[1]吴清山.猴头菇菌丝体诱变提高多糖产量的培养基优化试验[J].北方园艺,2015,39(21):146-149.[doi:10.11937/bfyy.201521037]
　WU Qingshan.Medium Optimization Test of Improving Polysaccharide Yield by Hericium mycelium Mutation[J].Northern Horticulture,2015,39(21):146-149.[doi:10.11937/bfyy.201521037]
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猴头菇菌丝体诱变提高多糖产量的培养基优化试验

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第39卷 期数: 2015年21 页码: 146-149 栏目: 出版日期: 2015-11-15
Title:
Medium Optimization Test of Improving Polysaccharide Yield by Hericium mycelium Mutation
作者:
吴清山
（滨州职业学院，山东滨州 256603）
Author(s):
WU Qingshan
(Binzhou Polytechnic，Binzhou，Shandong 256603)
关键词:
猴头菇菌丝体诱变; 菌丝体干重; 胞内外多糖; 正交实验
Keywords:
Hericium erinaceum:mycelium mutation; intercellulre and extracellular polysaccharide; orthogonal test
DOI:
10.11937/bfyy.201521037
文献标志码:
A
摘要:
以猴头菇菌丝体为试验材料，紫外线诱变后，以菌丝体生物量和多糖含量为指标，筛选优质菌株，运用液体深层发酵的方法培养筛选出的优质菌株菌丝体，通过单因素试验和正交实验研究了规模化生产常用碳源、氮源对菌丝体生物量和多糖产量的影响。结果表明：911-3为最佳菌株；表现优异碳源为蔗糖和高粱粉，表现优异氮源为麸皮和米糠；深层发酵的最佳碳源、氮源组合为蔗糖0.4%、高粱粉0.4%、麸皮0.3%、米糠0.3%。
Abstract:
Taking Hericium erinaceus mycelium as experimental material，after ultraviolet radiation，mycelial biomass and polysaccharides content were selected as the indexes，screening good strains using submerged fermentation method for culturing and screening out the mycelium，through single factor and orthogonal test,the influence of scale commonly used in the production of carbon source and nitrogen source on the production of mycelial biomass and polysaccharides were studied.The results showed that the 911-3 was the best strain；excellent carbon source was sucrose and sorghum flour，the excellent performance of nitrogen source was wheat bran and rice bran；submerged fermentation was the best carbon source，nitrogen source combination for 0.4% of sucrose，sorghum flour 0.4%，wheat bran 0.3% of rice bran 0.3%.

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备注/Memo

作者简介：吴清山(1976-)，男，本科，助理研究员，研究方向为食药用菌驯化和栽培及技术推广等工作。E-mail：wqs9619@126.com.

更新日期/Last Update: 2015-12-28