amarican Lycium barbarum L.[J].Northern Horticulture,2016,40(01):90-94.[doi:10.11937/bfyy.201601024]
十七份中美枸杞材料的SSR遗传多样性
- Title:
- Genetic Diversity by SSR Markers in Seventeen Chinese and Amarican Lycium barbarum L.
- Keywords:
- Lycium; SSR markers; genetic diversity
- 文献标志码:
- A
- 摘要:
- 以17份中美枸杞材料为试材，采用SSR分子标记技术对其进行遗传多样性分析，探索中美枸杞的遗传多样性。结果表明：筛选的20对SSR引物扩增得到46个等位变异，平均每对引物扩增得到2.3个等位点，多态性比率达75.44%，多态性信息含量变化范围在0.114 2(SSR111)～0.829 1(SSR22)，平均0.377 2，说明中美枸杞的遗传多样性较低；聚类分析结果显示，在相似系数0.600处，所有供试材料被聚为一类；在相似系数0.730处，美国枸杞和中国枸杞分别独立聚类；在相似系数0.825处，兰山野生枸杞和国内其它枸杞分为2个类群；来自内蒙古和宁夏的枸杞也基本分组，但相似系数很高，说明所有供试材料遗传基础非常狭窄，聚类分析结果与供试材料区域来源有较好的一致性，同一栽培区域育成的品种在不同程度上聚为一类，不同品种间的亲缘关系与地区来源基本相对应。
- Abstract:
- To explore the genetic diversity of Chinese and Amarican Lycium，the genetic diversity of 17 Lycium materials were analyzed using SSR molecular marker technique in this study.The results showed that，46 alleles were amplified by 20 pairs of SSR primers and 2.3 alleles were amplified by per pairs，the ratio of polymorphism was 75.44%，the polymorphism information content ranged from 0.114 2(SSR111) to 0.829 1(SSR22)，with an average of 0.377 2.The diversity of the 17 Lycium materials showed low by these parameters.All the tested materials were clustered into one group based on the UPGMA cluster at the genetice similarity(GS) 0.600；American Lycium and Chinese Lycium were clustered respectively at 0.730；Lanshan wild gouqi and other domestic Lycium were divided respectively into two groups at 0.825；Lycium from Inner Mongolia and Ningxia were grouped roughly，but the GS was very high.It was indicated that the genetic basis of all the tested materials were narrow，and the clustering analysis results of them were consistent with the regional sources of the materials，and the varieties in the same cultivation area were clustered into one category，and the relationship between the cultivars and the region was basically the same.
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备注/Memo
第一作者简介：胡秉芬（1969-），女，甘肃景泰人，博士，副研究员，现主要从事经济林育种等研究工作。E-mail：bf9134hu@163.com.责任作者：张宝琳（1968-），女，硕士，高级工程师，现主要从事经济林等研究工作。E-mail：530139563@qq.com.基金项目：国家林业局“948”资助项目（2011-4-39）。