LIU Yujie,GUO Danni,ZHANG Yu,et al.Cloning and Sequence Analysis of RNase-like Gene From Citrus grandis var.Shatinyu Hort[J].Northern Horticulture,2015,39(24):70-74.[doi:10.11937/bfyy.201524021]
沙田柚RNase-like基因的克隆及序列分析
- Title:
- Cloning and Sequence Analysis of RNase-like Gene From Citrus grandis var.Shatinyu Hort
- 关键词:
- 沙田柚; RNase-like基因; 序列分析
- 文献标志码:
- A
- 摘要:
- 以沙田柚自交和异交花柱为试材，采用高通量测序技术对其进行转录组测序。通过差异分析得到RNase-like贮藏蛋白的基因序列，并研究了其理化性质。结果表明：该基因全长1 048 bp（GenBank登录号为KR363153)，开放阅读框(ORF)全长为693 bp，共编码230个氨基酸，编码的蛋白质的分子量为25.82 kDa，理论等电点为5.30，含有核苷酸结合的保守区域，属于 RNase-T2 超家族成员。该蛋白为疏水性蛋白，可能的磷酸化位点共有15个。氨基酸序列同源性分析表明，该基因编码的氨基酸与甜橙和克莱门柚的同源性均为99%。系统进化树显示沙田柚RNase-like贮藏蛋白基因与甜橙，克莱门柚的亲缘关系较近，属于同一进化分支。
- Abstract:
- The self-pollinated and cross-pollinated style of Citrus grandis var.Shatinyu Hort were used as test materials and transcriptome sequenced by high-throughput sequencing technology.The sequence of RNase-like storage protein was obtained by differential analysis，what’s more，the physical and chemical properties of this protein were studied.The result showed that,the length of this gene(GenBank accession number：KP172529) was 1 048 bp with an open reading frame(ORF)of 693 bp，encoding 290 amino acids with deduced molecular weight of 25.82 kDa，and the theoretical pI value was 5.30.And it belonged to the RNase-T2 superfamily with a conserved region binding the nucleotide.This protein was a hydrophobic protein，and there were 15 phosphorylation sites within the polypeptide chain.The homology analysis of amino acid sequence indicated that the RNase-like storage protein shared high homology with Citrus sinensis(99%) and Citrus clementina(99%).The phylogenetic tree revealed that RNase-like gene storage protein showed closer kinship with Citrus sinensis and Citrus clementina，indicating that they belonged to the same evolutionary branch.
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备注/Memo
第一作者简介：刘玉洁（1990-），女，硕士研究生，研究方向为植物生殖生理。E-mail：2510965486@qq.com.责任作者：秦新民（1956-），男，博士，教授，现主要从事植物分子生物学等研究工作。E-mail：xmain@mailbox.gxnu.edu.cn.基金项目：国家自然科学基金资助项目（31360477）；广西教育厅资助项目（2013YB036）。