WEI Hailian,LIU Zhixi,ZHANG Wanjie,et al.Analysis of Genetic Diversity of Wild Lepista nuda Population From Zhaotong，Yunnan[J].Northern Horticulture,2015,39(17):132-136.[doi:10.11937/bfyy.201517035]
云南昭通野生紫丁香蘑群体的遗传多样性分析
- Title:
- Analysis of Genetic Diversity of Wild Lepista nuda Population From Zhaotong，Yunnan
- Keywords:
- Lepista nuda; internal transcribed spacers (ITS); single nucleotide polymorphism (SNPs); genetic diversity
- 文献标志码:
- A
- 摘要:
- 以采自云南昭通的66株野生紫丁香蘑（Lepista nuda，L.nuda)为研究对象，分析了其ITS序列及其单核苷酸多态性(SNP)。结果表明：经结合形态和分子水平的鉴定，确定所采的野生蘑菇为紫丁香蘑；66个紫丁香蘑的 ITS序列长度变化范围为666～671 bp，ITS1为241～244 bp，5.8S为155 bp，ITS2为267～275 bp，G+C含量的变化范围为41.5%～42.4%，所有样本的平均遗传距离为0.003 0；通过序列比对，共筛选出7个SNP位点，单突变位点3个，简约信息位点4个；转换与颠换的比值R为0.672；该群体的核酸多态性指数Pi为0.002 91，每位点核苷酸多态性指数 Theta(per site from Eta)为0.002 36。采用邻接法(neighbour joining，NJ)构建的系统发育树分支支持率低，表明群体内各菌株遗传距离小，该群体的遗传多样性丰度较低。综上说明云南昭通野生紫丁香蘑群体的遗传多样性水平很低，需要加强保护。
- Abstract:
- Lepista nuda from Zhaotong,Yunnan Province was used as the research object，its ITS sequence and single nucleotide polymorphism (SNP) were analyzed.The results showed that：combined with morphological and molecular identification，the collected wild mushrooms were confirmed as Lepista nuda；66 strains L.nuda’s ITS sequence length was 666-671 bp，ITS1:241-244 bp，5.8S:155 bp，ITS2:267-275 bp；G+C content was 41.5%-42.4%；the average genetic distance of all samples was 0.003 0.By sequence alignment，seven SNPs loci were screened out：three singleton variable sites，four parsimony informative sites；transition and transversion ratio was 0.672.The nucleic acid polymorphisms index Pi of the population was 0.002 91.Each site nucleotide polymorphisms index Eta was 0.002 36.Neighbor (neighbour-joining，NJ) phylogenetic tree was constructed.Bootstrap support rate was low，which indicated that genetic distance of each strain in the population was very close and their genetic diversity was very low.In summary，the genetic diversity of the wild Lepista nuda population from Zhaotong was very low so that its protection should be strengthened.
参考文献/References:
[1]陈士俞.食用菌生产大全[M].北京:中国农业出版社,1988. [2]谢支锡,王云,王柏,等.长白山伞菌图志[M].长春:吉林科学技术出版社,1986. [3]陈士瑜,陈惠.菇菌栽培手册[M].北京:科学技术文献出版社,2003. [4]YANG S,HAN J Y,JOO E Y,et al.Study on the antitumor effects of extmcts from Lepista nuda mushroom[J].Journal of the Korean Society of Food Science and Nutritio,2005,3(34)：317-322. [5]KIM S D,KIM J H,KIM J B,et al.Mycelial culture conditions of Lepista nuda and extracellular enzyme activity[J].The Korean Journal of Microbiology,2005,3(6):42. [6]史全良,赵卫国.桑树ITS序列测定及特点的初步分析[J].蚕业科学，2001,27(2):140-141. [7]FRUDAKIS T,VENKATE S K.A classier for the SNP-based inference of ancestry[J].Journal of Forensic Sciences,2003,48(4)：771-782. [8]GAO Z X,YAN L,WANG X F,et al.Sodium nitroprusside (SNP) alleviates the oxidative stress induced by NaHCO3 and protects chloroplast from damage in cucumber[J].African Journal of Biotechnology,2012,11(27):6974-6982. [9]吴发红,黄东益,黄小龙,等.几种真菌DNA提取方法的比较[J].中国农学通报,2009,25(8):62-64. [10]HALL T A.BioEdit:a user friendly biological sequence alignment editor and analysis program for windows 95/98/NT[J].Nucleic Acids Symposium Series,1999,41:95-98. [11]WANG L,ROOSINCK M J.Comparative analysis of expressed sequences reveals a conserved pattern of optimal codon usage in plants[J].Plant Molecular Biology,2006,61(4-5):699-710. [12]LARKIN M A,BLACKSHIELDS G,BRONW N P,et al.Clustal W and clustal X version 2.0[J].Bioinformatics,2007,23(21):2947-2948. [13]KUMAR S,NEI M,DUDLEY J,et al.MEGA:a biologist-centric software for evolutionary analysis of DNA and protein sequences[J].Briefbioinform,2008,9(4):299-306. [14]LIBRADA P,ROZAS J.DnaSP v5：A Software for comprehensive analysis of DNA polymorphism data[J].Bioinformatics,2009,25(11):1451-1452. [15]PLOTREE D,PIOTGRAM D.Phylip-phylogeny inference package (version 3.2)[J].J Felsenstein Cladistics,1989,5(1):164-166. [16]LANDEWEERT R,LEEFLANG P,KUYPER T W,et al.Molecular identification of ectomycorrhizalm mycelium in soil horizons[J].Appliedand Environmental Microbiology,2003,69(1):327-333. [17]谢富刚,刘雁英,邱慧兰.紫丁香蘑菌丝体生物学特性研究初报[J].现代农业科技,2010(2):72-76. [18]高锦明,董泽军.紫丁香蘑的化学成分[J].中草药,2002,33(5):398-401. [19]周峰,王瑞娟,李玉,等.珍稀食药用菌紫丁香蘑的研究进展[J].食用菌学报,2010,17(4):79-83. [20]赵婷.紫丁香蘑菌丝体培养特性及人工驯化栽培研究[D].延边:延边大学,2010. [21]李萌伟,李芳菲,樊志宏,等.紫丁香蘑 ISSR-PCR反应体系的正交优化与建立[J].生物技术,2013,23(6):55-59. [22]LEE Y S,KIM N W,KIM J B.Genetic variation based on random amplified polymorphic DNA (RAPD) and internal transcribed spacer (ITS) region sequences in Lepista nuda[J].Journal of the Korean Society of Food Science and Nutrition,2012,22(11):1470-1476.
相似文献/References:
[1]董平,赵培宝,任爱芝.蝴蝶兰灰霉病菌的分离鉴定[J].北方园艺,2014,38(04):109.
DONG Ping,ZHAO Pei-bao,REN Ai-zhi.Isolation and Identification of Botrytis cinerea?in Phalaenopsis[J].Northern Horticulture,2014,38(17):109.
[2]欧立军,孙海军.基于ITS序列的蔷薇纲二十六个物种遗传多样性分析[J].北方园艺,2013,37(11):94.
OU Li-jun,SUN Hai-jun.Genetic Diversity Analysis of Twenty-six Species in Rose Class Based on ITS Sequences[J].Northern Horticulture,2013,37(17):94.
[3]刘 洋,孙来玉,陆云华,等.九株蛹虫草菌株的生物学性状研究[J].北方园艺,2013,37(20):140.
LIU Yang,SUN Lai-yu,LU Yun-hua,et al.Study on the Biological Characteristics of Nine Cordyceps militaris Strains[J].Northern Horticulture,2013,37(17):140.
[4]严雪瑞,周源,赵睿杰,等.蓝莓叶穿孔病菌鉴定及其生物学特性研究[J].北方园艺,2014,38(16):123.
YAN Xue-rui,ZHOU Yuan,ZHAO Rui-jie,et al.Study on Identification and Biological Characteristics of Caused Agent of Leaf Perforation on Blueberry[J].Northern Horticulture,2014,38(17):123.
[5]李 蝶,吴 浩,李红玉,等.野生泡囊侧耳的鉴定及其驯化栽培[J].北方园艺,2015,39(04):143.[doi:10.11937/bfyy.201504034]
LI Die,WU Hao,LI Hong-yu,et al.Domestication and Cultivation of Wild Pleurotus Cystidiosus and Its Molecular Identification[J].Northern Horticulture,2015,39(17):143.[doi:10.11937/bfyy.201504034]
[6]刘振东,薛蓓,王泽敏,等.林芝地区两株优良黑木耳菌株的ITS序列验证[J].北方园艺,2015,39(10):98.[doi:10.11937/bfyy.201510024]
LIU Zhen-dong,XUE Bei,WANG Ze-min,et al.Verification of Two Excellent Auricularia auricula Species Based on Internal Transcribed Spacer(ITS) Sequences in Nyingchi Area[J].Northern Horticulture,2015,39(17):98.[doi:10.11937/bfyy.201510024]
[7]刘主,蔡爱群,刘静华,等.基于ITS区序列的疑似野生“南华”草菇菌株的分子鉴定[J].北方园艺,2016,40(01):126.[doi:10.11937/bfyy.201601033]
LIU Zhu,CAI Aiqun,LIU Jinghua,et al.Molecular Identification of Suspected Wild ‘Nanhua’ Volvariella volvacea Strain Based on Internal Transcribed Spacer Sequence[J].Northern Horticulture,2016,40(17):126.[doi:10.11937/bfyy.201601033]
[8]朱畇昊,杜真辉,董诚明,等.地黄块根内生真菌的多样性研究[J].北方园艺,2016,40(03):145.[doi:10.11937/bfyy.201603041]
ZHU Yunhao,DU Zhenhui,DONG Chengming,et al.Diversity of Endophytic Fungi From Tubers of Rehmannia glutinosa Libosch[J].Northern Horticulture,2016,40(17):145.[doi:10.11937/bfyy.201603041]
[9]钱雪婷,陈文强,邓百万,等.基于ITS序列分析秦巴山区黑木耳菌株的遗传多样性[J].北方园艺,2016,40(12):147.[doi:10.11937/bfyy.201612037]
QIAN Xueting,CHEN Wenqiang,DENG Baiwan,et al.Analysis of the Genetic Diversity of Auricularia auricular Strains From Qinba Mountains Based on ITS Sequence[J].Northern Horticulture,2016,40(17):147.[doi:10.11937/bfyy.201612037]
[10]范莉莉,刘伟,郭守玉,等.六株扁枝衣内生真菌的抗病原真菌活性[J].北方园艺,2017,41(06):117.[doi:10.11937/bfyy.201706027]
FAN Lili,LIU Wei,GUO Shouyu,et al.Antifungal Activities of Six Endophytic Fungi From Evernia mesomorpha[J].Northern Horticulture,2017,41(17):117.[doi:10.11937/bfyy.201706027]
备注/Memo
第一作者简介：魏海莲(1989-)，女，硕士研究生，研究方向为微生物学。E-mail：771453668@qq.com.责任作者：李翠新（1972-），女，博士，副教授，研究方向为微生物学与森林保护学。E-mail：cuixinheli@163.com.基金项目：云南省优势特色重点学科生物学一级学科建设资助项目（50097505）；云南省应用基础研究计划资助项目(2011FZ141)；西南地区生物多样性保育国家林业局重点实验室开放基金资助项目（KL200707）；云南省高校特色专业建设点资助项目（50116001)。