CHEN Zongli,ZHANG Xiangqian,LIU Shipeng,et al.Optimization of the Jujube Polysaccharide Extraction Technology and Analysis of Polysaccharide Content in Twenty-five Jujube Varieties From Northern Shaanxi[J].Northern Horticulture,2015,39(17):110-114.[doi:10.11937/bfyy.201517029]
枣多糖提取工艺优化及陕北二十五个 品种枣多糖含量分析
- Title:
- Optimization of the Jujube Polysaccharide Extraction Technology and Analysis of Polysaccharide Content in Twenty-five Jujube Varieties From Northern Shaanxi
- 关键词:
- 陕北枣; 多糖含量; 超声波辅助提取工艺; 紫外-可见分光光度法
- Keywords:
- Jujube from Northern Shaanxi; polysaccharide content; ultrasonic assisted extraction technology; ultraviolet-visible spectrophotometry
- 文献标志码:
- A
- 摘要:
- 以延川县产大木枣为试材，采用正交实验设计研究了超声波辅助提取枣多糖的优化工艺；用筛选出的优化工艺，结合紫外-可见分光光度法分析了25个品种、27个样品枣的多糖含量。结果表明：超声波辅助提取枣多糖的优化工艺为超声波功率28 Hz，料液比1∶40 g/mL，提取温度60℃，提取时间40 min，pH 7.0。27个样品枣粉的平均纯多糖含量为220.18±52.87 mg/g；经F检验，枣品种间多糖含量存在极显著差异，最高者与最低者相差达2.38倍。大木枣、狗头枣和晋枣是多糖含量较高的地方优良品种，雪枣是多糖含量较高的引进多年的适应性良种，这些枣品种是当地种植和品质育种的优选亲本。该研究结果为陕北红枣多糖的开发利用及品种改良与种植提供了科学的基础研究数据。
- Abstract:
- With Yanchuan-produced jujube (Damuzao) as raw materials，optimize technology conditions of extraction jujube polysaccharide were studied with using the ultrasound assisted methods by orthogonal experimental design；polysaccharide content of 25 varieties，27 jujube samples were analyzed to screen out the optimization technology combinating with ultraviolet-visible spectro-photometry.The results showed that the optimum process conditions of extracted jujube polysaccharide by ultrasonic assisted were ultrasonic power of 28 Hertz，solid-liquid ratio of 1∶40 g/mL，extracting temperature of 60℃，extracting time of 40 min and pH 7.0.The anverage pure polysaccharide content was 220.18±52.87 mg/g in 27 sample date powder.F test indicated that polysaccharide content among these jujube cultivars there were extremely significant differences，the highest and the lowest polysaccharide content discrepancy reach 2.38 times.The jujube mill ‘Damuzao’,‘Goutouzao’and‘Jinzao’were local fine varieties with high polysaccharide content and the jujube mill‘Xuezao’was a daptive improved varieties of higher polysaccharide content introduced for many yeas；these jujube varieties are optimizing parent of locally grown and quality breeding.These researched results provided scientific basis for the development and utilization of Northern Shaanxi jujube polysaccharide and variety improving and planting.
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备注/Memo
第一作者简介：陈宗礼(1954-)，男，陕西扶风人，本科，教授，硕士生导师，现主要从事植物遗传育种与生物技术等研究工作。E-mail：yadxczl@126.com.基金项目：陕西省科技统筹创新工程计划资助项目（2013KTZB02-03-01）；陕西省教育厅重点实验室科学研究计划资助项目(13JS125，14JS114)。