ZHANG Wei,TIAN Yike,KONG Xianghua,et al.Expression Analysis of Apple Chitinase Gene Induced by Marssonina mali in Germplasms With Different Resistances[J].Northern Horticulture,2015,39(17):104-107.[doi:10.11937/bfyy.201517027]
不同抗性种质上褐斑病菌诱导的苹果几丁质酶基因表达特征分析
- Title:
- Expression Analysis of Apple Chitinase Gene Induced by Marssonina mali in Germplasms With Different Resistances
- 关键词:
- 苹果几丁质酶基因（MdChi）; 褐斑病菌; 表达分析
- 文献标志码:
- A
- 摘要:
- 植物几丁质酶通过降解真菌细胞壁起到抵抗真菌病害的作用，是一种重要的病程相关蛋白。现以苹果品种‘富士’、‘秦冠’及其杂交后代为试材，采用荧光定量PCR的方法，研究接种苹果褐斑病对苹果几丁质酶基因MdChi表达的影响。结果表明：褐斑病菌诱导下MdChi在3份种质的叶片中均有表达，感病品种‘富士’叶片中MdChi基因表达量明显高于抗病品种‘秦冠’及二者杂交后代‘FQ-2’。侵染后24 h时‘富士’叶片中的MdChi表达量明显上升，48 h后表达量又迅速下降。而抗病品种‘秦冠’及抗病杂种‘FQ-2’中MdChi基因表达量在侵染后的96 h内未有较大幅度变化，均保持在较低水平。
- Abstract:
- Plant chitinase，one of the important pathogenesis-related (PR) proteins in plants，has the effect of resistance to fungal diseases by degradating the cell walls of fungi.In this study，leaves of apple cultivar ‘Fuji’，‘Qinguan’ and one selection derived from their hybirdization ‘QF-2’ were used as materials.With quantitative real-time PCR method,expression of apple chitinase gene MdChi in response to marssonina blotch was analysed.The results showed that MdChi gene was expressed in all these three germplasms.The expression quantity in the susceptible cultivar ‘Fuji’ was significantly higher than the resistant cultivar ‘Qinguan’ and their hybrid ‘FQ-2’.Twenty-four hours after the inoculation，the expression level in ‘Fuji’ leaves increased obviously，and then sharply declined.However，the expression of this gene maintained at low levels and there was no significant change in both of the resistant cultivar ‘Qinguan’ and the resistant hybird ‘FQ-2’ during 96 hours after the inoculation.
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备注/Memo
第一作者简介：张伟（1988-），男，硕士研究生，研究方向为果树生物技术与遗传育种。E-mail:z20081288@126.com.责任作者：田义轲（1967-），男，博士研究生，教授，硕士生导师，现主要从事果树遗传育种等研究工作。E-mail:yktian@qau.edu.cn.基金项目：山东省科技发展计划资助项目（2014GNC110017）。