GUO Dongqi,SHEN Yongjuan.Effect of Water-solube Chitosan on Antagonist Metschnikowia pulcherrima[J].Northern Horticulture,2015,39(24):127-130.[doi:10.11937/bfyy.201524035]
水溶性壳聚糖对美极梅奇酵母菌拮抗效力的影响
- Title:
- Effect of Water-solube Chitosan on Antagonist Metschnikowia pulcherrima
- 文献标志码:
- A
- 摘要:
- 以冬枣为试材，采用体外及体内试验方法，研究了水溶性壳聚糖对美极梅奇酵母菌抑制冬枣采后贮藏保鲜中青霉病的效果的影响。结果表明:水溶性壳聚糖对橘青霉孢子的萌发有显著的抑制作用，壳聚糖浓度>0.3%（w/v），橘青霉孢子萌发状态完全受到抑制；在NYDB培养基（液体）中，壳聚糖的浓度≥0.04%（w/v）时，对酵母菌的生长有较强的抑制效果，在NYDA固体培养基中，壳聚糖浓度在0.1％~0.9%（w/v）范围内对其均有显著抑制作用；在冬枣伤口处，壳聚糖浓度≤0.1%时，对酵母菌的生长无显著影响；美极梅奇酵母菌与浓度为0.1%（w/v）壳聚糖结合处理对冬枣青霉病的抑制效果最佳。
- Abstract:
- Taking winter jujubes as test material，the inhibiting effect of Metschnikowia pulcherrima on control，blue mould of winter jujube by using water-soluble chitosan was studied by adopting the method of in vitro and in vivo tests.The results showed that the water-soluble chitosan could significantly inhibit germination of Penicillium citrinum spore，when the concentration of water-soluble chitosan was more than 0.3% (w/v)，the germination of P.citrinum spore was inhibited completely.In NYDB medium，the concentration of water-soluble chitosan was 0.04% (w/v) or more，the growth of M.pulcherrima was strongly inhibited，while in NYDA (solid medium)，the growth of M.pulcherrima was significantly inhibited in the range of 0.1%-0.9% (w/v) water-soluble chitosan concentration.In the wound of winter jujube，the water-soluble chitosan concentration of 0.1% or less had no significant effect on the growth of M.pulcherrima，the combination of water-soluble chitosan concentration of 0.1% with M.pulcherrima had the best inhibiting effect on blue mould of winter jujube.
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备注/Memo
第一作者简介：郭东起（1975-），男，河南南阳人，硕士，副教授，现主要从事食品安全及果蔬保鲜与加工技术等研究工作。E-mail:guodongqi10@126.com.基金项目：国家自然科学基金资助项目（31160342）。